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Open AccessArticle

Hsa-miR-34a-5p and hsa-miR-375 as Biomarkers for Monitoring the Effects of Drug Treatment for Migraine Pain in Children and Adolescents: A Pilot Study

1
Department of Health Sciences, University of Magna Graecia, 88100 Catanzaro CZ, Italy
2
Department of Pharmacy, Health and Nutritional Sciences, Department of Excellence 2018-2022, University of Calabria, 87036 Arcavacata, Rende CS, Italy
3
Operative Unit of Pediatric diseases, Pugliese Ciaccio Hospital, 88100 Catanzaro CZ, Italy
4
Department of Neurosurgery, University of Catanzaro, Campus Germaneto, 88100 Catanzaro CZ, Italy
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Section of Child and Adolescent Neuropsychiatry, Department of Human Neuroscience, "Sapienza" University, 00185, Rome RM, Italy
6
Dentasam, 95128 Catania CT, Italy
7
Department of Medical Science, Surgical Science and advanced Technologies “G.F, Ingrassia”, University of Catania, 95124 Catania CT, Italy
8
Forensic Medicine, Department of Law, Economy and Sociology, Magna Graecia University of Catanzaro, 88100 Catanzaro CZ, Italy
*
Authors to whom correspondence should be addressed.
Those authors equally contributed.
J. Clin. Med. 2019, 8(7), 928; https://doi.org/10.3390/jcm8070928
Received: 1 May 2019 / Revised: 17 June 2019 / Accepted: 24 June 2019 / Published: 27 June 2019
MicroRNAs (miRs) have emerged as biomarkers of migraine disease in both adults and children. In this study we evaluated the expression of hsa-miR-34a-5p and hsa-miR-375 in serum and saliva of young subjects (age 11 ± 3.467 years) with migraine without aura (MWA), while some underwent pharmacological treatment, and healthy young subjects were used as controls. miRs were determined using the qRT-PCR method, and gene targets of hsa-miR-34a-5p and hsa-miR-375 linked to pain-migraine were found by in silico analysis. qRT-PCR revealed comparable levels of hsa-miRs in both blood and saliva. Higher expression of hsa-miR-34a-5p and hsa-miR-375 was detected in saliva of untreated MWAs compared to healthy subjects (hsa-miR-34a-5p: p < 0.05; hsa-miR-375 p < 0.01). Furthermore, in MWA treated subjects, a significant decrease of hsa-miR-34a-5p and of hsa-miR-375 was documented in saliva and blood compared to MWA untreated ones. Altogether, these findings suggested thathsa-miR-34a-5p and hsa-miR-375 are expressed equally in blood and saliva and that they could be a useful biomarker of disease and of drug efficacy in patients with MWA. View Full-Text
Keywords: biomarkers; miRs; saliva; blood; pain-migraine; drug treatment; clinical risk management biomarkers; miRs; saliva; blood; pain-migraine; drug treatment; clinical risk management
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Gallelli, L.; Cione, E.; Peltrone, F.; Siviglia, S.; Verano, A.; Chirchiglia, D.; Zampogna, S.; Guidetti, V.; Sammartino, L.; Montana, A.; Caroleo, M.C.; De Sarro, G.; Di Mizio, G. Hsa-miR-34a-5p and hsa-miR-375 as Biomarkers for Monitoring the Effects of Drug Treatment for Migraine Pain in Children and Adolescents: A Pilot Study. J. Clin. Med. 2019, 8, 928.

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