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Article

Detection of Alpha- and Betacoronaviruses in Miniopterus fuliginosus and Rousettus leschenaultii, two species of Sri Lankan Bats

1
Robert Koch Institute, Centre for Biological Threats and Special Pathogens, Highly Pathogenic Viruses (ZBS 1), 13353 Berlin, Germany
2
Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo 00300, Sri Lanka
3
IDEA (Identification of Emerging Agents) Laboratory, Department of Zoology and Environment Sciences, University of Colombo, Colombo 00300, Sri Lanka
4
Robert Koch Institute, Centre for International Health Protection, Public Health Laboratory Support (ZIG 4), 13353 Berlin, Germany
5
Colombo North Teaching Hospital, Ragama 11010, Sri Lanka
*
Author to whom correspondence should be addressed.
Academic Editors: Davide Lelli and Ana Moreno
Vaccines 2021, 9(6), 650; https://doi.org/10.3390/vaccines9060650
Received: 12 May 2021 / Revised: 3 June 2021 / Accepted: 9 June 2021 / Published: 15 June 2021
(This article belongs to the Special Issue Research in Bat-Borne Zoonotic Viruses)
Bats are known to be potential reservoirs of numerous human-pathogenic viruses. They have been identified as natural hosts for coronaviruses, causing Severe Acute Respiratory Syndrome (SARS) in humans. Since the emergence of SARS-CoV-2 in 2019 interest in the prevalence of coronaviruses in bats was newly raised. In this study we investigated different bat species living in a sympatric colony in the Wavul Galge cave (Koslanda, Sri Lanka). In three field sessions (in 2018 and 2019), 395 bats were captured (Miniopterus, Rousettus, Hipposideros and Rhinolophus spp.) and either rectal swabs or fecal samples were collected. From these overall 396 rectal swab and fecal samples, the screening for coronaviruses with nested PCR resulted in 33 positive samples, 31 of which originated from Miniopterus fuliginosus and two from Rousettus leschenaultii. Sanger sequencing and phylogenetic analysis of the obtained 384-nt fragment of the RNA-dependent RNA polymerase revealed that the examined M. fuliginosus bats excrete alphacoronaviruses and the examined R. leschenaultii bats excrete betacoronaviruses. Despite the sympatric roosting habitat, the coronaviruses showed host specificity and seemed to be limited to one species. Our results represent an important basis to better understand the prevalence of coronaviruses in Sri Lankan bats and may provide a basis for pursuing studies on particular bat species of interest. View Full-Text
Keywords: bat coronavirus; Miniopterus fuliginosus; Rousettus leschenaultii; Sri Lanka; cave-dwelling; sympatric colony; alphacoronavirus; betacoronavirus bat coronavirus; Miniopterus fuliginosus; Rousettus leschenaultii; Sri Lanka; cave-dwelling; sympatric colony; alphacoronavirus; betacoronavirus
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MDPI and ACS Style

Muzeniek, T.; Perera, T.; Siriwardana, S.; Bas, D.; Kaplan, F.; Öruc, M.; Becker-Ziaja, B.; Schwarz, F.; Premawansa, G.; Premawansa, S.; Perera, I.; Yapa, W.; Nitsche, A.; Kohl, C. Detection of Alpha- and Betacoronaviruses in Miniopterus fuliginosus and Rousettus leschenaultii, two species of Sri Lankan Bats. Vaccines 2021, 9, 650. https://doi.org/10.3390/vaccines9060650

AMA Style

Muzeniek T, Perera T, Siriwardana S, Bas D, Kaplan F, Öruc M, Becker-Ziaja B, Schwarz F, Premawansa G, Premawansa S, Perera I, Yapa W, Nitsche A, Kohl C. Detection of Alpha- and Betacoronaviruses in Miniopterus fuliginosus and Rousettus leschenaultii, two species of Sri Lankan Bats. Vaccines. 2021; 9(6):650. https://doi.org/10.3390/vaccines9060650

Chicago/Turabian Style

Muzeniek, Therese, Thejanee Perera, Sahan Siriwardana, Dilara Bas, Fatimanur Kaplan, Mizgin Öruc, Beate Becker-Ziaja, Franziska Schwarz, Gayani Premawansa, Sunil Premawansa, Inoka Perera, Wipula Yapa, Andreas Nitsche, and Claudia Kohl. 2021. "Detection of Alpha- and Betacoronaviruses in Miniopterus fuliginosus and Rousettus leschenaultii, two species of Sri Lankan Bats" Vaccines 9, no. 6: 650. https://doi.org/10.3390/vaccines9060650

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