Peroxiredoxin II Maintains the Mitochondrial Membrane Potential against Alcohol-Induced Apoptosis in HT22 Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Cell Culture
2.3. Transduction of HT22 Cells with Lentiviral-Delivered Prx II Wild Type and Prx II siRNA
2.4. Cell Viability Assay
2.5. Detection of ROS and Superoxide Anion
2.6. Flow Cytometric Detection of Mitochondrial Membrane Potential
2.7. Detection of Apoptosis
2.8. Western Blot
2.9. Electron Microscopy Analysis
2.10. Statistical Analysis
3. Results
3.1. Prx II Knockdown Elevated Alcohol Induced Apoptosis and ROS Accumulation in HT22 Cells
3.2. Knockdown of Prx II Elevated the Alcohol-Induced Mitochondria ROS and Membrane Permeability in HT22 Cells
3.3. Knockdown of Prx II Results in Up-Regulating the Mitochondria Dependent Cellular Apoptosis
3.4. Regulatory Function of Prx II on Alcohol-Induced Mitochondria Dysfunctions is Dependent on Cellular ROS Levels
3.5. NAC Treatment Restores the Alcohol-Induced Up-Regulating of the Apoptosis-Related Protein Expressions in shPrx II HT22 Cells
3.6. Prx II Inhibits the Cellular Apoptosis by Down-Regulating the Mitochondria-Dependent Apoptotic Pathway
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Jin, M.-H.; Yu, J.-B.; Sun, H.-N.; Jin, Y.-H.; Shen, G.-N.; Jin, C.-H.; Cui, Y.-D.; Lee, D.-S.; Kim, S.-U.; Kim, J.-S.; et al. Peroxiredoxin II Maintains the Mitochondrial Membrane Potential against Alcohol-Induced Apoptosis in HT22 Cells. Antioxidants 2020, 9, 1. https://doi.org/10.3390/antiox9010001
Jin M-H, Yu J-B, Sun H-N, Jin Y-H, Shen G-N, Jin C-H, Cui Y-D, Lee D-S, Kim S-U, Kim J-S, et al. Peroxiredoxin II Maintains the Mitochondrial Membrane Potential against Alcohol-Induced Apoptosis in HT22 Cells. Antioxidants. 2020; 9(1):1. https://doi.org/10.3390/antiox9010001
Chicago/Turabian StyleJin, Mei-Hua, Jia-Bin Yu, Hu-Nan Sun, Ying-Hua Jin, Gui-Nan Shen, Cheng-Hao Jin, Yu-Dong Cui, Dong-Seok Lee, Sun-Uk Kim, Ji-Su Kim, and et al. 2020. "Peroxiredoxin II Maintains the Mitochondrial Membrane Potential against Alcohol-Induced Apoptosis in HT22 Cells" Antioxidants 9, no. 1: 1. https://doi.org/10.3390/antiox9010001