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Volume 11
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10.3390/vaccines11020210
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Article

Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate

by
Patricia García
1,
Maria P. Cabral
1,2,
Alejandro Beceiro
1,3,
Miriam Moscoso
1,3 and
Germán Bou
1,3,*
1
Department of Microbiology, University Hospital A Coruña (CHUAC), Biomedical Research Institute A Coruña (INIBIC), 15006 A Coruña, Spain
2
Technical Services Department, CZ Vaccines S.A.U., 36400 O Porriño, Spain
3
Centro de Investigación Biomédica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain
*
Author to whom correspondence should be addressed.
Vaccines 2023, 11(2), 210; https://doi.org/10.3390/vaccines11020210
Submission received: 29 December 2022 / Revised: 14 January 2023 / Accepted: 15 January 2023 / Published: 17 January 2023
(This article belongs to the Special Issue Live-Attenuated Bacterial Vaccines for the Prevention of Multiresistant Infections)
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Abstract

Staphylococcus aureus is regarded as a threatening bacterial pathogen causing invasive pneumonia in healthcare settings and in the community. The continuous emergence of multidrug resistant strains is narrowing the treatment options for these infections. The development of an effective S. aureus vaccine is, therefore, a global priority. We have previously developed a vaccine candidate, 132 ΔmurI Δdat, which is auxotrophic for D-glutamate, and protects against sepsis caused by S. aureus. In the present study, we explored the potential of this vaccine candidate to prevent staphylococcal pneumonia, by using an acute lung infection model in BALB/c mice. Intranasal inoculation of the vaccine strain yielded transitory colonization of the lung tissue, stimulated production of relevant serum IgG and secretory IgA antibodies in the lung and distal vaginal mucosa and conferred cross-protection to acute pneumonia caused by clinically important S. aureus strains. Although these findings are promising, additional research is needed to minimize dose-dependent toxicity for safer intranasal immunization with this vaccine candidate.
Keywords: live-attenuated vaccine; Staphylococcus aureus; auxotrophic; intranasal immunization; glutamate racemase gene; D-amino acid transaminase gene; acute lung infection model live-attenuated vaccine; Staphylococcus aureus; auxotrophic; intranasal immunization; glutamate racemase gene; D-amino acid transaminase gene; acute lung infection model

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MDPI and ACS Style

García, P.; Cabral, M.P.; Beceiro, A.; Moscoso, M.; Bou, G. Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate. Vaccines 2023, 11, 210. https://doi.org/10.3390/vaccines11020210

AMA Style

García P, Cabral MP, Beceiro A, Moscoso M, Bou G. Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate. Vaccines. 2023; 11(2):210. https://doi.org/10.3390/vaccines11020210

Chicago/Turabian Style

García, Patricia, Maria P. Cabral, Alejandro Beceiro, Miriam Moscoso, and Germán Bou. 2023. "Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate" Vaccines 11, no. 2: 210. https://doi.org/10.3390/vaccines11020210

APA Style

García, P., Cabral, M. P., Beceiro, A., Moscoso, M., & Bou, G. (2023). Cross-Protection against Acute Staphylococcus aureus Lung Infection in Mice by a D-Glutamate Auxotrophic Vaccine Candidate. Vaccines, 11(2), 210. https://doi.org/10.3390/vaccines11020210

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