CD4+ T Cells Induced by Tuberculosis Subunit Vaccine H1 Can Improve the HIV-1 Env Humoral Response by Intrastructural Help
Abstract
1. Introduction
2. Materials and Methods
2.1. DNA Plasmids
2.2. Cell Culture
2.3. VLP Purification
2.4. VLP Quantification and Analysis
2.5. Mouse Housing and Ethic Statements
2.6. Immunization
2.7. Analysis of Cellular Immune Response
2.8. Analysis of the Humoral Immune Response
2.9. In Vitro T Cell Activation
2.10. Statistical Analysis
3. Results
3.1. Comparison of T Cell Responses Induced by H1/CAF01 or H1-DNA Immunization
3.2. Generation of Peptide-Bearing VLPs
3.3. Analysis of VLP Uptake, Processing, and Peptide Presentation
3.4. H1 Priming Immunization Enhances Env IgG Response by Intrastructural Help
3.5. H1/CAF01 Immunization Can Modulate Env-Specific IgG Isotypes
3.6. H1/CAF01 Priming Enhances Frequency of Long-Lived Plasma Cells
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A
References
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Klessing, S.; Temchura, V.; Tannig, P.; Peter, A.S.; Christensen, D.; Lang, R.; Überla, K. CD4+ T Cells Induced by Tuberculosis Subunit Vaccine H1 Can Improve the HIV-1 Env Humoral Response by Intrastructural Help. Vaccines 2020, 8, 604. https://doi.org/10.3390/vaccines8040604
Klessing S, Temchura V, Tannig P, Peter AS, Christensen D, Lang R, Überla K. CD4+ T Cells Induced by Tuberculosis Subunit Vaccine H1 Can Improve the HIV-1 Env Humoral Response by Intrastructural Help. Vaccines. 2020; 8(4):604. https://doi.org/10.3390/vaccines8040604
Chicago/Turabian StyleKlessing, Stephan, Vladimir Temchura, Pierre Tannig, Antonia Sophia Peter, Dennis Christensen, Roland Lang, and Klaus Überla. 2020. "CD4+ T Cells Induced by Tuberculosis Subunit Vaccine H1 Can Improve the HIV-1 Env Humoral Response by Intrastructural Help" Vaccines 8, no. 4: 604. https://doi.org/10.3390/vaccines8040604
APA StyleKlessing, S., Temchura, V., Tannig, P., Peter, A. S., Christensen, D., Lang, R., & Überla, K. (2020). CD4+ T Cells Induced by Tuberculosis Subunit Vaccine H1 Can Improve the HIV-1 Env Humoral Response by Intrastructural Help. Vaccines, 8(4), 604. https://doi.org/10.3390/vaccines8040604