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Vaccines 2019, 7(2), 36; https://doi.org/10.3390/vaccines7020036

New Promising Targets for Synthetic Omptin-Based Peptide Vaccine against Gram-Negative Pathogens

1
Laboratory for Molecular Biology and NanoBiotechnology, Federal Research Center for Virology and Microbiology, Branch in Saratov, 410028 Saratov, Russia
2
Department of Vaccine Control, Scientific Center on Expertise of Medical Application Products, 127051, Moscow, Russia
3
Department for Medical Optics, Saratov State University, 410012, Saratov, Russia
4
Department of Pathology, Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA
*
Authors to whom correspondence should be addressed.
Received: 25 February 2019 / Revised: 24 March 2019 / Accepted: 4 April 2019 / Published: 10 April 2019
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Abstract

Omptins represent a family of proteases commonly found in various Gram-negative pathogens. These proteins play an important role in host–pathogen interaction and have been recognized as key virulence factors, highlighting the possibility of developing an omptin-based broad-spectrum vaccine. The prototypical omptin, His-tagged recombinant Pla, was used as a model target antigen. In total, 46 linear and 24 conformational epitopes for the omptin family were predicted by the use of ElliPro service. Among these we selected highly conserved, antigenic, non-allergenic, and immunogenic B-cell epitopes. Five epitopes (2, 6, 8, 10, and 11 corresponding to Pla regions 52–60, 146–150, 231–234, 286–295, and 306–311, respectively) could be the first choice for the development of the new generation of target-peptide-based vaccine against plague. The partial residues of omptin epitopes 6, 8, and 10 (regions 136–145, 227–230, and 274–285) could be promising targets for the multi-pathogen vaccine against a group of enterobacterial infections. The comparative analysis and 3D modeling of amino acid sequences of several omptin family proteases, such as Pla (Yersinia pestis), PgtE (Salmonella enterica), SopA (Shigella flexneri), OmpT, and OmpP (Escherichia coli), confirmed their high cross-homology with respect to the identified epitope clusters and possible involvement of individual epitopes in host–pathogen interaction.
Keywords: omptin family proteases; Gram-negative pathogens; peptide ELISA; B-cell epitope; peptide vaccine; broad-spectrum vaccine; multi-pathogen vaccine; vaccine development omptin family proteases; Gram-negative pathogens; peptide ELISA; B-cell epitope; peptide vaccine; broad-spectrum vaccine; multi-pathogen vaccine; vaccine development
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Feodorova, V.A.; Lyapina, A.M.; Zaitsev, S.S.; Khizhnyakova, M.A.; Sayapina, L.V.; Ulianova, O.V.; Ulyanov, S.S.; Motin, V.L. New Promising Targets for Synthetic Omptin-Based Peptide Vaccine against Gram-Negative Pathogens. Vaccines 2019, 7, 36.

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