Placental Adaptive Changes to Protect Function and Decrease Oxidative Damage in Metabolically Healthy Maternal Obesity
Abstract
:1. Introduction
2. Material and Methods
2.1. Products and Antibodies
2.2. Study Subjects
2.3. Tissue Collection and Placental Samples
2.4. Protein Extract Preparation
2.5. Measurement of Total Protein Expression by SDS-Western Blotting Assay
2.6. Determination of Oxidative Status Markers
2.6.1. Lipid Peroxidation (LPO)
2.6.2. Carbonyl Groups (C=O)
2.7. Determination of Antioxidant Defense Markers
2.7.1. Native SDS-PAGE SOD Activity
2.7.2. Catalase Activity
2.7.3. Total Antioxidant Capacity
2.8. Nitrosative Stress Markers
2.8.1. S-Nitrosylated Protein Detection by Biotin Switch Assay
2.8.2. Detection and Quantification of Nitrated Proteins and iNOS by SDS-PAGE Blotting
2.9. Data Analysis
3. Results
3.1. Clinical and Anthropometric Characteristics
3.2. Assesesment of Placental Antioxidant Defense Markers
3.3. Assesesment of Placental Oxidative Damage Markers
3.4. Nitrosative Stress Markers
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Characteristics | Lean Group (n = 9) | Obese Group (n = 8) |
---|---|---|
Delivery mode | Caesarean section (No labor) | Caesarean section (No labor) |
Maternal age (years) | 33.1 ± 6.3 | 35.5 ± 6.4 |
Gestational age at delivery (weeks) | 38.3 ± 1.0 | 38.3 ± 0.8 |
Pregravid Maternal BMI (kg/m2) | 22.5 ± 1.3 | 35.5 ± 6.4 * |
Maternal BMI at delivery (kg/m2) | 28.1 ± 2.5 | 37.3 ± 5.7 * |
Birthweight (g) | 3182 ± 214 | 3170 ± 336 |
F/M sex ratio | 5/4 | 5/3 |
Placental weight (g) | 603 ± 75 | 604 ± 54 |
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Santos-Rosendo, C.; Bugatto, F.; González-Domínguez, A.; Lechuga-Sancho, A.M.; Mateos, R.M.; Visiedo, F. Placental Adaptive Changes to Protect Function and Decrease Oxidative Damage in Metabolically Healthy Maternal Obesity. Antioxidants 2020, 9, 794. https://doi.org/10.3390/antiox9090794
Santos-Rosendo C, Bugatto F, González-Domínguez A, Lechuga-Sancho AM, Mateos RM, Visiedo F. Placental Adaptive Changes to Protect Function and Decrease Oxidative Damage in Metabolically Healthy Maternal Obesity. Antioxidants. 2020; 9(9):794. https://doi.org/10.3390/antiox9090794
Chicago/Turabian StyleSantos-Rosendo, Celeste, Fernando Bugatto, Alvaro González-Domínguez, Alfonso M. Lechuga-Sancho, Rosa Maria Mateos, and Francisco Visiedo. 2020. "Placental Adaptive Changes to Protect Function and Decrease Oxidative Damage in Metabolically Healthy Maternal Obesity" Antioxidants 9, no. 9: 794. https://doi.org/10.3390/antiox9090794