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Article

Reduced Virus Load in Lungs of Pigs Challenged with Porcine Reproductive and Respiratory Syndrome Virus after Vaccination with Virus Replicon Particles Encoding Conserved PRRSV Cytotoxic T-Cell Epitopes

1
Section for Veterinary Clinical Microbiology, Department of Veterinary and Animal Sciences, University of Copenhagen, Dyrlægevej 88, 1870 Frederiksberg C, Denmark
2
Institute of Virology and Immunology IVI, Sensemattstrasse 293, 3147 Mittelhäusern, Switzerland
3
Department of Infectious Diseases and Pathobiology (DIP), Vetsuisse Faculty, University of Bern, Länggassstrasse 120, 3012 Bern, Switzerland
4
Center for Vaccine Research, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark
*
Author to whom correspondence should be addressed.
Academic Editor: Daniel Dory
Vaccines 2021, 9(3), 208; https://doi.org/10.3390/vaccines9030208
Received: 18 January 2021 / Revised: 25 February 2021 / Accepted: 25 February 2021 / Published: 2 March 2021
(This article belongs to the Special Issue Reverse Vaccinology and Genomics)
Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe respiratory distress and reproductive failure in swine. Modified live virus (MLV) vaccines provide the highest degree of protection and are most often the preferred choice. While somewhat protective, the use of MLVs is accompanied by multiple safety issues, why safer alternatives are urgently needed. Here, we describe the generation of virus replicon particles (VRPs) based on a classical swine fever virus genome incapable of producing infectious progeny and designed to express conserved PRRSV-2 cytotoxic T-cell epitopes. Eighteen pigs matched with the epitopes by their swine leucocyte antigen-profiles were vaccinated (N = 11, test group) or sham-vaccinated (N = 7, control group) with the VRPs and subsequently challenged with PRRSV-2. The responses to vaccination and challenge were monitored using serological, immunological, and virological analyses. Challenge virus load in serum did not differ significantly between the groups, whereas the virus load in the caudal part of the lung was significantly lower in the test group compared to the control group. The number of peptide-induced interferon-γ secreting cells after challenge was higher and more frequent in the test group than in the control group. Together, our results provide indications of a shapeable PRRSV-specific cell-mediated immune response that may inspire future development of effective PRRSV vaccines. View Full-Text
Keywords: porcine reproductive and respiratory syndrome virus (PRRSV); virus replicon particles (VRP); classical swine fever virus (CSFV); viral vector; vaccine; polyepitope antigen; cytotoxic T cells; cell-mediated immunity porcine reproductive and respiratory syndrome virus (PRRSV); virus replicon particles (VRP); classical swine fever virus (CSFV); viral vector; vaccine; polyepitope antigen; cytotoxic T cells; cell-mediated immunity
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MDPI and ACS Style

Welner, S.; Ruggli, N.; Liniger, M.; Summerfield, A.; Larsen, L.E.; Jungersen, G. Reduced Virus Load in Lungs of Pigs Challenged with Porcine Reproductive and Respiratory Syndrome Virus after Vaccination with Virus Replicon Particles Encoding Conserved PRRSV Cytotoxic T-Cell Epitopes. Vaccines 2021, 9, 208. https://doi.org/10.3390/vaccines9030208

AMA Style

Welner S, Ruggli N, Liniger M, Summerfield A, Larsen LE, Jungersen G. Reduced Virus Load in Lungs of Pigs Challenged with Porcine Reproductive and Respiratory Syndrome Virus after Vaccination with Virus Replicon Particles Encoding Conserved PRRSV Cytotoxic T-Cell Epitopes. Vaccines. 2021; 9(3):208. https://doi.org/10.3390/vaccines9030208

Chicago/Turabian Style

Welner, Simon, Nicolas Ruggli, Matthias Liniger, Artur Summerfield, Lars E. Larsen, and Gregers Jungersen. 2021. "Reduced Virus Load in Lungs of Pigs Challenged with Porcine Reproductive and Respiratory Syndrome Virus after Vaccination with Virus Replicon Particles Encoding Conserved PRRSV Cytotoxic T-Cell Epitopes" Vaccines 9, no. 3: 208. https://doi.org/10.3390/vaccines9030208

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