Characterization of the Efficacy of a Split Swine Influenza A Virus Nasal Vaccine Formulated with a Nanoparticle/STING Agonist Combination Adjuvant in Conventional Pigs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Vaccines and Challenge Viruses
2.2. Characterization of NanoS100–SwIAV or Nano11–SwIAV
2.3. Animal Studies
2.4. Surface and Intracellular Cytokine Labeling for Flow Cytometry
2.5. Enzyme-Linked Immunosorbent Assay (ELISA)
2.6. Virus Titration
2.7. Statistical Analysis
3. Results
3.1. Characterization of the Vaccine Formulations
3.2. NanoS100–SwIAV Vaccine Partially Decreased the Challenge Virus Titers in the Respiratory Tract of Conventional Pigs
3.3. Both Nano11–SwIAV and NanoS100–SwIAV Vaccines Downregulated the Frequencies of Activated Dendritic Cells (CD3−CD172a+SynCAM+CD80/86+) and Enhanced the Activated Monocytes (CD3−CD172a+SynCAM−CXCL10+CD80/86+) in Both Mucosal and Systemic Compartments of Vaccinates at DPC6
3.4. Both the Candidate Nano11-Based Vaccines Elicited Strong Virus-Specific IL-17A and IFNγ Secreting Recall CTL Responses in The Lung Draining TBLN of Vaccinates
3.5. Both of the Candidate Nano11 Vaccines Elicited the Challenge Virus-Specific IFNγ Secreting T Cell Responses in PBMCs of Vaccinated Pigs
3.6. Both of the Candidate Vaccines in SwIAV-H1N1-OH7-Challenged Pigs Elicited Cross-Reactive Virus-Specific Mucosal Antibody Response in Vaccinates at DPC6
3.7. NanoS100–SwIAV Vaccine Elicited Both Cross-Reactive H1N1 Pandemic Virus-Specific Lung and Systemic IgG and Lung sIgA Responses in Vaccinates at DPC6
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Phenotypes of Ivmohoid and Maeloid Cells T-Helper/Memory Cells [CD3+CD4+CD8α+CD8β−] CTLs [CD3+CD4−CD8α+CD8β+] Monocytes [CD3−CD17a+SynCAM−] | Vaccine Type | Challenge: SwlAVH1N1-OH7 | Challenge: H1N1 Pandemic |
---|---|---|---|
TBLN MNCs: CXCL10+CD80/86+ activated monocytes | NanoS100-SwlAV | *** p < 0.001 | ** p < 0.01 |
Nano11-SwlAV | *** p < 0.001 | NS | |
TBLN MNCs: CD49d+ antigen responsive T-helper/memory cells | NanoS100-SwlAV | - | - |
Nano11-SwlAV | - | * p < 0.05 | |
TBLN MNCs: IFNγ+ antigen activated CTLs | NanoS100-SwlAV | *** p < 0.05 | - |
Nano11-SwlAV | ** p < 0.01 | - | |
TBLN MNCs: CD49d+IFNγ+ antigen responsive CTLs | NanoS100-SwlAV | ** p < 0.01 | - |
Nano11-SwlAV | * p < 0.05 | - | |
TBLN MNCs: IL-17A+ antigen activated CTLs | NanoS100-SwlAV | NS | ** p < 0.01 |
Nano11-SwlAV | * p < 0.05 | * p < 0.05 | |
TBLN MNCs: CD49d+IL-17A+ antigen responsive CTLs | NanoS100-SwlAV | - | ** p < 0.01 |
Nano11-SwlAV | * p < 0.05 | ** p < 0.01 | |
PBMCs: IFNγ+ antigen activated CTLs | NanoS 100-SwlAV | - | ** p < 0.01 |
Nano11-SwlAV | - | * p < 0.05 | |
PBMCs: CD49d+IFNY+ antigen responsive CTLs | NanoS100-SwlAV | - | ** p < 0.01 |
Nano11-SwlAV | - | - |
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Patil, V.; Hernandez-Franco, J.F.; Yadagiri, G.; Bugybayeva, D.; Dolatyabi, S.; Feliciano-Ruiz, N.; Schrock, J.; Suresh, R.; Hanson, J.; Yassine, H.; et al. Characterization of the Efficacy of a Split Swine Influenza A Virus Nasal Vaccine Formulated with a Nanoparticle/STING Agonist Combination Adjuvant in Conventional Pigs. Vaccines 2023, 11, 1707. https://doi.org/10.3390/vaccines11111707
Patil V, Hernandez-Franco JF, Yadagiri G, Bugybayeva D, Dolatyabi S, Feliciano-Ruiz N, Schrock J, Suresh R, Hanson J, Yassine H, et al. Characterization of the Efficacy of a Split Swine Influenza A Virus Nasal Vaccine Formulated with a Nanoparticle/STING Agonist Combination Adjuvant in Conventional Pigs. Vaccines. 2023; 11(11):1707. https://doi.org/10.3390/vaccines11111707
Chicago/Turabian StylePatil, Veerupaxagouda, Juan F. Hernandez-Franco, Ganesh Yadagiri, Dina Bugybayeva, Sara Dolatyabi, Ninoshkaly Feliciano-Ruiz, Jennifer Schrock, Raksha Suresh, Juliette Hanson, Hadi Yassine, and et al. 2023. "Characterization of the Efficacy of a Split Swine Influenza A Virus Nasal Vaccine Formulated with a Nanoparticle/STING Agonist Combination Adjuvant in Conventional Pigs" Vaccines 11, no. 11: 1707. https://doi.org/10.3390/vaccines11111707