Next Article in Journal
A Comparison of Plasmid DNA and mRNA as Vaccine Technologies
Previous Article in Journal
20th International Conference on Emerging Infectious Diseases in the Pacific Rim Organized by the United States-Japan Cooperative Medical Sciences Program (USJCMSP)
Open AccessArticle

New Promising Targets for Synthetic Omptin-Based Peptide Vaccine against Gram-Negative Pathogens

1
Laboratory for Molecular Biology and NanoBiotechnology, Federal Research Center for Virology and Microbiology, Branch in Saratov, 410028 Saratov, Russia
2
Department of Vaccine Control, Scientific Center on Expertise of Medical Application Products, 127051 Moscow, Russia
3
Department for Medical Optics, Saratov State University, 410012 Saratov, Russia
4
Department of Pathology, Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA
*
Authors to whom correspondence should be addressed.
Vaccines 2019, 7(2), 36; https://doi.org/10.3390/vaccines7020036
Received: 25 February 2019 / Revised: 24 March 2019 / Accepted: 4 April 2019 / Published: 10 April 2019
Omptins represent a family of proteases commonly found in various Gram-negative pathogens. These proteins play an important role in host–pathogen interaction and have been recognized as key virulence factors, highlighting the possibility of developing an omptin-based broad-spectrum vaccine. The prototypical omptin, His-tagged recombinant Pla, was used as a model target antigen. In total, 46 linear and 24 conformational epitopes for the omptin family were predicted by the use of ElliPro service. Among these we selected highly conserved, antigenic, non-allergenic, and immunogenic B-cell epitopes. Five epitopes (2, 6, 8, 10, and 11 corresponding to Pla regions 52–60, 146–150, 231–234, 286–295, and 306–311, respectively) could be the first choice for the development of the new generation of target-peptide-based vaccine against plague. The partial residues of omptin epitopes 6, 8, and 10 (regions 136–145, 227–230, and 274–285) could be promising targets for the multi-pathogen vaccine against a group of enterobacterial infections. The comparative analysis and 3D modeling of amino acid sequences of several omptin family proteases, such as Pla (Yersinia pestis), PgtE (Salmonella enterica), SopA (Shigella flexneri), OmpT, and OmpP (Escherichia coli), confirmed their high cross-homology with respect to the identified epitope clusters and possible involvement of individual epitopes in host–pathogen interaction. View Full-Text
Keywords: omptin family proteases; Gram-negative pathogens; peptide ELISA; B-cell epitope; peptide vaccine; broad-spectrum vaccine; multi-pathogen vaccine; vaccine development omptin family proteases; Gram-negative pathogens; peptide ELISA; B-cell epitope; peptide vaccine; broad-spectrum vaccine; multi-pathogen vaccine; vaccine development
Show Figures

Figure 1

MDPI and ACS Style

Feodorova, V.A.; Lyapina, A.M.; Zaitsev, S.S.; Khizhnyakova, M.A.; Sayapina, L.V.; Ulianova, O.V.; Ulyanov, S.S.; Motin, V.L. New Promising Targets for Synthetic Omptin-Based Peptide Vaccine against Gram-Negative Pathogens. Vaccines 2019, 7, 36. https://doi.org/10.3390/vaccines7020036

AMA Style

Feodorova VA, Lyapina AM, Zaitsev SS, Khizhnyakova MA, Sayapina LV, Ulianova OV, Ulyanov SS, Motin VL. New Promising Targets for Synthetic Omptin-Based Peptide Vaccine against Gram-Negative Pathogens. Vaccines. 2019; 7(2):36. https://doi.org/10.3390/vaccines7020036

Chicago/Turabian Style

Feodorova, Valentina A.; Lyapina, Anna M.; Zaitsev, Sergey S.; Khizhnyakova, Maria A.; Sayapina, Lidiya V.; Ulianova, Onega V.; Ulyanov, Sergey S.; Motin, Vladimir L. 2019. "New Promising Targets for Synthetic Omptin-Based Peptide Vaccine against Gram-Negative Pathogens" Vaccines 7, no. 2: 36. https://doi.org/10.3390/vaccines7020036

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop