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Erigeron annuus (L.) Pers. Extract Inhibits Reactive Oxygen Species (ROS) Production and Fat Accumulation in 3T3-L1 Cells by Activating an AMP-Dependent Kinase Signaling Pathway

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Department of Food and Nutrition, Hallym Polytechnic University, Chuncheon 24210, Korea
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Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, Korea
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Department of Food Science and Nutrition, Hallym University, Chuncheon 24252, Korea
*
Author to whom correspondence should be addressed.
Antioxidants 2019, 8(5), 139; https://doi.org/10.3390/antiox8050139
Received: 18 April 2019 / Revised: 8 May 2019 / Accepted: 20 May 2019 / Published: 20 May 2019
Obesity is one of the major public health problems in the world because it is implicated in metabolic syndromes, such as type 2 diabetes, hypertension, and cardiovascular diseases. The objective of this study was to investigate whether Erigeron annuus (L.) Pers. (EAP) extract suppresses reactive oxygen species (ROS) production and fat accumulation in 3T3-L1 cells by activating an AMP-dependent kinase (AMPK) signaling pathway. Our results showed that EAP water extract significantly inhibits ROS production, adipogenesis, and lipogenesis during differentiation of 3T3-L1 preadipocytes. In addition, EAP decreased mRNA and protein levels of proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBPα). Moreover, EAP suppressed mRNA expressions of fatty acid synthase (FAS), lipoprotein lipase (LPL), adipocyte protein 2 (aP2) in a dose-dependent manner. Whereas, EAP upregulated adiponectin expression, phosphorylation levels of AMPK and carnitine palmitoyltransferase 1 (CPT-1) protein level during differentiation of 3T3-L1 preadipocytes. These results suggest that EAP water extract can exert ROS-linked anti-obesity effect through the mechanism that might involve inhibition of ROS production, adipogenesis and lipogenesis via an activating AMPK signaling pathway. View Full-Text
Keywords: Erigeron annuus (L.) Pers; 3T3-L1 adipocyte; ROS production; fat accumulation; AMPK signaling pathway Erigeron annuus (L.) Pers; 3T3-L1 adipocyte; ROS production; fat accumulation; AMPK signaling pathway
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Choi, Y.-H.; Lee, O.-H.; Zheng, Y.; Kang, I.-J. Erigeron annuus (L.) Pers. Extract Inhibits Reactive Oxygen Species (ROS) Production and Fat Accumulation in 3T3-L1 Cells by Activating an AMP-Dependent Kinase Signaling Pathway. Antioxidants 2019, 8, 139.

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