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Vaccines
Volume 8
Issue 2
10.3390/vaccines8020260
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Article

Specificity of CD8+ T-Cell Responses Following Vaccination with Conserved Regions of HIV-1 in Nairobi, Kenya

by
Yehia S. Mohamed
1,2,
Nicola J. Borthwick
1,
Nathifa Moyo
1,
Hayato Murakoshi
3,
Tomohiro Akahoshi
3,
Francesca Siliquini
1,
Zara Hannoun
1,
Alison Crook
1,
Peter Hayes
4,
Patricia E. Fast
5,
Gaudensia Mutua
6,
Walter Jaoko
6,
Sandra Silva-Arrieta
7,
Anuska Llano
7,
Christian Brander
7,8,9,
Masafumi Takiguchi
3 and
Tomáš Hanke
1,3,*
1
The Jenner Institute, University of Oxford, Oxford OX3 7DQ, UK
2
Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo 11823, Egypt
3
Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto 860-0811, Japan
4
International AIDS Vaccine Initiative IAVI-Human Immunology Laboratory, Imperial College London, London SW10 9NH, UK
5
International AIDS Vaccine Initiative-New York, New York, NY 10004, USA
6
KAVI-Institute of Clinical Research, University of Nairobi, Nairobi 19676 00202, Kenya
7
IrsiCaixa AIDS Research Institute-HIVACAT, Hospital Universitari Germans Trias i Pujol, 08916 Barcelona, Spain
8
Faculty of Medicine, Universitat de Vic-Central de Catalunya (UVic-UCC), 08500 Vic, Spain
9
Catalan Institution for Research and Advanced Studies (ICREA), 08010 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(2), 260; https://doi.org/10.3390/vaccines8020260
Received: 4 May 2020 / Revised: 20 May 2020 / Accepted: 25 May 2020 / Published: 29 May 2020
(This article belongs to the Section HIV Vaccines)
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Abstract

Sub-Saharan Africa carries the biggest burden of the human immunodeficiency virus type 1 (HIV-1)/AIDS epidemic and is in an urgent need of an effective vaccine. CD8+ T cells are an important component of the host immune response to HIV-1 and may need to be harnessed if a vaccine is to be effective. CD8+ T cells recognize human leukocyte antigen (HLA)-associated viral epitopes and the HLA alleles vary significantly among different ethnic groups. It follows that definition of HIV-1-derived peptides recognized by CD8+ T cells in the geographically relevant regions will critically guide vaccine development. Here, we study fine details of CD8+ T-cell responses elicited in HIV-1/2-uninfected individuals in Nairobi, Kenya, who received a candidate vaccine delivering conserved regions of HIV-1 proteins called HIVconsv. Using 10-day cell lines established by in vitro peptide restimulation of cryopreserved PBMC and stably HLA-transfected 721.221/C1R cell lines, we confirm experimentally many already defined epitopes, for a number of epitopes we define the restricting HLA molecule(s) and describe four novel HLA-epitope pairs. We also identify specific dominance patterns, a promiscuous T-cell epitope and a rescue of suboptimal T-cell epitope induction in vivo by its functional variant, which all together inform vaccine design. View Full-Text
Keywords: HIV vaccine; HIVconsv; conserved regions; CD8 epitopes; HLA class I epitopes; T cell vaccine; African HLA HIV vaccine; HIVconsv; conserved regions; CD8 epitopes; HLA class I epitopes; T cell vaccine; African HLA
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MDPI and ACS Style

Mohamed, Y.S.; Borthwick, N.J.; Moyo, N.; Murakoshi, H.; Akahoshi, T.; Siliquini, F.; Hannoun, Z.; Crook, A.; Hayes, P.; Fast, P.E.; Mutua, G.; Jaoko, W.; Silva-Arrieta, S.; Llano, A.; Brander, C.; Takiguchi, M.; Hanke, T. Specificity of CD8+ T-Cell Responses Following Vaccination with Conserved Regions of HIV-1 in Nairobi, Kenya. Vaccines 2020, 8, 260. https://doi.org/10.3390/vaccines8020260

AMA Style

Mohamed YS, Borthwick NJ, Moyo N, Murakoshi H, Akahoshi T, Siliquini F, Hannoun Z, Crook A, Hayes P, Fast PE, Mutua G, Jaoko W, Silva-Arrieta S, Llano A, Brander C, Takiguchi M, Hanke T. Specificity of CD8+ T-Cell Responses Following Vaccination with Conserved Regions of HIV-1 in Nairobi, Kenya. Vaccines. 2020; 8(2):260. https://doi.org/10.3390/vaccines8020260

Chicago/Turabian Style

Mohamed, Yehia S., Nicola J. Borthwick, Nathifa Moyo, Hayato Murakoshi, Tomohiro Akahoshi, Francesca Siliquini, Zara Hannoun, Alison Crook, Peter Hayes, Patricia E. Fast, Gaudensia Mutua, Walter Jaoko, Sandra Silva-Arrieta, Anuska Llano, Christian Brander, Masafumi Takiguchi, and Tomáš Hanke. 2020. "Specificity of CD8+ T-Cell Responses Following Vaccination with Conserved Regions of HIV-1 in Nairobi, Kenya" Vaccines 8, no. 2: 260. https://doi.org/10.3390/vaccines8020260

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