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Open AccessArticle

CD4+ T Cells Induced by Tuberculosis Subunit Vaccine H1 Can Improve the HIV-1 Env Humoral Response by Intrastructural Help

1
Institute of Clinical and Molecular Virology, Friedrich-Alexander-University Erlangen-Nürnberg, 91054 Erlangen, Germany
2
Adjuvant Research, Dept. of Infectious Disease Immunology, Statens Serum Institut, 2300 Copenhagen, Denmark
3
Institute for Clinical Microbiology, Immunology and Hygiene, University Hospital Erlangen, Wasserturmstraße 3-5, 91054 Erlangen, Germany
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(4), 604; https://doi.org/10.3390/vaccines8040604
Received: 28 August 2020 / Revised: 1 October 2020 / Accepted: 6 October 2020 / Published: 13 October 2020
(This article belongs to the Special Issue HIV Vaccine)
The induction of a potent and long-lasting, broadly neutralizing antibody response is one of the most promising approaches in HIV-1 vaccination. Recently, we demonstrated that Gag-specific T helper cells induced by DNA priming can enhance and modulate the HIV Env-specific B cell response upon virus-like particle (VLP) boost by intrastructural help (ISH). In order to minimize the induction of potentially harmful HIV specific TH cells, we explored the possibility to harness the heterologous TH cells induced by a recombinant tuberculosis subunit vaccine H1, which contains a fusion protein of Ag85B and ESAT-6 antigens in combination with the liposomal adjuvant CAF01. To provide ISH, immunodominant MHC-II restricted peptides from the H1 vaccine were genetically incorporated into the HIV 1 Gag protein and used for HIV VLP production. ISH effects on Env-specific antibody levels and B cell differentiation were analyzed in mice primed against H1 and boosted with VLPs. In contrast to non-primed mice, a significant increase of Env-specific IgG levels for up to 26 weeks after the last immunization was observed. This increase was largely caused by elevated IgG2b and IgG2c levels in mice that received H1 priming. Additionally, ISH enhanced the frequency of Env-specific long-lived plasma cells in the bone marrow. In this study, we were able to demonstrate that a heterologous prime-boost regimen consisting of the H1 tuberculosis subunit vaccine and T helper epitope modified HIV-1 VLPs resulted in enhanced HIV Env antibody and B cell responses, mediated by intrastructural help. View Full-Text
Keywords: HIV-1 vaccine; intrastructural help; H1 tuberculosis vaccine; IgG isotype modulation HIV-1 vaccine; intrastructural help; H1 tuberculosis vaccine; IgG isotype modulation
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MDPI and ACS Style

Klessing, S.; Temchura, V.; Tannig, P.; Peter, A.S.; Christensen, D.; Lang, R.; Überla, K. CD4+ T Cells Induced by Tuberculosis Subunit Vaccine H1 Can Improve the HIV-1 Env Humoral Response by Intrastructural Help. Vaccines 2020, 8, 604. https://doi.org/10.3390/vaccines8040604

AMA Style

Klessing S, Temchura V, Tannig P, Peter AS, Christensen D, Lang R, Überla K. CD4+ T Cells Induced by Tuberculosis Subunit Vaccine H1 Can Improve the HIV-1 Env Humoral Response by Intrastructural Help. Vaccines. 2020; 8(4):604. https://doi.org/10.3390/vaccines8040604

Chicago/Turabian Style

Klessing, Stephan; Temchura, Vladimir; Tannig, Pierre; Peter, Antonia S.; Christensen, Dennis; Lang, Roland; Überla, Klaus. 2020. "CD4+ T Cells Induced by Tuberculosis Subunit Vaccine H1 Can Improve the HIV-1 Env Humoral Response by Intrastructural Help" Vaccines 8, no. 4: 604. https://doi.org/10.3390/vaccines8040604

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