Figure 1.
(a) The panoramic image and CBCT scans revealed severe vertical bone resorption along with a hopeless third molar. Additionally, a retention cyst was observed in the maxillary sinus; (b) The preoperative periapical radiograph revealed severe vertical ridge deficiency.
Figure 1.
(a) The panoramic image and CBCT scans revealed severe vertical bone resorption along with a hopeless third molar. Additionally, a retention cyst was observed in the maxillary sinus; (b) The preoperative periapical radiograph revealed severe vertical ridge deficiency.
Figure 2.
(a) Severe vertical deficiency in the right posterior maxilla; (b) The replaceable osteoinductive bony window (ROBW) was prepared using a thin-bladed saw tip connected to a piezoelectric device.; (c) Concentrated growth factor (CGF) membranes were placed beneath the elevated and perforated sinus membrane after removal of the retention cyst; (d) A rectangular wide-head tenting pole screw (WHTPS) was placed at the center of the defect to resist soft tissue contraction and support the graft; (e) Sticky autogenous tooth bone was grafted over the screw head to fill the defect. (f) A resorbable collagen barrier was placed over the graft to promote guided bone regeneration.
Figure 2.
(a) Severe vertical deficiency in the right posterior maxilla; (b) The replaceable osteoinductive bony window (ROBW) was prepared using a thin-bladed saw tip connected to a piezoelectric device.; (c) Concentrated growth factor (CGF) membranes were placed beneath the elevated and perforated sinus membrane after removal of the retention cyst; (d) A rectangular wide-head tenting pole screw (WHTPS) was placed at the center of the defect to resist soft tissue contraction and support the graft; (e) Sticky autogenous tooth bone was grafted over the screw head to fill the defect. (f) A resorbable collagen barrier was placed over the graft to promote guided bone regeneration.
Figure 3.
(a) Post-operative panoramic image of CBCT shows vertically grafted bone; (b) Cross-sectional images of CBCT reveal horizontally grafted bone; (c) Panoramic image of CBCT after 6 months of healing demonstrates that vertical alveolar ridge augmentation is distinctly observed; (d) The cross-sectional image at 6 months post-surgery demonstrates that horizontal ridge augmentation was remarkably well achieved.
Figure 3.
(a) Post-operative panoramic image of CBCT shows vertically grafted bone; (b) Cross-sectional images of CBCT reveal horizontally grafted bone; (c) Panoramic image of CBCT after 6 months of healing demonstrates that vertical alveolar ridge augmentation is distinctly observed; (d) The cross-sectional image at 6 months post-surgery demonstrates that horizontal ridge augmentation was remarkably well achieved.
Figure 4.
(a) After 6 months of healing, the WHTPS was removed, demonstrating excellent alveolar ridge regeneration and reconstruction. A bone biopsy was performed to assess tissue regeneration; (b) Three implants were placed; (c) Intraoral photographs taken 6 months after the final prosthesis placement showed stable soft tissue around the restoration (Restoration by Dr. Siwoo Lee); (d) Radiographic images taken 6 months after functional loading revealed stable crestal bone preservation above the implant platform. The letter a indicates the orientation mark of the radiograph.
Figure 4.
(a) After 6 months of healing, the WHTPS was removed, demonstrating excellent alveolar ridge regeneration and reconstruction. A bone biopsy was performed to assess tissue regeneration; (b) Three implants were placed; (c) Intraoral photographs taken 6 months after the final prosthesis placement showed stable soft tissue around the restoration (Restoration by Dr. Siwoo Lee); (d) Radiographic images taken 6 months after functional loading revealed stable crestal bone preservation above the implant platform. The letter a indicates the orientation mark of the radiograph.
Figure 5.
Hematoxylin and Eosin (H&E) staining; (a) Magnification 12.5×. The histological findings demonstrate successful graft integration, active osteogenesis, and a stable healing process. Notably, there are no signs of adverse inflammatory reactions or inflammation, indicating a favorable healing environment and effective bone regeneration (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Newly formed bone (N) is distinctly visible with well-defined osteocyte lacunae, signifying active bone formation and maturation. The residual graft material (g) is observed in close contact with the newly formed bone, indicating successful integration and demonstrating its osteoconductive properties (Scale bar: 100 μm); (c) Magnification 100×—lower panel. Newly formed bone (N) is prominently visible, characterized by well-defined osteocyte lacunae, which reflect active bone formation and maturation. The residual graft material (g) is closely associated with the newly formed bone, indicating successful integration and demonstrating its osteoconductive properties (Scale bar: 100 μm).
Figure 5.
Hematoxylin and Eosin (H&E) staining; (a) Magnification 12.5×. The histological findings demonstrate successful graft integration, active osteogenesis, and a stable healing process. Notably, there are no signs of adverse inflammatory reactions or inflammation, indicating a favorable healing environment and effective bone regeneration (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Newly formed bone (N) is distinctly visible with well-defined osteocyte lacunae, signifying active bone formation and maturation. The residual graft material (g) is observed in close contact with the newly formed bone, indicating successful integration and demonstrating its osteoconductive properties (Scale bar: 100 μm); (c) Magnification 100×—lower panel. Newly formed bone (N) is prominently visible, characterized by well-defined osteocyte lacunae, which reflect active bone formation and maturation. The residual graft material (g) is closely associated with the newly formed bone, indicating successful integration and demonstrating its osteoconductive properties (Scale bar: 100 μm).
![Jcm 14 06772 g005 Jcm 14 06772 g005]()
Figure 6.
Masson’s Trichrome (MT) stain; (a) Magnification 12.5×. The distribution of new bone (blue-stained areas) around the bone grafts (red-stained areas) suggests effective graft incorporation and active bone regeneration (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Demonstrating direct contact between newly formed bone (N) and bone grafts (g). Evidence of active osteogenesis is seen, with mineralized bone (blue) forming around the graft materials (red). The surrounding area shows well-developed connective tissue with no signs of inflammation (Scale bar: 100 μm); (c) Magnification 100×—lower panel. Clear boundaries between the mineralized new bone (N) and the graft materials (g) are observed, indicating successful remodeling and integration of the graft within the host bone (Scale bar: 100 μm).
Figure 6.
Masson’s Trichrome (MT) stain; (a) Magnification 12.5×. The distribution of new bone (blue-stained areas) around the bone grafts (red-stained areas) suggests effective graft incorporation and active bone regeneration (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Demonstrating direct contact between newly formed bone (N) and bone grafts (g). Evidence of active osteogenesis is seen, with mineralized bone (blue) forming around the graft materials (red). The surrounding area shows well-developed connective tissue with no signs of inflammation (Scale bar: 100 μm); (c) Magnification 100×—lower panel. Clear boundaries between the mineralized new bone (N) and the graft materials (g) are observed, indicating successful remodeling and integration of the graft within the host bone (Scale bar: 100 μm).
Figure 7.
(a) Severe vertical bone defects are observed in the preoperative periapical radiograph; (b) The preoperative panoramic CBCT scan images also reveal significant vertical bone defects.
Figure 7.
(a) Severe vertical bone defects are observed in the preoperative periapical radiograph; (b) The preoperative panoramic CBCT scan images also reveal significant vertical bone defects.
Figure 8.
(a) After elevating the buccal flap, a severe vertical bone defect and poor bone quality were observed in the extraction socket; (b) To achieve initial stabilization of the WHTPS, it was placed into the buccal cortical bone with good bone quality. Notably, a 6 mm vertical defect was observed; (c) Sticky conditioned bone was grafted into the defect area; (d) After covering the bone graft material with a resorbable barrier membrane, four CGF membranes were placed over the barrier membrane to promote soft tissue healing. The soft tissue was then sutured without tension.
Figure 8.
(a) After elevating the buccal flap, a severe vertical bone defect and poor bone quality were observed in the extraction socket; (b) To achieve initial stabilization of the WHTPS, it was placed into the buccal cortical bone with good bone quality. Notably, a 6 mm vertical defect was observed; (c) Sticky conditioned bone was grafted into the defect area; (d) After covering the bone graft material with a resorbable barrier membrane, four CGF membranes were placed over the barrier membrane to promote soft tissue healing. The soft tissue was then sutured without tension.
Figure 9.
(a) Vertically grafted bone is observed around the WHTPS; (b) Cross-sectional CBCT image showing vertically grafted bone; (c) After 5 months of healing, the bone builder was removed, and remarkable three-dimensional bone regeneration was observed beneath the bone builder; (d) Periapical radiograph taken after the removal of the bone builder at 5 months shows sufficient vertical ridge augmentation. The triangle indicates the orientation marker of the periapical radiograph.
Figure 9.
(a) Vertically grafted bone is observed around the WHTPS; (b) Cross-sectional CBCT image showing vertically grafted bone; (c) After 5 months of healing, the bone builder was removed, and remarkable three-dimensional bone regeneration was observed beneath the bone builder; (d) Periapical radiograph taken after the removal of the bone builder at 5 months shows sufficient vertical ridge augmentation. The triangle indicates the orientation marker of the periapical radiograph.
Figure 10.
Hematoxylin and Eosin (H&E) staining; (a) Magnification 12.5×. A 29.6% rate of new bone formation was observed, with no signs of inflammation (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. A significant amount of newly formed bone (N) is observed. Some newly formed bone is associated with a small amount of bone graft material (g), though the boundaries between them are indistinct under H&E staining. Active bone formation is prominent, with newly formed bone fragments visible. Sparse connective tissue is observed in the surrounding area (Scale bar: 100 μm); (c) Magnification 100×—lower panel. A considerable amount of newly formed bone (N) is observed in association with a small amount of allograft (g). While the new bone appears well-developed, the amount of connective tissue is insufficient compared to the newly formed bone. No signs of inflammation are present (Scale bar: 100 μm).
Figure 10.
Hematoxylin and Eosin (H&E) staining; (a) Magnification 12.5×. A 29.6% rate of new bone formation was observed, with no signs of inflammation (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. A significant amount of newly formed bone (N) is observed. Some newly formed bone is associated with a small amount of bone graft material (g), though the boundaries between them are indistinct under H&E staining. Active bone formation is prominent, with newly formed bone fragments visible. Sparse connective tissue is observed in the surrounding area (Scale bar: 100 μm); (c) Magnification 100×—lower panel. A considerable amount of newly formed bone (N) is observed in association with a small amount of allograft (g). While the new bone appears well-developed, the amount of connective tissue is insufficient compared to the newly formed bone. No signs of inflammation are present (Scale bar: 100 μm).
![Jcm 14 06772 g010 Jcm 14 06772 g010]()
Figure 11.
Masson’s Trichrome (MT) stain; (a) Magnification 12.5×. Active new bone formation (blue-stained) surrounds red-stained bone grafts, indicating robust bone regeneration (Scale bar: 500 μm). White, Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Demonstrating direct contact between newly formed bone (N) and bone grafts (g). Evidence of active osteogenesis is seen, with mineralized bone (blue) forming around the graft materials (red). The amount of connective tissue is insufficient compared to the newly formed bone. No signs of inflammation are present (Scale bar: 100 μm); (c) Magnification 100×—lower panel. The lower panel shows clear integration between the graft material (g) and newly formed bone (N). Mineralized bone tissue (blue-stained) is observed connecting seamlessly with areas of the bone grafts (red-stained), highlighting effective graft incorporation and bone remodeling (Scale bar: 100 μm).
Figure 11.
Masson’s Trichrome (MT) stain; (a) Magnification 12.5×. Active new bone formation (blue-stained) surrounds red-stained bone grafts, indicating robust bone regeneration (Scale bar: 500 μm). White, Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Demonstrating direct contact between newly formed bone (N) and bone grafts (g). Evidence of active osteogenesis is seen, with mineralized bone (blue) forming around the graft materials (red). The amount of connective tissue is insufficient compared to the newly formed bone. No signs of inflammation are present (Scale bar: 100 μm); (c) Magnification 100×—lower panel. The lower panel shows clear integration between the graft material (g) and newly formed bone (N). Mineralized bone tissue (blue-stained) is observed connecting seamlessly with areas of the bone grafts (red-stained), highlighting effective graft incorporation and bone remodeling (Scale bar: 100 μm).
![Jcm 14 06772 g011 Jcm 14 06772 g011]()
Figure 12.
(a) A plain radiograph showed severe periodontal bone loss in the second molar; (b) The cross-sectional image of the preoperative CBCT scans revealed horizontal deficiency in the premolar area; (c,d) The panoramic image and cross-sectional CBCT scans demonstrate a severe three-dimensional bone defect.
Figure 12.
(a) A plain radiograph showed severe periodontal bone loss in the second molar; (b) The cross-sectional image of the preoperative CBCT scans revealed horizontal deficiency in the premolar area; (c,d) The panoramic image and cross-sectional CBCT scans demonstrate a severe three-dimensional bone defect.
Figure 13.
(a) Preparation of the ROBW; (b) Note the significant mucosal proliferation observed during the mucosal elevation process; (c) CGF blocks were placed under the perforated sinus mucosa to enhance bone regeneration and accelerate the healing of the perforated sinus mucosa; (d) Repositioning of the ROVW to maintain sinus integrity and accelerate bone regeneration in the sinus; (e) A rectangular WHTPS was placed at the center of the defect to facilitate new bone formation; (f) A round ROBW was placed on the buccal cortex to reconstruct horizontal deficiencies in the premolar region; (g) Sticky tooth bone was grafted to the defect site to promote bone regeneration and maintain the structure of the graft; (h) A collagen barrier was placed over the graft material to protect it and facilitate guided bone regeneration.
Figure 13.
(a) Preparation of the ROBW; (b) Note the significant mucosal proliferation observed during the mucosal elevation process; (c) CGF blocks were placed under the perforated sinus mucosa to enhance bone regeneration and accelerate the healing of the perforated sinus mucosa; (d) Repositioning of the ROVW to maintain sinus integrity and accelerate bone regeneration in the sinus; (e) A rectangular WHTPS was placed at the center of the defect to facilitate new bone formation; (f) A round ROBW was placed on the buccal cortex to reconstruct horizontal deficiencies in the premolar region; (g) Sticky tooth bone was grafted to the defect site to promote bone regeneration and maintain the structure of the graft; (h) A collagen barrier was placed over the graft material to protect it and facilitate guided bone regeneration.
Figure 14.
(a) The panoramic image of the CBCT after 5 months of healing shows successful vertical ridge augmentation and maxillary sinus augmentation; (b) Remarkable horizontal ridge augmentation is observed in the premolar region; (c) The cross-sectional CBCT image demonstrates both horizontal and vertical ridge augmentation.
Figure 14.
(a) The panoramic image of the CBCT after 5 months of healing shows successful vertical ridge augmentation and maxillary sinus augmentation; (b) Remarkable horizontal ridge augmentation is observed in the premolar region; (c) The cross-sectional CBCT image demonstrates both horizontal and vertical ridge augmentation.
Figure 15.
(a) After 5 months of healing, the WHTPSs were removed, revealing excellent bone regeneration; (b) A bone biopsy was performed to confirm the formation of newly generated bone; (c) Three implants were placed; (d) A radiograph taken 6 months after functional loading showed stable alveolar bone around the implants. (restoration; Dr Siwoo Lee).
Figure 15.
(a) After 5 months of healing, the WHTPSs were removed, revealing excellent bone regeneration; (b) A bone biopsy was performed to confirm the formation of newly generated bone; (c) Three implants were placed; (d) A radiograph taken 6 months after functional loading showed stable alveolar bone around the implants. (restoration; Dr Siwoo Lee).
Figure 16.
Hematoxylin and Eosin (H&E) staining; (a) magnification 12.5×. The histological section shows newly formed bone distributed throughout the defect site (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Newly formed bone (N) is evident with well-organized lamellar structures, indicating maturation of the bone matrix. Osteocyte lacunae are clearly visible within the bone, reflecting active bone remodeling and stability. The absence of inflammatory cells suggests a healthy healing process and successful regeneration (Scale bar: 100 μm); (c) Magnification 100×—lower panel. A similar pattern of newly formed bone (N) is observed, characterized by a dense bone matrix and clearly defined osteocyte lacunae. The seamless integration of the newly formed bone into the surrounding areas suggests effective and successful bone regeneration. The surrounding connective tissue is well-developed and rich in collagen fibers, and no signs of inflammation are observed (Scale bar: 100 μm).
Figure 16.
Hematoxylin and Eosin (H&E) staining; (a) magnification 12.5×. The histological section shows newly formed bone distributed throughout the defect site (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Newly formed bone (N) is evident with well-organized lamellar structures, indicating maturation of the bone matrix. Osteocyte lacunae are clearly visible within the bone, reflecting active bone remodeling and stability. The absence of inflammatory cells suggests a healthy healing process and successful regeneration (Scale bar: 100 μm); (c) Magnification 100×—lower panel. A similar pattern of newly formed bone (N) is observed, characterized by a dense bone matrix and clearly defined osteocyte lacunae. The seamless integration of the newly formed bone into the surrounding areas suggests effective and successful bone regeneration. The surrounding connective tissue is well-developed and rich in collagen fibers, and no signs of inflammation are observed (Scale bar: 100 μm).
![Jcm 14 06772 g016 Jcm 14 06772 g016]()
Figure 17.
Masson’s Trichrome (MT) stain; (a) Magnification 12.5×. Active bone reformation is evident, with blue-stained newly formed bone surrounding red-stained osteoid tissue and graft material (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. The histologic image demonstrates areas of active bone remodeling. Blue-stained bone (mineralized tissue) surrounds the red-stained graft, showing good integration (Scale bar: 100 μm); (c) Magnification 100×—lower panel. It highlights the close interaction between newly formed bone (N, blue-stained) and graft material (g, red-stained). Active remodeling is observed, with osteoid formation and evidence of osteoblast and osteoclast activity contributing to bone maturation (Scale bar: 100 μm).
Figure 17.
Masson’s Trichrome (MT) stain; (a) Magnification 12.5×. Active bone reformation is evident, with blue-stained newly formed bone surrounding red-stained osteoid tissue and graft material (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. The histologic image demonstrates areas of active bone remodeling. Blue-stained bone (mineralized tissue) surrounds the red-stained graft, showing good integration (Scale bar: 100 μm); (c) Magnification 100×—lower panel. It highlights the close interaction between newly formed bone (N, blue-stained) and graft material (g, red-stained). Active remodeling is observed, with osteoid formation and evidence of osteoblast and osteoclast activity contributing to bone maturation (Scale bar: 100 μm).
Figure 18.
(a) Severe bone loss caused by advanced periodontal disease is observed around the first and second premolars; (b) The preoperative radiograph reveals severe vertical bone loss due to advanced periodontal disease following extraction; (c) The preoperative panoramic CBCT scan demonstrates significant vertical bone defects and extraction socket defects. The inverted letter a indicates the orientation mark of the radiograph.
Figure 18.
(a) Severe bone loss caused by advanced periodontal disease is observed around the first and second premolars; (b) The preoperative radiograph reveals severe vertical bone loss due to advanced periodontal disease following extraction; (c) The preoperative panoramic CBCT scan demonstrates significant vertical bone defects and extraction socket defects. The inverted letter a indicates the orientation mark of the radiograph.
Figure 19.
(a) Note the severe vertical defect in the extraction site after elevating the anterior flap. A 6 mm diameter round WHTPS was placed at the bony defect site to prevent the collapse of the bone graft material from the compression of the soft tissue matrix during the healing period; (b) For horizontal ridge augmentation of the narrow alveolar ridge in the mandibular posterior region, a tooth block bone graft was placed and stabilized using a mini-screw. In the maxillary posterior defect area, sticky tooth bone was grafted, extending beyond the cover screw of the WHTPS. (c) Instead of using a resorbable collagen membrane, three layers of CGF membranes were placed over the bone graft material to promote soft tissue healing. A tension-free closure was then achieved using a releasing incision and suturing the soft tissue without tension; (d) Remarkable three-dimensional ridge augmentation was observed after 5 months of healing.
Figure 19.
(a) Note the severe vertical defect in the extraction site after elevating the anterior flap. A 6 mm diameter round WHTPS was placed at the bony defect site to prevent the collapse of the bone graft material from the compression of the soft tissue matrix during the healing period; (b) For horizontal ridge augmentation of the narrow alveolar ridge in the mandibular posterior region, a tooth block bone graft was placed and stabilized using a mini-screw. In the maxillary posterior defect area, sticky tooth bone was grafted, extending beyond the cover screw of the WHTPS. (c) Instead of using a resorbable collagen membrane, three layers of CGF membranes were placed over the bone graft material to promote soft tissue healing. A tension-free closure was then achieved using a releasing incision and suturing the soft tissue without tension; (d) Remarkable three-dimensional ridge augmentation was observed after 5 months of healing.
Figure 20.
(a) A postoperative radiograph shows the grafted bone material placed over the WHTPS; (b) A cross-sectional CBCT image taken immediately after surgery reveals the grafted bone material surrounding the bone builder; (c) At 5 months postoperative, a radiograph shows that the grafted bone material remains well-maintained without collapse, extending up to the cover screw of the WHTPS; (d) Cross-sectional CBCT images at 5 months of healing demonstrate that the grafted bone material surrounding the bone builder remains intact. The inverted letter a and the triangle indicate orientation marks of the radiograph.
Figure 20.
(a) A postoperative radiograph shows the grafted bone material placed over the WHTPS; (b) A cross-sectional CBCT image taken immediately after surgery reveals the grafted bone material surrounding the bone builder; (c) At 5 months postoperative, a radiograph shows that the grafted bone material remains well-maintained without collapse, extending up to the cover screw of the WHTPS; (d) Cross-sectional CBCT images at 5 months of healing demonstrate that the grafted bone material surrounding the bone builder remains intact. The inverted letter a and the triangle indicate orientation marks of the radiograph.
Figure 21.
Hematoxylin and Eosin staining; (a) Magnification 12.5×. Histologic findings reveal active new bone formation surrounding tooth bone grafts. No inflammation is observed (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Straightforward integration of residual tooth graft material (t) with newly formed bone (N). The graft material is surrounded by mineralized new bone, indicating active bone regeneration and successful graft incorporation. The newly formed bone demonstrates visible osteocytes embedded within the bone matrix, indicating healthy bone maturation and remodeling. On the surface of the new bone, osteoblasts are prominently observed, reflecting active bone formation and deposition. Importantly, no signs of inflammation are present, suggesting a stable and favorable healing environment with no immune or inflammatory response interfering with the regenerative process. This indicates successful integration and regeneration of the graft material into functional bone tissue (Scale bar: 100 μm); (c) Magnification 100×—lower panel. The newly formed bone (N) demonstrates distinct osteocytes within the bone matrix, indicating proper maturation and integration of the bone. On the surface of the new bone, osteoblasts are visible, suggesting ongoing bone formation and remodeling. Notably, no graft material is observed in these areas, which indicates either complete resorption or integration of the graft material into the regenerated bone. Additionally, the absence of inflammatory signs confirms a stable healing process, free from adverse immune reactions. This reflects a successful regenerative outcome with functional and healthy bone tissue (Scale bar: 100 μm).
Figure 21.
Hematoxylin and Eosin staining; (a) Magnification 12.5×. Histologic findings reveal active new bone formation surrounding tooth bone grafts. No inflammation is observed (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Straightforward integration of residual tooth graft material (t) with newly formed bone (N). The graft material is surrounded by mineralized new bone, indicating active bone regeneration and successful graft incorporation. The newly formed bone demonstrates visible osteocytes embedded within the bone matrix, indicating healthy bone maturation and remodeling. On the surface of the new bone, osteoblasts are prominently observed, reflecting active bone formation and deposition. Importantly, no signs of inflammation are present, suggesting a stable and favorable healing environment with no immune or inflammatory response interfering with the regenerative process. This indicates successful integration and regeneration of the graft material into functional bone tissue (Scale bar: 100 μm); (c) Magnification 100×—lower panel. The newly formed bone (N) demonstrates distinct osteocytes within the bone matrix, indicating proper maturation and integration of the bone. On the surface of the new bone, osteoblasts are visible, suggesting ongoing bone formation and remodeling. Notably, no graft material is observed in these areas, which indicates either complete resorption or integration of the graft material into the regenerated bone. Additionally, the absence of inflammatory signs confirms a stable healing process, free from adverse immune reactions. This reflects a successful regenerative outcome with functional and healthy bone tissue (Scale bar: 100 μm).
![Jcm 14 06772 g021 Jcm 14 06772 g021]()
Figure 22.
Masson’s Trichrome (MT) stain; (a) Magnification 12.5×. The histological section shows mineralized new bone (blue), residual tooth bone graft material (red), and surrounding connective tissue. Numerous collagen fiber bundles are present, with graft material visible among them. A small amount of newly formed bone is observed surrounding the graft material (Scale bar: 500 μm). White frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. The upper panel highlights the interaction between residual tooth graft material (t) and newly formed bone (N). Evidence of bone deposition around the graft material demonstrates successful incorporation and bone regeneration (Scale bar: 100 μm); (c) Magnification 100×—lower panel. The lower panel reveals active new bone formation (N) within abundant connective tissue. Even with minimal tooth graft material (t), prominent new bone formation is observed. Newly formed bone contains distinct osteocytes, and osteoblasts are clearly visible on its surface, indicating active regeneration (Scale bar: 100 μm).
Figure 22.
Masson’s Trichrome (MT) stain; (a) Magnification 12.5×. The histological section shows mineralized new bone (blue), residual tooth bone graft material (red), and surrounding connective tissue. Numerous collagen fiber bundles are present, with graft material visible among them. A small amount of newly formed bone is observed surrounding the graft material (Scale bar: 500 μm). White frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. The upper panel highlights the interaction between residual tooth graft material (t) and newly formed bone (N). Evidence of bone deposition around the graft material demonstrates successful incorporation and bone regeneration (Scale bar: 100 μm); (c) Magnification 100×—lower panel. The lower panel reveals active new bone formation (N) within abundant connective tissue. Even with minimal tooth graft material (t), prominent new bone formation is observed. Newly formed bone contains distinct osteocytes, and osteoblasts are clearly visible on its surface, indicating active regeneration (Scale bar: 100 μm).
![Jcm 14 06772 g022 Jcm 14 06772 g022]()
Figure 23.
The preoperative panoramic radiograph reveals generalized moderate to severe alveolar bone resorption in the mandible, with particularly pronounced bone defects observed in the left posterior region.
Figure 23.
The preoperative panoramic radiograph reveals generalized moderate to severe alveolar bone resorption in the mandible, with particularly pronounced bone defects observed in the left posterior region.
Figure 24.
(a) After elevating the anterior flap, a severe vertical alveolar bone defect was observed in the left posterior region; (b) Dental implants were placed in the #33 and #34 regions, and a 6-mm diameter round WHTPS was installed at the site of the severe vertical alveolar bone defect posterior to these implants to prevent the collapse of the bone graft material caused by soft tissue pressure during the healing process; (c) A sticky bone graft material composed of a mixture of autogenous bone, allograft, and xenograft was placed around the implant sites and the WHTPS, extending beyond the head of the WHTPS; (d) A resorbable membrane was placed for GBR. Due to the sticky consistency of the graft, no additional fixation was necessary. CGF membranes were added to promote soft tissue healing.
Figure 24.
(a) After elevating the anterior flap, a severe vertical alveolar bone defect was observed in the left posterior region; (b) Dental implants were placed in the #33 and #34 regions, and a 6-mm diameter round WHTPS was installed at the site of the severe vertical alveolar bone defect posterior to these implants to prevent the collapse of the bone graft material caused by soft tissue pressure during the healing process; (c) A sticky bone graft material composed of a mixture of autogenous bone, allograft, and xenograft was placed around the implant sites and the WHTPS, extending beyond the head of the WHTPS; (d) A resorbable membrane was placed for GBR. Due to the sticky consistency of the graft, no additional fixation was necessary. CGF membranes were added to promote soft tissue healing.
Figure 25.
(a) A postoperative radiograph shows the grafted bone material placed over the WHTPS; (b) A cross-sectional CBCT image taken immediately after surgery reveals the grafted bone material surrounding the bone builder; (c) At 18 months postoperative, a radiograph shows that the grafted bone material remains well-maintained without collapse, extending up to the cover screw of the WHTPS; (d) Cross-sectional CBCT images at 18 months of healing demonstrate that the grafted bone material surrounding the bone builder remains intact. The inverted letter a indicates orientation mark of the radiograph.
Figure 25.
(a) A postoperative radiograph shows the grafted bone material placed over the WHTPS; (b) A cross-sectional CBCT image taken immediately after surgery reveals the grafted bone material surrounding the bone builder; (c) At 18 months postoperative, a radiograph shows that the grafted bone material remains well-maintained without collapse, extending up to the cover screw of the WHTPS; (d) Cross-sectional CBCT images at 18 months of healing demonstrate that the grafted bone material surrounding the bone builder remains intact. The inverted letter a indicates orientation mark of the radiograph.
Figure 26.
Hematoxylin and Eosin staining; (a) Magnification 12.5×. A 57.5% rate of new bone formation was observed, with no signs of inflammation. Overall, a well-developed bone structure is observed. A large amount of connective tissue and numerous graft materials are observed in the upper part, and new bone formation in this area is minimal. In the lower part of the well-developed mature bone, a bone marrow structure containing fat cells is observed (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Numerous graft materials (g) are observed in conjunction with well-developed bone (N). In some cases, graft material absorption by osteoclasts (arrows) is observed, and in some cases, new bone formation by osteoblasts (arrowheads) is also observed. In mature bone, numerous osteocytes are evident (Scale bar: 100 μm); (c) Magnification 100×—lower panel. A well-developed, mature bone (N) structure is observed. The amount of connective tissue is minimal, but in some cases, new bone formation by osteoblasts (arrowheads) is observed (Scale bar: 100 μm).
Figure 26.
Hematoxylin and Eosin staining; (a) Magnification 12.5×. A 57.5% rate of new bone formation was observed, with no signs of inflammation. Overall, a well-developed bone structure is observed. A large amount of connective tissue and numerous graft materials are observed in the upper part, and new bone formation in this area is minimal. In the lower part of the well-developed mature bone, a bone marrow structure containing fat cells is observed (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. Numerous graft materials (g) are observed in conjunction with well-developed bone (N). In some cases, graft material absorption by osteoclasts (arrows) is observed, and in some cases, new bone formation by osteoblasts (arrowheads) is also observed. In mature bone, numerous osteocytes are evident (Scale bar: 100 μm); (c) Magnification 100×—lower panel. A well-developed, mature bone (N) structure is observed. The amount of connective tissue is minimal, but in some cases, new bone formation by osteoblasts (arrowheads) is observed (Scale bar: 100 μm).
![Jcm 14 06772 g026 Jcm 14 06772 g026]()
Figure 27.
Masson’s Trichrome (MT) stain; (a) Magnification 12.5×. The structure of well-developed bone is observed. As a result of the MT stain, new bone (blue) and mature bone or lamellar bone (red) are observed separately (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. The absorption of graft materials is observed between well-developed bones (N). Many osteoclasts (arrows) are observed in the connective tissue around the graft material (g). Most of the osteoclasts are located at the border with the graft material, showing active absorption of the graft material. Osteocytes are distinct in mature bone (Scale bar: 100 μm); (c) Magnification 100×—lower panel. Lamellar bone (asterisk) is distinct in the structure of well-developed bone (N). The bone matrix of mature bone contains distinct osteocytes, and osteoblasts are in small proportions. The absorption of graft materials (g) by osteoclasts (arrows) is observed in the connective tissue between the bones (Scale bar: 100 μm).
Figure 27.
Masson’s Trichrome (MT) stain; (a) Magnification 12.5×. The structure of well-developed bone is observed. As a result of the MT stain, new bone (blue) and mature bone or lamellar bone (red) are observed separately (Scale bar: 500 μm). Black frames indicate the region of interest selected for higher magnification views in panels (b,c); (b) Magnification 100×—upper panel. The absorption of graft materials is observed between well-developed bones (N). Many osteoclasts (arrows) are observed in the connective tissue around the graft material (g). Most of the osteoclasts are located at the border with the graft material, showing active absorption of the graft material. Osteocytes are distinct in mature bone (Scale bar: 100 μm); (c) Magnification 100×—lower panel. Lamellar bone (asterisk) is distinct in the structure of well-developed bone (N). The bone matrix of mature bone contains distinct osteocytes, and osteoblasts are in small proportions. The absorption of graft materials (g) by osteoclasts (arrows) is observed in the connective tissue between the bones (Scale bar: 100 μm).
![Jcm 14 06772 g027 Jcm 14 06772 g027]()
Table 1.
Summary of vertical ridge augmentation outcomes using WHTPSs in five clinical cases.
Table 1.
Summary of vertical ridge augmentation outcomes using WHTPSs in five clinical cases.
Case No. | Location | Augmentation Height (mm) | Bone Formation (%) | Healing Duration Before Biopsy (Month) | Type of Graft Used |
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1 | Maxilla Right | 10 mm | 21.2% | 6 | Autogenous tooth bone |
2 | Maxilla Left | 6 mm | 29.6% | 5 | Allograft + Xenograft |
3 | Maxilla Left | 10 mm | 32.0% | 5 | Allograft + Xenograft |
4 | Mandible Left | 9 mm | 35.9% | 5 | Autogenous tooth bone |
5 | Mandible Right | 8 mm | 57.5% | 18 | Autograft + Allograft + Xenograft |