Comparing the Role of ROS and RNS in the Thermal Stress Response of Two Cnidarian Models, Exaiptasia diaphana and Galaxea fascicularis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Identification of Symbiodiniacean Communities from Galaxea fascicularis Colonies
2.2. Experimental Setups and Sample Processing
2.3. Photosynthetic Performance and Respiration
2.4. Symbiodiniacean Cell Density, Sea Anemone Host Protein, and Coral Surface Area
2.5. ROS and RNS Quantification in Freshly Isolated Symbionidiaceae by Flow Cytometry
2.6. Enzyme Activities Involved in ROS Scavenging and RNS Synthesis
2.7. Data Analysis
3. Results
3.1. Bleaching Metrics of Exaiptasia diaphana and Galaxea fascicularis
3.2. Net ROS and RNS Production during Thermal Stress in Exaiptasia diaphana and Galaxea fascicularis
3.3. Activities of ROS-Scavenging and RNS-Synthesizing Enzymes during Thermal Stress in Exaiptasia diaphana and Galaxea fascicularis
4. Discussion
4.1. GBR-Sourced Exaiptasia diaphana Is Not a Suitable Model to Study Coral Bleaching
4.2. The Oxidative Stress Response during Thermal Stress Was More Marked in Galaxea fascicularis than in Exaiptasia diaphana and Aligned with the Extent of Physiological Stress
4.3. Ambiguous Role of RNS in the Thermal Stress Response of Exaiptasia diaphana and Galaxea fascicularis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Doering, T.; Maire, J.; Chan, W.Y.; Perez-Gonzalez, A.; Meyers, L.; Sakamoto, R.; Buthgamuwa, I.; Blackall, L.L.; van Oppen, M.J.H. Comparing the Role of ROS and RNS in the Thermal Stress Response of Two Cnidarian Models, Exaiptasia diaphana and Galaxea fascicularis. Antioxidants 2023, 12, 1057. https://doi.org/10.3390/antiox12051057
Doering T, Maire J, Chan WY, Perez-Gonzalez A, Meyers L, Sakamoto R, Buthgamuwa I, Blackall LL, van Oppen MJH. Comparing the Role of ROS and RNS in the Thermal Stress Response of Two Cnidarian Models, Exaiptasia diaphana and Galaxea fascicularis. Antioxidants. 2023; 12(5):1057. https://doi.org/10.3390/antiox12051057
Chicago/Turabian StyleDoering, Talisa, Justin Maire, Wing Yan Chan, Alexis Perez-Gonzalez, Luka Meyers, Rumi Sakamoto, Isini Buthgamuwa, Linda L. Blackall, and Madeleine J. H. van Oppen. 2023. "Comparing the Role of ROS and RNS in the Thermal Stress Response of Two Cnidarian Models, Exaiptasia diaphana and Galaxea fascicularis" Antioxidants 12, no. 5: 1057. https://doi.org/10.3390/antiox12051057