Rhizosphere Microbiome Regulates the Growth of Mustard under Organic Greenhouse Cultivation
Round 1
Reviewer 1 Report
Please find the comments and suggestions for the manuscript entitled "Rhizosphere microbiome regulates the growth of mustard under organic greenhouse cultivation"
- Authors were reporting that the experiment was conducted in the greenhouse conditions but there was no mention of conditions such as temperature humidity light etc---
- Secondly, it is not clear about what was the pot size or container size on which the seedlings were transferred –
- Please also mention how many replications were there, please provide all the details regarding the experimental design—
- Section 2.3 it is not clear from the description that pH was measured from the soil used in the experiment if so please mention
- There was no mention of any citation for available P was determined by molybdenum blue colorimetric method -- please include the same.
- As per the methods, the authors performed sampling only on 7 days but there is no second-time point. Is there any specific reason for the same—
- Please present the figures in better representative form- Figures 1 to 3 more clear and figure 4 with distinct axis's –
- Section 3.1 here some part belongs to methods section which is already described, therefore it is suggested to remove or move the same to methodology and include only results portion in the results section.
- Please include a separate section for the conclusion and include lines 329 to 337 under that subheading will be more justified
Author Response
Please see the attachment.
Author Response File: 
Author Response.docx
Reviewer 2 Report
Dear Authors,
The subject of the study is interesting and topical, with high scientific and practical importance.
The introduction is presented correctly, in accordance with the subject. Numerous scientific articles, in concordance to the topic of the study, were consulted.
Methodology of the study was clearly presented, and appropriate to the proposed objectives.
The obtained results are important and have been analyzed and interpreted correctly, in accordance with the current methodology.
The discussions are appropriate, in the context of the results, and was conducted compared to other studies in the field.
The scientific literature, to which the reporting was made, is recent and representative in the field.
Some suggestions and corrections were made in the article.
The following aspects are brought to the attention of the authors.
1.
Supplementary material
Figure S2, Figure title
“rarefied” instead of “rarefield”
It is not recommended "." (point) at the end of the title of the figures
2.
Italic Font style for species names
Eg
Page 3, row 118
"(Brassica juncea cv. yuanjen)" instead of "(Brassica juncea cv. yuanjen)"
Page 8, row 264
"Phaseolus vulgaris" instead of "Phaseolus vulgaris"
More suggestions and corrections were made in the article
3.
Conclusions
The Conclusions chapter is missing.
According to Instructions for Authors, and Microsoft Word template, Agriculture journal,
“This section is not mandatory but can be added to the manuscript if the discussion is unusually long or complex.”
Regarding the Conclusions chapter, it is recommended that the authors analyze this aspect, and proceed accordingly, for the most appropriate version of the article.
4.
References
The entire chapter References needs to be revised, according to Instructions for Authors, and Microsoft Word template, Agriculture journal.
“
- Author 1, A.B.; Author 2, C.D. Title of the article. Abbreviated Journal Name Year, Volume, page range.”
“Include the digital object identifier (DOI) for all references where available.”
Samples
“van der Heijden, M.G.A.; Bardgett, R.D.; van Straalen, N.M. The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems. Ecol. Lett. 2008, 11, 296-310. Doi: 10.1111/j.1461-0248.2007.01139.x”
Instead of
”van der Heijden, M. G. A., Bardgett, R. D., and van Straalen, N. M. 2008. The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems. Ecol. Lett. 11, 296–310.”
“Walters, K.E.; Martiny, J.B.H. Alpha-, beta-, and gamma-diversity of bacteria varies across habitats. PLoS One 2020, 15(9), e0233872. https://doi.org/10.1371/journal.pone.0233872”
Instead of
“Walters, K. E., and Martiny, J. B. 2020. Alpha-, beta-, and gamma-diversity of bacteria varies across habitats. PLoS One 15(9):e0233872”
It is recommended to pay attention to,
Correct and complete names of the authors
Abbreviated Journal Name – font style Italic
Year – font style Bold
Volume number – font style Italic
Doi number
It is recommended that each bibliographic source be reviewed and corrected in accordance with the Instructions for Authors, and Microsoft Word template, Agriculture journal
Comments for author File: 
Comments.pdf
Author Response
Please see the attachment.
Author Response File: Author Response.docx
Reviewer 3 Report
The manuscript contains interesting research results. Congratulations to the Authors! However, it requires minor corrections.
Comments:
Citation: Lastname, F.; Lastname, F.; Lastname, F. Title ?
Line 86: Please provide the characteristics of the variety.
Line 86: Please provide the place of research. In which year was the experiment conducted?
Line 90, 91: correct P not P2O5, K not K2O
Line 92: correct kg/m2,
Author Response
Please see the attachment.
Author Response File: Author Response.docx
Round 2
Reviewer 1 Report
The comments and suggestions on the manuscript entitled" Rhizosphere microbiome regulates the growth of mustard under organic greenhouse cultivation have been well addressed by the authors. The current version is better and well worked and presented by the authors. The changes done in the figures and texts are satisfactory. The inclusions in the materials and methods are fine. I have no further suggestions and recommendations.
Thanks for the revised version.
