Next Article in Journal
Vaccine-Induced Adverse Effects in Cultured Neuroblastoma 2A (N2A) Cells Duplicate Toxicity of Serum from Patients with Gulf War Illness (GWI) and Are Prevented in the Presence of Specific Anti-Vaccine Antibodies
Previous Article in Journal
Herpes Simplex Viruses Whose Replication Can Be Deliberately Controlled as Candidate Vaccines
vaccines-logo
Article Menu
Article

A Single Shot Pre-fusion-Stabilized Bovine RSV F Vaccine is Safe and Effective in Newborn Calves with Maternally Derived Antibodies

1
University Paris-Saclay, INRAE, UVSQ, VIM, 78350 Jouy-en-Josas, France
2
Host Pathogen Interaction Group, Unit of ruminant medicine, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Box 7054, 75007 Uppsala, Sweden
3
The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey GU24 0NF, UK
4
INRAE, PFIE, 37380 Nouzilly, France
5
INRAE, University of Tours, ISP, 37380 Nouzilly, France
6
University Paris Saclay, INRAE, AgroParisTech, GABI, 78350 Jouy-en-Josas, France
7
Department of Chemistry-BMC, Uppsala University, 875007 Uppsala, Sweden
8
Department of Biomedicine and veterinary public Health, Swedish University of Agricultural Sciences, Box 7054, SE-756 51, 875007 Uppsala, Sweden
9
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
10
Applied Immunology and Genetics, S.L. (INGENASA), 28037 Madrid, Spain
*
Author to whom correspondence should be addressed.
Vaccines 2020, 8(2), 231; https://doi.org/10.3390/vaccines8020231
Received: 25 April 2020 / Revised: 11 May 2020 / Accepted: 11 May 2020 / Published: 18 May 2020
(This article belongs to the Section Vaccines against Infectious Diseases)
Achieving safe and protective vaccination against respiratory syncytial virus (RSV) in infants and in calves has proven a challenging task. The design of recombinant antigens with a conformation close to their native form in virus particles is a major breakthrough. We compared two subunit vaccines, the bovine RSV (BRSV) pre-fusion F (preF) alone or with nanorings formed by the RSV nucleoprotein (preF+N). PreF and N proteins are potent antigenic targets for neutralizing antibodies and T cell responses, respectively. To tackle the challenges of neonatal immunization, three groups of six one-month-old calves with maternally derived serum antibodies (MDA) to BRSV received a single intramuscular injection of PreF, preF+N with MontanideTM ISA61 VG (ISA61) as adjuvant or only ISA61 (control). One month later, all calves were challenged with BRSV and monitored for virus replication in the upper respiratory tract and for clinical signs of disease over one week, and then post-mortem examinations of their lungs were performed. Both preF and preF+N vaccines afforded safe, clinical, and virological protection against BRSV, with little difference between the two subunit vaccines. Analysis of immune parameters pointed to neutralizing antibodies and antibodies to preF as being significant correlates of protection. Thus, a single shot vaccination with preF appears sufficient to reduce the burden of BRSV disease in calves with MDA. View Full-Text
Keywords: bovine; neonate; pre-fusion conformation; respiratory syncytial virus; subunit vaccine bovine; neonate; pre-fusion conformation; respiratory syncytial virus; subunit vaccine
Show Figures

Figure 1

MDPI and ACS Style

Riffault, S.; Hägglund, S.; Guzman, E.; Näslund, K.; Jouneau, L.; Dubuquoy, C.; Pietralunga, V.; Laubreton, D.; Boulesteix, O.; Gauthier, D.; Remot, A.; Boukaridi, A.; Falk, A.; Shevchenko, G.; Lind, S.B.; Vargmar, K.; Zhang, B.; Kwong, P.D.; Rodriguez, M.J.; Duran, M.G.; Schwartz-Cornil, I.; Eléouët, J.-F.; Taylor, G.; Valarcher, J.F. A Single Shot Pre-fusion-Stabilized Bovine RSV F Vaccine is Safe and Effective in Newborn Calves with Maternally Derived Antibodies. Vaccines 2020, 8, 231. https://doi.org/10.3390/vaccines8020231

AMA Style

Riffault S, Hägglund S, Guzman E, Näslund K, Jouneau L, Dubuquoy C, Pietralunga V, Laubreton D, Boulesteix O, Gauthier D, Remot A, Boukaridi A, Falk A, Shevchenko G, Lind SB, Vargmar K, Zhang B, Kwong PD, Rodriguez MJ, Duran MG, Schwartz-Cornil I, Eléouët J-F, Taylor G, Valarcher JF. A Single Shot Pre-fusion-Stabilized Bovine RSV F Vaccine is Safe and Effective in Newborn Calves with Maternally Derived Antibodies. Vaccines. 2020; 8(2):231. https://doi.org/10.3390/vaccines8020231

Chicago/Turabian Style

Riffault, Sabine, Sara Hägglund, Efrain Guzman, Katarina Näslund, Luc Jouneau, Catherine Dubuquoy, Vincent Pietralunga, Daphné Laubreton, Olivier Boulesteix, David Gauthier, Aude Remot, Abdelhak Boukaridi, Alexander Falk, Ganna Shevchenko, Sara B. Lind, Karin Vargmar, Baoshan Zhang, Peter D. Kwong, María J. Rodriguez, Marga G. Duran, Isabelle Schwartz-Cornil, Jean-François Eléouët, Geraldine Taylor, and Jean F. Valarcher 2020. "A Single Shot Pre-fusion-Stabilized Bovine RSV F Vaccine is Safe and Effective in Newborn Calves with Maternally Derived Antibodies" Vaccines 8, no. 2: 231. https://doi.org/10.3390/vaccines8020231

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop