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Antioxidants 2018, 7(4), 57; https://doi.org/10.3390/antiox7040057

Selenium-Rich Ricegrass Juice Improves Antioxidant Properties and Nitric Oxide Inhibition in Macrophage Cells

1
Interdisciplinary Graduate School of Nutraceutical and Functional Food, Prince of Songkla University, Hat-Yai, Songkla 90112, Thailand
2
Department of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkla 90112, Thailand
3
Department of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Graz 8010, Austria
4
Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat-Yai, Songkla 90112, Thailand
*
Author to whom correspondence should be addressed.
Received: 20 February 2018 / Revised: 29 March 2018 / Accepted: 5 April 2018 / Published: 13 April 2018
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Abstract

Ricegrass juice (Oryza sativa L.) was introduced as a functional food as the consumption of sprouts or seedlings has been claimed to provide high nutritive value. Selenium (Se) is a trace mineral that plays a key role in the human antioxidation scheme. Supplementation of Se into plants is one strategy to enhance plant bioactivities, and the consumption of Se plant foods may confer superior health benefits. In this study, ricegrass juice extract was analyzed for its major phenolic components. The effect of ricegrass juice extracts bio-fortified with 0, 10 and 40 mg Se/L named as RG0, RG10, and RG40, respectively, were investigated for a percentage of cell viability, changes of endogenous antioxidant enzymes, lipid peroxidation, and nitric oxide inhibition in RAW264.7 macrophage cells. Flavone glycosides, namely chrysoeriol arabinosyl arabinoside derivatives, were found to be the foremost bioactive components in ricegrass juice extract indicated by UHPLC-MS. The results of cell culture assessment revealed that RG40 showed an ability to promote macrophage cell proliferation at low concentration. Ricegrass juice extract in all treatments possessed the ability to reduce malondialdehyde content, which may be regarded as the bioactivity of phenolic compounds. Moreover, Se also played a role in this effect since RG40 showed the greatest ability via increasing the level of GPx enzyme. It was also discovered that phenolic compounds in the extracts played a role in inhibiting nitric oxide in LPS-induced RAW264.7 cells. Furthermore, RG40 expressed significantly higher NO inhibition properties at IC50 118.76 µg/mL compared to RG0 and RG10, at 147.02 and 147.73 µg/mL, respectively. Se bio-fortified ricegrass juice could be considered as a new potent functional food that can lower the risk of oxidative stress and chronic inflammation diseases. View Full-Text
Keywords: selenium; ricegrass juice; antioxidant enzymes; lipid peroxidation; nitric oxide inhibition selenium; ricegrass juice; antioxidant enzymes; lipid peroxidation; nitric oxide inhibition
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Chomchan, R.; Puttarak, P.; Brantner, A.; Siripongvutikorn, S. Selenium-Rich Ricegrass Juice Improves Antioxidant Properties and Nitric Oxide Inhibition in Macrophage Cells. Antioxidants 2018, 7, 57.

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