A Cloned Recombinant Vesicular Stomatitis Virus-Vectored Marburg Vaccine, PHV01, Protects Guinea Pigs from Lethal Marburg Virus Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Ethics and Biosafety Statement
2.2. Viruses and Cells
2.3. VSV-Based Vaccines
2.4. Guinea Pig Study Design
2.5. VSV and MARV RNA Quantification
2.6. IgG ELISAs
2.7. Plaque Reduction Neutralization Test (PRNT)
2.8. Statistical Analyses
3. Results
3.1. Vaccination with PHV01 Protects Guinea Pigs from Lethal GPA-MARV/Ang Infection
3.2. Vaccinated Animals Exhibited Transient Vaccinemia
3.3. The Majority of Vaccinated Animals Exhibit No MARV Viremia
3.4. Vaccinated Animals Mount a Robust Humoral Response to MARV GP
3.5. Vaccinated Animals Exhibit a Neutralizing Antibody Response
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhu, W.; Liu, G.; Cao, W.; He, S.; Leung, A.; Ströher, U.; Fairchild, M.J.; Nichols, R.; Crowell, J.; Fusco, J.; et al. A Cloned Recombinant Vesicular Stomatitis Virus-Vectored Marburg Vaccine, PHV01, Protects Guinea Pigs from Lethal Marburg Virus Disease. Vaccines 2022, 10, 1004. https://doi.org/10.3390/vaccines10071004
Zhu W, Liu G, Cao W, He S, Leung A, Ströher U, Fairchild MJ, Nichols R, Crowell J, Fusco J, et al. A Cloned Recombinant Vesicular Stomatitis Virus-Vectored Marburg Vaccine, PHV01, Protects Guinea Pigs from Lethal Marburg Virus Disease. Vaccines. 2022; 10(7):1004. https://doi.org/10.3390/vaccines10071004
Chicago/Turabian StyleZhu, Wenjun, Guodong Liu, Wenguang Cao, Shihua He, Anders Leung, Ute Ströher, Michael J. Fairchild, Rick Nichols, Joseph Crowell, Joan Fusco, and et al. 2022. "A Cloned Recombinant Vesicular Stomatitis Virus-Vectored Marburg Vaccine, PHV01, Protects Guinea Pigs from Lethal Marburg Virus Disease" Vaccines 10, no. 7: 1004. https://doi.org/10.3390/vaccines10071004
APA StyleZhu, W., Liu, G., Cao, W., He, S., Leung, A., Ströher, U., Fairchild, M. J., Nichols, R., Crowell, J., Fusco, J., & Banadyga, L. (2022). A Cloned Recombinant Vesicular Stomatitis Virus-Vectored Marburg Vaccine, PHV01, Protects Guinea Pigs from Lethal Marburg Virus Disease. Vaccines, 10(7), 1004. https://doi.org/10.3390/vaccines10071004