Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Bacterial Strains and Culture
2.3. Vaccination and Challenge Inoculation
2.4. Serological Assay
2.5. Preparation of Splenocytes
2.6. ELISpot Assay
2.7. Bacterial Strain Recovery
2.8. Statistical Analysis
3. Results
3.1. Humoral Immune Responses Induced by Vaccination
3.2. Cellular Immune Responses Induced by Vaccination
3.3. Re-Isolation of Salmonella Strains
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Skovgaard, N. Risk assessments of Salmonella in eggs and broiler chickens. Interpretative summary. Int. J. Food Microbiol. 2004, 91, 223. [Google Scholar] [CrossRef]
- Hossain, M.J.; Attia, Y.; Ballah, F.M.; Islam, M.S.; Sobur, M.A.; Islam, M.A.; Ievy, S.; Rahman, A.; Nishiyama, A.; Islam, M.S.; et al. Zoonotic Significance and Antimicrobial Resistance in Salmonella in Poultry in Bangladesh for the Period of 2011. Zoonotic Dis. 2021, 1, 3–24. [Google Scholar] [CrossRef]
- Centers for Disease Control and Prevention (CDC). National Enteric Disease Surveillance: Salmonella Annual Report. 2016. Available online: https://www.cdc.gov/nationalsurveillance/pdfs/2016-salmonella-report-508.pdf (accessed on 15 April 2018).
- Nair, D.V.T.; Johny, A.K. Food Safety in poultry meat production. In Food Safety in Poultry Meat Production; Springer: Berlin/Heidelberg, Germany, 2019; pp. 1–24. [Google Scholar] [CrossRef]
- Hafez, H.M.; Shehata, A.A. Turkey production and health: Current challenges. Ger. J. Vet. Res. 2021, 1, 3–14. [Google Scholar] [CrossRef]
- Van Immerseel, F.; De Zutter, L.; Houf, K.; Pasmans, F.; Haesebrouck, F.; Ducatelle, R. Strategies to control Salmonella in the broiler production chain. World’s Poult. Sci. J. 2009, 65, 367–392. [Google Scholar] [CrossRef]
- Babu, U.; Dalloul, R.A.; Okamura, M.; Lillehoj, H.S.; Xie, H.; Raybourne, R.B.; Gaines, D.; Heckert, R.A. Salmonella enteritidis clearance and immune responses in chickens following Salmonella vaccination and challenge. Vet. Immunol. Immunopathol. 2004, 101, 251–257. [Google Scholar] [CrossRef]
- Tellez-Isaias, G.; Vuong, C.N.; Graham, B.D.; Selby, C.M.; Graham, L.E.; Señas-Cuesta, R.; Barros, T.L.; Beer, L.C.; Coles, M.E.; Forga, A.J.; et al. Developing probiotics, prebiotics, and organic acids to control Salmonella spp. in commercial turkeys at the University of Arkansas, USA. Ger. J. Vet. Res. 2021, 1, 7–12. [Google Scholar] [CrossRef]
- Attia, Y.A.; Ellakany, H.F.; El-Hamid, A.E.A.; Bovera, F.; Ghazaly, S.A. Control of Salmonella enteritidis infection in male layer chickens by acetic acid and/or prebiotics, probiotics and antibiotics. Arch. Geflugelkd. 2012, 76, 239–245. [Google Scholar]
- Desin, T.S.; Köster, W.; Potter, A.A. Salmonella vaccines in poultry: Past, present and future. Expert Rev. Vaccines 2013, 12, 87–96. [Google Scholar] [CrossRef]
- Lillehoj, E.P.; Yun, C.H.; Lillehoj, H.S. Vaccines against the avian enteropathogens Eimeria, Cryptosporidium and Salmonella. Anim. Health Res. Rev. 2000, 1, 47–65. [Google Scholar] [CrossRef]
- Zhang-Barber, Z.; Turner, A.K.; Barrow, P.A. Vaccination for control of Salmonella in poultry. Vaccine 1999, 17, 2538–2545. [Google Scholar] [CrossRef]
- Tan, T.; Gyles, C.L.; Wilkie, B.N. Evaluation of an aroA mutant Salmonella typhimurium vaccine in chickens using modified semisolid Rappaport Vassiliadis medium to monitor faecal shedding. Vet. Microbiol. 1997, 54, 247–254. [Google Scholar] [CrossRef]
- Young, M.K.; Cox, M.M.; Calhoun, L.N. Salmonella-based vaccines for infectious diseases. Expert Rev. Vaccines 2007, 6, 147–152. [Google Scholar] [CrossRef]
- Ariaans, M.P.; van de Haar, P.M.; Lowenthal, J.W.; van Eden, W.; Hensen, E.J.; Vervelde, L. ELISPOT and intracellular cytokine staining: Novel assays for quantifying T cell responses in the chicken. Dev. Comp. Immunol. 2008, 32, 1398–1404. [Google Scholar] [CrossRef] [PubMed]
- Jia, S.; McWhorter, A.R.; Andrews, D.M.; Underwood, G.J.; Chousalkar, K.K. Challenges in vaccinating layer hens against Salmonella Typhimurium. Vaccines 2020, 8, 696. [Google Scholar] [CrossRef] [PubMed]
- Beal, R.K.; Wigley, P.; Powers, C.; Barrow, P.A.; Smith, A.L. Cross-reactive cellular and humoral immune responses to Salmonella enterica serovars Typhimurium and Enteritidis are associated with protection to heterologous re-challenge. Vet. Immunol. Immunopathol. 2006, 114, 84–93. [Google Scholar] [CrossRef]
- Mastroeni, P.; Villarreal-Ramos, B.; Hormaeche, C.E. Adoptive transfer of immunity to oral challenge with virulent salmonellae in innately susceptible BALB/c mice requires both immune serum and T cells. Infect. Immun. 1993, 61, 3981–3984. [Google Scholar] [CrossRef]
- Barrow, P.A. Salmonella infections: Immune and non-immune protection with vaccines. Avian Pathol. 2007, 36, 1–13. [Google Scholar] [CrossRef]
- Jawale, C.V.; Chaudhari, A.A.; Jeon, B.W.; Nandre, R.M.; Lee, J.H. Characterization of a novel inactivated Salmonella enterica serovar Enteritidis vaccine candidate generated using a modified cI857/λ P R/gene E expression system. Infect. Immun. 2012, 80, 1502–1509. [Google Scholar] [CrossRef]
- Desloges, N.; Schröder, I.; Schwefer, S. Development of a third generation vaccine to prevent Salmonella infections in commercial poultry flocks. Lohmann Inf. 2010, 45, 22–26. [Google Scholar]
- Nandre, R.; Matsuda, K.; Lee, J.H. Efficacy for a New Live Attenuated Salmonella Enteritidis Vaccine Candidate to Reduce Internal Egg Contamination. Zoonoses Public Health 2014, 61, 55–63. [Google Scholar] [CrossRef]
- Babu, U.; Scott, M.; Myers, M.J.; Okamura, M.; Gaines, D.; Yancy, H.F.; Lillehoj, H.; Heckert, R.A.; Raybourne, R.B. Effects of live attenuated and killed Salmonella vaccine on T-lymphocyte mediated immunity in laying hens. Vet. Immunol. Immunopathol. 2003, 91, 39–44. [Google Scholar] [CrossRef]
- Silva, E.N.; Snoeyenbos, G.H.; Weinack, O.M.; Smyser, C.F. Studies on the use of 9R strain of Salmonella gallinarum as a vaccine in chickens. Avian Dis. 1981, 25, 38–52. [Google Scholar] [CrossRef] [PubMed]
- Won, G.; Chaudhari, A.A.; Lee, J.H. Protective efficacy and immune responses by homologous prime-booster immunizations of a novel inactivated Salmonella Gallinarum vaccine candidate. Clin. Exp. Vaccine Res. 2016, 5, 148. [Google Scholar] [CrossRef] [PubMed]
- Matsuda, K.; Chaudhari, A.A.; Lee, J.H. Comparison of the safety and efficacy of a new live Salmonella Gallinarum vaccine candidate, JOL916, with the SG9R vaccine in chickens. Avian Dis. 2011, 55, 407–412. [Google Scholar] [CrossRef] [PubMed]
- Purchase, C.; Picard, J.; McDonald, R.; Bisschop, S. A comparison of the oral application and injection routes using the Onderstepoort Biological Products Fowl Typhoid vaccine, its safety, efficacy and duration of protection in commercial laying hens. J. S. Afr. Vet. Assoc. 2008, 79, 39–43. [Google Scholar] [CrossRef]
- Ismail, N.; Olano, J.P.; Feng, H.M.; Walker, D.H. Current status of immune mechanisms of killing of intracellular microorganims. FEMS Microbiol. Lett. 2002, 207, 111–120. [Google Scholar] [CrossRef]
- Bhagavan, N.V. Immunology. In Essentials of Medical Biochemistry with Clinical Cases; Academic Press: Cambridge, MA, USA, 2011; pp. 449–472. ISBN 0120954613. [Google Scholar]
- Xu, H.; Timares, L.; Elmets, C.A. Host Defenses in Skin, 5th ed.; Elsevier Ltd.: Amsterdam, The Netherlands, 2019; Volume 2. [Google Scholar]
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Shin, H.; La, T.-M.; Lee, H.-J.; Kim, T.; Song, S.-u.; Park, E.; Park, G.-H.; Choi, I.-S.; Park, S.-Y.; Lee, J.-B.; et al. Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens. Vaccines 2022, 10, 1405. https://doi.org/10.3390/vaccines10091405
Shin H, La T-M, Lee H-J, Kim T, Song S-u, Park E, Park G-H, Choi I-S, Park S-Y, Lee J-B, et al. Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens. Vaccines. 2022; 10(9):1405. https://doi.org/10.3390/vaccines10091405
Chicago/Turabian StyleShin, Hyunjin, Tae-Min La, Hong-Jae Lee, Taesoo Kim, Seung-un Song, Eunjin Park, Gyu-Hyung Park, In-Soo Choi, Seung-Yong Park, Joong-Bok Lee, and et al. 2022. "Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens" Vaccines 10, no. 9: 1405. https://doi.org/10.3390/vaccines10091405
APA StyleShin, H., La, T.-M., Lee, H.-J., Kim, T., Song, S.-u., Park, E., Park, G.-H., Choi, I.-S., Park, S.-Y., Lee, J.-B., & Lee, S.-W. (2022). Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens. Vaccines, 10(9), 1405. https://doi.org/10.3390/vaccines10091405