N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Groups and Ethics
2.2. Patient Characteristics and Collection of Serum Samples
2.3. Collection of Unstimulated Whole Saliva (UWS)
2.4. Detection of Blood Sample Immunoglobulin A (IgA) Level
2.5. Detection of Salivary IgA Level
2.6. Statistical Analysis
3. Results
3.1. Determination of Serum and Salivary IgA Levels
3.2. Unstimulated Whole Saliva (UWS) and APSCT
3.3. Correlation of Serum and Salivary IgA Levels and Salivary Flow Rate with Oral Mucositis
3.4. Comparison of Serum IgA N-glycome Profile of Controls and Patients Undergoing APSCT
3.5. Comparison of Salivary IgA N-glycome Profiles in Healthy Controls and Patients Undergoing APSCT
3.6. Sialoform to Neutral Carbohydrate Ratio (SF/NF) in Serum and Saliva
4. Discussion
4.1. Salivary Flow Rate
4.2. Decreased Serum IgA during APSCT
4.3. Decreased Salivary IgA Secretion Rate
4.4. Analysis of Serum IgA N-glycome Profile
4.5. Analysis of the Salivary IgA N-glycome Profile
4.6. Examination of the Sialoform to Neutral Carbohydrate Ratio (SF/NF) in Serum and Saliva
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ANOVA | one-way analysis of variance |
APSCT | autologous peripheral stem-cell transplantation |
BEAM | BCNU, etoposide, cytosine arabinoside, melphalan |
BMT | bone marrow transplantation |
CMR | complete morphologic remission |
E2 | estradiol |
EDTA | ethylenediaminetetraacetic acid |
ELISA | enzyme linked immunosorbent assays |
FA2BG2S2 | core fucosylated, sialylated bisecting biantennary glycan |
GCF | gingival crevicular fluid |
GVHD | graft-versus-host disease |
HL | Hodkin lymphoma |
Ig | immunoglobulin |
IgA | immunoglobulin A |
IgG | immunoglobulin G |
sIgA | secretory immunoglobulin A |
LPS | lipopolysaccharide |
MM | multiple myeloma |
NHL | non-Hodgkin lymphoma |
OM | oral mucositis |
PCA | principal component analysis |
PCs | plasma cells |
PR | partial remission |
SC | secretory component |
SF/NF | sialoform to neutral carbohydrate ratio |
Z(IgA1) | IgA binding affibody protein derived from the Z-domain of Staphylococcal protein A |
UWS | unstimulated whole saliva |
VGPR | very good partial remission |
WHO | World Health Organization |
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Gebri, E.; Kovács, Z.; Mészáros, B.; Tóth, F.; Simon, Á.; Jankovics, H.; Vonderviszt, F.; Kiss, A.; Guttman, A.; Hortobágyi, T. N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis. J. Clin. Med. 2020, 9, 1747. https://doi.org/10.3390/jcm9061747
Gebri E, Kovács Z, Mészáros B, Tóth F, Simon Á, Jankovics H, Vonderviszt F, Kiss A, Guttman A, Hortobágyi T. N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis. Journal of Clinical Medicine. 2020; 9(6):1747. https://doi.org/10.3390/jcm9061747
Chicago/Turabian StyleGebri, Enikő, Zsuzsanna Kovács, Brigitta Mészáros, Ferenc Tóth, Ádám Simon, Hajnalka Jankovics, Ferenc Vonderviszt, Attila Kiss, András Guttman, and Tibor Hortobágyi. 2020. "N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis" Journal of Clinical Medicine 9, no. 6: 1747. https://doi.org/10.3390/jcm9061747
APA StyleGebri, E., Kovács, Z., Mészáros, B., Tóth, F., Simon, Á., Jankovics, H., Vonderviszt, F., Kiss, A., Guttman, A., & Hortobágyi, T. (2020). N-Glycosylation Alteration of Serum and Salivary Immunoglobulin A Is a Possible Biomarker in Oral Mucositis. Journal of Clinical Medicine, 9(6), 1747. https://doi.org/10.3390/jcm9061747