Association Study between Antioxidant Nutrient Intake and Low Bone Mineral Density with Oxidative Stress-Single Nucleotide Variants: GPX1 (rs1050450 and rs17650792), SOD2 (rs4880) and CAT (rs769217) in Mexican Women
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population
2.2. Bone Mineral Density Measurement (Assessment)
2.3. Anthropometric and Clinical Evaluation
2.4. DNA Extraction and Genotyping Analysis
2.5. Dietary Assessment
2.6. Statistical Analysis
3. Results
3.1. Population Characteristics
3.2. Allele Frequencies of SNVs
3.3. Association Analyses between Oxidative Stress-Related SNVs and Bone Mineral Density
3.4. Dietary Antioxidant Intake and Oxidative Stress-Related SNV Association
3.5. Association Analyses between Oxidative Stress-Related SNVs and Metabolic Traits
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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rs1050450 (GPX1) | rs17650792 (GPX1) | rs4880 (SOD2) | rs769217 (CAT) | |||||||
---|---|---|---|---|---|---|---|---|---|---|
BMD Site | Model | Genotype | b (95%CI) | p Value | b (95%CI) | p Value | b (95%CI) | p Value | b (95%CI) | p Value |
Total hip | Additive | −0.003 (−0.017, 0.010) | 0.617 | −0.001 (−0.012, 0.010) | 0.806 | 0.007 (−0.003, 0.016) | 0.181 | 0.0001 (−0.010, 0.011) | 0.980 | |
Codominant | GG | 0.0 | 0.0 | 0.0 | 0.0 | |||||
GA | −0.0003 (−0.016, 0.016) | 0.975 | 0.010 (−0.004, 0.024) | 0.155 | 0.002 (−0.012, 0.016) | 0.796 | 0.011 (−0.003, 0.025) | 0.131 | ||
AA | −0.022 (−0.069, 0.025) | 0.359 | −0.027 (−0.056, 0.001) | 0.063 | 0.018 (−0.004, 0.039) | 0.110 | −0.017 (−0.043, 0.009) | 0.196 | ||
Dominant | GG | 0.0 | 0.0 | 0.0 | 0.0 | |||||
GA + AA | −0.002 (−0.017, 0.013) | 0.791 | 0.005 (−0.009, 0.018) | 0.475 | 0.005 (−0.008, 0.019) | 0.449 | 0.006 (−0.007, 0.020) | 0.341 | ||
Recessive | GG + GA | 0.0 | 0.0 | 0.0 | 0.0 | |||||
AA | −0.022 (−0.069, 0.025) | 0.359 | −0.031 (−0.060,−0.003) | 0.031 | 0.017 (−0.004, 0.037) | 0.108 | −0.022 (−0.047, 0.003) | 0.085 | ||
Femoral neck | Additive | −0.006 (−0.019, 0.007) | 0.355 | −0.004 (−0.015, 0.007) | 0.452 | 0.005 (−0.005, 0.014) | 0.336 | 0.002 (−0.008, 0.013) | 0.634 | |
Codominant | GG | 0.0 | 0.0 | 0.0 | 0.0 | |||||
GA | −0.004 (−0.019, 0.012) | 0.642 | 0.007 (−0.006, 0.021) | 0.294 | 0.004 (−0.010, 0.018) | 0.579 | 0.012 (−0.001, 0.026) | 0.080 | ||
AA | −0.025 (−0.071, 0.021) | 0.293 | −0.033 (−0.061,−0.005) | 0.010 (−0.010, 0.031) | 0.346 | −0.011 (−0.036, 0.014) | 0.408 | |||
Dominant | GG | 0.0 | 0.0 | 0.0 | 0.0 | |||||
GA + AA | −0.005 (−0.020, 0.009) | 0.477 | 0.002 (−0.011, 0.015) | 0.797 | 0.005 (−0.008, 0.018) | 0.434 | 0.009 (−0.004, 0.022) | 0.193 | ||
Recessive | GG + GA | 0.0 | 0.0 | 0.0 | 0.0 | |||||
AA | −0.024 (−0.079, 0.022) | 0.307 | −0.035 (−0.063,−0.008) | 0.012 | 0.008 (−0.012, 0.028) | 0.423 | −0.016 (−0.040, 0.008) | 0.191 |
rs1050450 | rs17650792 | rs4880 | rs769217 | |||||||
---|---|---|---|---|---|---|---|---|---|---|
BMD Site | Model | Genotype | OR (95%CI) | p Value | OR (95%CI) | p Value | OR (95%CI) | p Value | OR (95%CI) | p Value |
Total hip | Additive | 1.02 (0.74–1.41) | 0.909 | 1.16 (0.89–1.50) | 0.267 | 0.88 (0.70–1.11) | 0.277 | 1.06 (0.83–1.36) | 0.639 | |
Codominant | GG | 1.0 | 1.0 | 1.0 | 1.0 | |||||
GA | 1.00 (0.69–1.44) | 0.991 | 1.02 (0.73–1.41) | 0.924 | 1.11 (0.80–1.53) | 0.541 | 0.93 (0.67–1.29) | 0.655 | ||
AA | 1.19 (0.34–4.16) | 0.783 | 1.77 (0.91–3.45) | 0.092 | 0.61 (0.36–1.05) | 0.072 | 1.37 (0.77–2.43) | 0.286 | ||
Dominant | GG | 1.0 | 1.0 | 1.0 | 1.0 | |||||
GA + AA | 1.01 (0.71–1.44) | 0.962 | 1.09 (0.80–1.50) | 0.569 | 0.99 (0.72–1.35) | 0.928 | 0.99 (0.72–1.35) | 0.939 | ||
Recessive | GG + GA | 1.0 | 1.0 | 1.0 | 1.0 | |||||
AA | 1.19 (0.34–4.15) | 0.782 | 1.76 (0.92–3.39) | 0.090 | 0.58 (0.35–0.96) | 0.036 | 1.42 (0.81–2.47) | 0.217 | ||
Femoral neck | Additive | 1.36 (1.02–1.82) | 0.038 | 1.34 (1.05–1.70) | 0.017 | 0.89 (0.72–1.10) | 0.288 | 0.93 (0.74–1.17) | 0.552 | |
Codominant | GG | 1.0 | 1.0 | 1.0 | 1.0 | |||||
GA | 1.34 (0.96–1.88) | 0.090 | 1.10 (0.82–1.49) | 0.523 | 0.83 (0.61–1.13) | 0.232 | 0.88 (0.65–1.19) | 0.411 | ||
AA | 2.01 (0.72–5.58) | 0.182 | 2.79 (1.46–5.33) | 0.002 | 0.85 (0.53–1.34) | 0.477 | 0.95 (0.55–1.65) | 0.863 | ||
Dominant | GG | 1.0 | 1.0 | 1.0 | 1.0 | |||||
GA + AA | 1.38 (0.99–1.92) | 0.053 | 1.24 (0.93–1.66) | 0.137 | 0.83 (0.63–1.11) | 0.216 | 0.89 (0.67–1.19) | 0.438 | ||
Recessive | GG + GA | 1.0 | 1.0 | 1.0 | 1.0 | |||||
AA | 1.88 (0.68–5.20) | 0.226 | 2.69 (1.42–5.10) | 0.002 | 0.93 (0.60–1.43) | 0.741 | 1.01 (0.60–1.72) | 0.968 |
rs1050450 | rs17650792 | ||||||||
---|---|---|---|---|---|---|---|---|---|
Vitamin B12 (mg/day) | Omega 6 (g/day) | DAQS | DAQS | ||||||
Model | Genotype | Coefficient & (95%CI) | p Value | Coefficient & (95%CI) | p Value | OR (95%CI) | p Value | OR (95%CI) | p Value |
Additive | 0.16 (0.01, 0.31) | 0.035 | 0.30 (−0.09, 0.69) | 0.125 | 1.54 (1.11–2.13) | 0.009 | 1.30 (1.00, 1.71) | 0.054 | |
Codominant | GG | 0.0 | 0.0 | 1.0 | 1.0 | ||||
GA | 0.23 (0.06, 0.41) | 0.01 | 0.50 (0.05, 0.96) | 0.030 | 1.76 (1.21–2.56) | 0.003 | 1.19 (0.85–1.66) | 0.324 | |
AA | −0.32 (0.84, 0.20) | 0.225 | 0.20 (−1.12, 1.52) | 0.768 | 1.22 (0.38–3.87) | 0.736 | 2.12 (1.03–4.38) | 0.042 | |
Dominant | GG | 0.0 | 0.0 | 1.0 | 1.0 | ||||
GA + AA | 0.19 (0.02, 0.36) | 0.029 | 0.49 (0.06, 0.93) | 0.026 | 1.71 (1.19–2.46) | 0.004 | 1.28 (0.92–1.77) | 0.141 | |
Recessive | GG + GA | 0.0 | 0.0 | 1.0 | 1.0 | ||||
AA | −0.36 (−0.89, 0.17) | 0.181 | 0.11 (−1.18, 1.41) | 0.864 | 1.07 (0.34–3.35) | 0.913 | 1.98 (0.98–4.05) | 0.058 |
Selenium (µg/day) | Glucose levels (mg/dL) | Type 2 Diabetes | |||||
---|---|---|---|---|---|---|---|
Model | Genotype | Coefficient (95%CI) & | p Value | β (95%CI) | p Value | OR (95%CI) | p Value |
Additive | 0.63 (−0.86, 2.11) | 0.410 | 0.02 (−0.0006, 0.03) | 0.058 | 1.29 (1.01–1.66) | 0.043 | |
Codominant | TT | 0.0 | 0.0 | 1.0 | |||
TC | −1.24 (−3.1, 0.60) | 0.186 | 0.003 (−0.02, 0.03) | 0.813 | 1.24 (0.89–1.74) | 0.208 | |
CC | 3.43 (−0.06, 6.92) | 0.054 | 0.06 (0.02, 0.10) | 0.008 | 1.77 (0.99–3.14) | 0.052 | |
Dominant | TT | 0.0 | 0.0 | 1.0 | |||
TC + CC | −0.74 (−2.54, 1.06) | 0.416 | 0.01 (−0.01, 0.03) | 0.33 | 1.31 (0.95–1.81) | 0.098 | |
Recessive | TT + TC | 0.0 | 0.0 | 1.0 | |||
CC | 4.21 (0.76, 7.66) | 0.017 | 0.06 (0.02, 0.10) | 0.007 | 1.59 (0.92–2.76) | 0.098 |
Saturated fat (g/day) | Fiber (g/day) | Riboflavin (mg/day) | Vitamin B6 (mg/day) | Vitamin D (UI/day) | Vitamin E (mg/day) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Model | Genotype | Coefficient (95%CI) | p Value | Coefficient (95%CI) | p Value | Coefficient (95%CI) | p Value | Coefficient (95%CI) | p Value | Coefficient (95%CI) | p Value | Coefficient (95%CI) | p Value |
Additive | −0.25 (−0.69, 0.19) | 0.27 | −0.70 (−1.47, 0.07) | 0.073 | 0.04 (0.007, 0.08) | 0.021 | −0.03 (−0.06, 0.01) | 0.167 | 0.28 (0.04, 0.53) | 0.025 | −0.09 (−0.26, 0.08) | 0.282 | |
Codominant | GG | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | ||||||
GA | −0.92 (−1.56–0.30) | 0.004 | −0.14 (−1.18, 0.90) | 0.796 | 0.03 (−0.03, 0.08) | 0.310 | 0.002 (−0.05, 0.06) | 0.949 | 0.05 (−0.30, 0.40) | 0.767 | 0.009 (−0.24, 0.26) | 0.942 | |
AA | 0.17 (−0.81–1.14) | 0.740 | −2.23 (−3.83–0.62) | 0.007 | 0.12 (0.04, 0.20) | 0.005 | −0.10 (−0.18–0.007) | 0.034 | 0.85 (0.31, 1.39) | 0.002 | −0.37 (−0.75, 0.02) | 0.060 | |
Dominant | GG | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | ||||||
GA + AA | −0.69 (−1.29–0.09) | 0.024 | −0.44 (−1.47, 0.58) | 0.394 | 0.04 (0.02, 0.09) | 0.171 | −0.02 (−0.07, 0.04) | 0.508 | 0.11 (−0.21, 0.44) | 0.491 | −0.04 (−0.27, 0.19) | 0.723 | |
Recessive | GG + GA | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | ||||||
AA | 0.60 (−0.33, 1.53) | 0.207 | −2.27 (−3.75–0.79) | 0.003 | 0.10 (0.02, 0.18) | 0.012 | −0.10 (−0.18–0.01) | 0.024 | 0.79 (0.28, 1.30) | 0.002 | −0.37 (−0.74–0.01) | 0.043 |
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Jiménez-Ortega, R.F.; Aparicio-Bautista, D.I.; Becerra-Cervera, A.; López-Montoya, P.; León-Reyes, G.; Flores-Morales, J.; Castillejos-López, M.; Hidalgo-Bravo, A.; Salmerón, J.; Rivera-Paredez, B.; et al. Association Study between Antioxidant Nutrient Intake and Low Bone Mineral Density with Oxidative Stress-Single Nucleotide Variants: GPX1 (rs1050450 and rs17650792), SOD2 (rs4880) and CAT (rs769217) in Mexican Women. Antioxidants 2023, 12, 2089. https://doi.org/10.3390/antiox12122089
Jiménez-Ortega RF, Aparicio-Bautista DI, Becerra-Cervera A, López-Montoya P, León-Reyes G, Flores-Morales J, Castillejos-López M, Hidalgo-Bravo A, Salmerón J, Rivera-Paredez B, et al. Association Study between Antioxidant Nutrient Intake and Low Bone Mineral Density with Oxidative Stress-Single Nucleotide Variants: GPX1 (rs1050450 and rs17650792), SOD2 (rs4880) and CAT (rs769217) in Mexican Women. Antioxidants. 2023; 12(12):2089. https://doi.org/10.3390/antiox12122089
Chicago/Turabian StyleJiménez-Ortega, Rogelio F., Diana I. Aparicio-Bautista, Adriana Becerra-Cervera, Priscilla López-Montoya, Guadalupe León-Reyes, Jeny Flores-Morales, Manuel Castillejos-López, Alberto Hidalgo-Bravo, Jorge Salmerón, Berenice Rivera-Paredez, and et al. 2023. "Association Study between Antioxidant Nutrient Intake and Low Bone Mineral Density with Oxidative Stress-Single Nucleotide Variants: GPX1 (rs1050450 and rs17650792), SOD2 (rs4880) and CAT (rs769217) in Mexican Women" Antioxidants 12, no. 12: 2089. https://doi.org/10.3390/antiox12122089