Next Article in Journal
Anti-Hyperglycemic and Anticholinergic Effects of Natural Antioxidant Contents in Edible Flowers
Previous Article in Journal
Caffeoylquinic Acids and Flavonoids of Fringed Sagewort (Artemisia frigida Willd.): HPLC-DAD-ESI-QQQ-MS Profile, HPLC-DAD Quantification, in Vitro Digestion Stability, and Antioxidant Capacity
Article Menu

Export Article

Open AccessArticle

In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats

1
Department of Human Biology, Faculty of Health Sciences, Walter Sisulu University, Mthatha 5117, South Africa
2
Department of Chemistry, Faculty of Sciences, Walter Sisulu University, Mthatha 5117, South Africa
3
Department of Chemistry, Faculty of Sciences, University of Fort Hare, Alice 5700, South Africa
4
Department of Biological and Environmental Sciences, Faculty of Sciences, Walter Sisulu University, Mthatha 5117, South Africa
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(8), 309; https://doi.org/10.3390/antiox8080309
Received: 27 June 2019 / Revised: 24 July 2019 / Accepted: 2 August 2019 / Published: 15 August 2019
(This article belongs to the Section Health Outcomes of Antioxidants and Oxidative Stress)
  |  
PDF [297 KB, uploaded 15 August 2019]

Abstract

Oxidative stress has gained attention as one of the fundamental mechanisms responsible for the development of hypertension. The present study investigated in vitro and in vivo antioxidant effects of 70% ethanol-water (v/v) leaf and root extracts of T. officinale (TOL and TOR, respectively). Total phenolic and flavonoid content of plant extracts were assessed using Folin Ciocalteau and aluminium chloride colorimetric methods; while, 2,2-diphenyl-1-picrlhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) protocols were used to determine the free radical scavenging and total antioxidant capacities (TAC), respectively. The in vivo total antioxidant capacity and malondialdehyde acid (MDA) levels for lipid peroxidation tests were performed on organ homogenate samples from Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive rats treated with leaf extract, TOL (500 mg/kg/day) and TOR (500 mg/kg/day) for 21 days. Results showed that compared to TOR, TOL possessed significantly higher (p < 0.01) polyphenol (4.35 ± 0.15 compared to 1.14 ± 0.01) and flavonoid (23.17 ± 0.14 compared to 3 ± 0.05) content; free radical scavenging activity (EC50 0.37 compared to 1.34 mg/mL) and total antioxidant capacities (82.56% compared to 61.54% ABTS, and 156 ± 5.28 compared to 40 ± 0.31 FRAP) and both extracts showed no toxicity (LD50 > 5000 mg/kg). TOL and TOR significantly (p < 0.01) elevated TAC and reduced MDA levels in targets organs. In conclusion, T. officinale leaf extract possesses significant anti-oxidant effects which conferred significant in vivo antioxidant protection against free radical-mediated oxidative stress in L-NAME-induced hypertensive rats. View Full-Text
Keywords: oxidative stress; acute toxicity; antioxidants; L-NAME; DPPH; FRAP; ABTS oxidative stress; acute toxicity; antioxidants; L-NAME; DPPH; FRAP; ABTS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Aremu, O.O.; Oyedeji, A.O.; Oyedeji, O.O.; Nkeh-Chungag, B.N.; Rusike, C.R.S. In Vitro and In Vivo Antioxidant Properties of Taraxacum officinale in Nω-Nitro-l-Arginine Methyl Ester (L-NAME)-Induced Hypertensive Rats. Antioxidants 2019, 8, 309.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Antioxidants EISSN 2076-3921 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top