Next Article in Journal
Knowledge, Attitudes, Practices, and Burden During the COVID-19 Pandemic in People with Parkinson’s Disease in Germany
Previous Article in Journal
Estimating the Risk of COVID-19 Death during the Course of the Outbreak in Korea, February–May 2020
Open AccessArticle

Downregulated Platelet miR-1233-5p in Patients with Alzheimer’s Pathologic Change with Mild Cognitive Impairment is Associated with Aβ-Induced Platelet Activation via P-Selectin

1
College of Pharmacy, Ajou University, Suwon 16499, Korea
2
Research Institute of Pharmaceutical Sciences and Technology, Ajou University, Suwon 16499, Korea
3
Department of Biomedical Sciences, School of Medicine, Ajou University, Suwon 16499, Korea
4
Department of Psychiatry, School of Medicine, Ajou University, Suwon 16499, Korea
*
Authors to whom correspondence should be addressed.
J. Clin. Med. 2020, 9(6), 1642; https://doi.org/10.3390/jcm9061642
Received: 20 April 2020 / Revised: 27 May 2020 / Accepted: 28 May 2020 / Published: 29 May 2020
(This article belongs to the Section Molecular Diagnostics)
MicroRNAs (miRNAs) have been proposed as a promising biomarker for various diseases including Alzheimer’s disease (AD). More attention has recently been focused on the diagnosis and treatment at earlier stage of mild cognitive impairment (MCI) for preventing its progression to AD. To identify potential pathologic markers for Aβ(+)MCI (Alzheimer’s pathologic change with MCI), we investigated miRNA expression profiles in the platelets from patients with Aβ(+)MCI, in comparison with those from Aβ(−)MCI (Non-Alzheimer’s pathologic change with MCI) and CNI (cognitively normal individuals). We found that let-7i-5p, miR-125a, miR-1233-5p, and miR-6787-5p were significantly downregulated, while miR-6880-5p expression was upregulated. Of these, only miR-1233-5p was significantly downregulated by Aβ treatment in both human platelets and their precursor megakaryocytes (MEG-01 cells). We explored the role of miRNAs by using miRNA mimics or inhibitors, and found that the diminished level of miR-1233-5p was associated with Aβ-induced increase in the expression of P-selectin and cell adhesion to fibronectin. Our results further indicated that Aβ-induced increase in platelet/MEG adhesion to fibronectin is likely mediated via P-selectin. In conclusion, this study suggests the downregulation of platelet-derived miR-1233-5p as a pathologic marker for Aβ(+)MCI. View Full-Text
Keywords: mild cognitive impairment; platelets; miRNA; amyloid-beta; P-selectin mild cognitive impairment; platelets; miRNA; amyloid-beta; P-selectin
Show Figures

Figure 1

MDPI and ACS Style

Lee, B.K.; Kim, M.H.; Lee, S.Y.; Son, S.J.; Hong, C.H.; Jung, Y.-S. Downregulated Platelet miR-1233-5p in Patients with Alzheimer’s Pathologic Change with Mild Cognitive Impairment is Associated with Aβ-Induced Platelet Activation via P-Selectin. J. Clin. Med. 2020, 9, 1642.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop