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Volume 8
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10.3390/vaccines8030448
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Article

Immunogenicity and Protective Efficacy of Influenza A DNA Vaccines Encoding Artificial Antigens Based on Conservative Hemagglutinin Stem Region and M2 Protein in Mice

by
Sergei Bazhan
1,*,
Denis Antonets
1,
Ekaterina Starostina
2,
Tatyana Ilyicheva
3,
Olga Kaplina
2,
Vasiliy Marchenko
3,
Alexander Durymanov
3,
Svetlana Oreshkova
2 and
Larisa Karpenko
2
1
Theoretical Department, State Research Center of Virology and Biotechnology “Vector”, Koltsovo 630559, Novosibirsk Region, Russia
2
Bioengineering Department, State Research Center of Virology and Biotechnology “Vector”, Koltsovo 630559, Novosibirsk Region, Russia
3
Department of Zoonotic Infections and Influenza, State Research Center of Virology and Biotechnology “Vector”, Koltsovo 630559, Novosibirsk Region, Russia
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(3), 448; https://doi.org/10.3390/vaccines8030448
Submission received: 8 July 2020 / Revised: 4 August 2020 / Accepted: 6 August 2020 / Published: 9 August 2020
(This article belongs to the Special Issue Influenza Virus and Vaccine Development)
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Abstract

Background: Development of a universal vaccine capable to induce antibody responses against a broad range of influenza virus strains attracts growing attention. Hemagglutinin stem and the exposed fragment of influenza virus M2 protein are promising targets for induction of cross-protective humoral and cell-mediated response, since they contain conservative epitopes capable to induce antibodies and cytotoxic T lymphocytes (CTLs) to a wide range of influenza virus subtypes. Methods: In this study, we generated DNA vaccine constructs encoding artificial antigens AgH1, AgH3, and AgM2 designed on the basis of conservative hemagglutinin stem fragments of two influenza A virus subtypes, H1N1 and H3N2, and conservative M2 protein, and evaluate their immunogenicity and protective efficacy. To obtain DNA vaccine constructs, genes encoding the designed antigens were cloned into a pcDNA3.1 vector. Expression of the target genes in 293T cells transfected with DNA vaccine constructs has been confirmed by synthesis of specific mRNA. Results: Immunization of BALB/c mice with DNA vaccines encoding these antigens was shown to evoke humoral and T-cell immune responses as well as a moderated statistically significant cross-protective effect against two heterologous viruses A/California/4/2009 (H1N1pdm09) and A/Aichi/2/68 (H3N2). Conclusions: The results demonstrate a potential approach to creating a universal influenza vaccine based on artificial antigens.
Keywords: influenza virus; DNA-vaccine constructs; artificial polyepitope T-cell immunogens; hemagglutinin stem region; M2 protein; cross-protective influenza immunity influenza virus; DNA-vaccine constructs; artificial polyepitope T-cell immunogens; hemagglutinin stem region; M2 protein; cross-protective influenza immunity
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MDPI and ACS Style

Bazhan, S.; Antonets, D.; Starostina, E.; Ilyicheva, T.; Kaplina, O.; Marchenko, V.; Durymanov, A.; Oreshkova, S.; Karpenko, L. Immunogenicity and Protective Efficacy of Influenza A DNA Vaccines Encoding Artificial Antigens Based on Conservative Hemagglutinin Stem Region and M2 Protein in Mice. Vaccines 2020, 8, 448. https://doi.org/10.3390/vaccines8030448

AMA Style

Bazhan S, Antonets D, Starostina E, Ilyicheva T, Kaplina O, Marchenko V, Durymanov A, Oreshkova S, Karpenko L. Immunogenicity and Protective Efficacy of Influenza A DNA Vaccines Encoding Artificial Antigens Based on Conservative Hemagglutinin Stem Region and M2 Protein in Mice. Vaccines. 2020; 8(3):448. https://doi.org/10.3390/vaccines8030448

Chicago/Turabian Style

Bazhan, Sergei, Denis Antonets, Ekaterina Starostina, Tatyana Ilyicheva, Olga Kaplina, Vasiliy Marchenko, Alexander Durymanov, Svetlana Oreshkova, and Larisa Karpenko. 2020. "Immunogenicity and Protective Efficacy of Influenza A DNA Vaccines Encoding Artificial Antigens Based on Conservative Hemagglutinin Stem Region and M2 Protein in Mice" Vaccines 8, no. 3: 448. https://doi.org/10.3390/vaccines8030448

APA Style

Bazhan, S., Antonets, D., Starostina, E., Ilyicheva, T., Kaplina, O., Marchenko, V., Durymanov, A., Oreshkova, S., & Karpenko, L. (2020). Immunogenicity and Protective Efficacy of Influenza A DNA Vaccines Encoding Artificial Antigens Based on Conservative Hemagglutinin Stem Region and M2 Protein in Mice. Vaccines, 8(3), 448. https://doi.org/10.3390/vaccines8030448

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