Integration of Transcriptomics and Proteomics Analysis Reveals the Molecular Mechanism of Eriocheir sinensis Gills Exposed to Heat Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal and Acute Heat Stress Treatment
2.2. Transcriptome Sequencing and Analysis
2.3. Proteome Sequencing and Analysis
2.4. Screening of Genes and Proteins Related to Antioxidation
2.5. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
3. Results
3.1. Overall Statistics for Transcriptomic and Proteomic Sequencing
3.2. Comparison and Enrichment Analysis of DEGs
3.3. Comparison and Enrichment Analysis of DEPs
3.4. Integrative Analysis of the Transcriptome and Proteome
4. Discussion
4.1. The Differences of DEPs and DEGs in the Integrative Analysis
4.2. Co-Expression of HSP70 and GST at Gene and Protein Levels under Heat Stress
4.3. Effective Pathways Involved in Response to Heat Stress
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Forward Primer Sequence (5′–3′) | Reverse Primer Sequence (5′–3′) |
---|---|---|
β-actin | GGCATCCACGAGACCACTT | CTCCTGCTTGCTGATCCACAT |
PIK3CA | GCTCAGCATAGGCATAAT | AGGAGATACGCCAAATAG |
CASP7 | GATGCTGGAGGACAGGGAT | TAGCGATGGTTGAAGATG |
HSP70 | CCAGCGTCAAGCAACCAA | TCAACTTCACGGCGGAGC |
HSP90 | AGGTCGTCCGAGTCCACA | GTCGCTGCTCTTCATTCC |
EIF6 | ATCCACGACACCCTACAAG | AGCCCAGTCATTCACTAACA |
ATPase | CGCCCATCCTCAACTCCT | CTCCACCAAACAGACCAATC |
V-ATPase | CCAGAACCAGCGTTACCA | CTCCTCTACCCTCAGCATC |
ALF | TAGTAGTTCCTGGCTGTTCCC | CCTCTATCGGCTGGTTCTG |
Functional Group/Gene | Description | Regulation | MT vs. CT | HT vs. CT | HT vs. MT |
---|---|---|---|---|---|
Heat shock protein family | |||||
HSP 70 | Heat shock protein 70 | Up | X | X | X |
HSP 90 | Heat shock protein 90 | Up | X | X | X |
Antioxidant system | |||||
SOD | Copper/zinc superoxide dismutase | Up | X | X | X |
GST | Glutathione S-transferase | Up | X | X | X |
GSH-Px | Glutathione peroxidase 3 | Up | X | ||
Energy metabolism | |||||
COX | Cytochrome c oxidase subunit | Up | X | ||
Down | X | X | |||
ND | NADH dehydrogenase subunit | Up | X | ||
Down | X | X | |||
CYC | Cytochrome c | Down | X | X | |
SDH | Succinate dehydrogenase | Down | X | X | |
IDH | Isocitrate dehydrogenase | Down | X | ||
CS | Citrate synthase | Down | X | ||
ATPase | ATP synthase | Down | X | X | |
V-ATPase | V-type proton ATPase | Down | X | X | |
Immune defense | |||||
CTL | LDLa domain-containing C-type lectin | Up | X | X | X |
CYP450 | Cytochrome P450 3A11 | Up | X | X | X |
IAP | Inhibitor of apoptosis protein | Up | X | X | X |
Chitinase | Chitinase | Up | X | X | X |
ARE | Apoptosis-resistant E3 ubiquitin protein | Up | X | X | X |
CASP3 | Caspase 3 | Up | X | X | |
CASP7 | Caspase 7 | Up | X | ||
CASP10 | Caspase 10 | Up | X | ||
ALF | Anti-lipopolysaccharide factor | Up | X | X |
Functional Pathway/Gene | Description | Regulation | MT vs. CT | HT vs. CT |
---|---|---|---|---|
Fc gamma R-mediated phagocytosis | ||||
PTPRC | Receptor-type tyrosine-protein phosphatase | Up | X | |
PIK3CA | Phosphoinositide-3 kinase | Up | X | |
RAC1 | Rho-related protein | Up | X | |
ECM–receptor interaction | ||||
Integrin α | Integrin alpha | Up | X | |
Integrin β | Integrin beta | Up | X | |
Regulation of actin cytoskeleton | ||||
Actin | Actin | Up | X | |
Integrin α | Integrin alpha | Up | X | |
Integrin β | Integrin beta | Up | X | |
PIK3CA | Phosphoinositide-3 kinase | Up | X | |
RAC1 | Rho-related protein | Up | X | |
Toll and Imd signaling pathway | ||||
Duox | Dual oxidase | Up | X | |
IAP | Inhibitor of apoptosis protein | Up | X | |
Relaxin signaling pathway | ||||
RXFP1 | Relaxin family peptide receptor 1 | Up | X | |
RXFP2 | Relaxin receptor 2 | Up | X | |
GNAO | Guanine nucleotide-binding protein G(o) subunit alpha isoform X2 | Up | X | |
NOS1 | Nitric oxide synthase | Up | X | |
PIK3CA | Phosphoinositide-3 kinase | Up | X | |
AC | Adenylate cyclase | Up | X | |
Relish | Relish | Up | X | |
PKC | Protein kinase C | Up | X | |
PLC | 1-Phosphatidylinositol 4,5-bisphosphate phosphodiesterase | Up | X | |
CREB | cAMP-Responsive element binding protein | Up | X | |
VEGF | Vascular endothelial growth factor | Up | X | |
JUN | Transcription factor AP-1 | Up | X | |
PKA | cAMP-Dependent protein kinase catalytic | Up | X | |
Renin–angiotensin system | ||||
NAP | Aminopeptidase N-like | Up | X | |
ACE | Angiotensin-converting enzyme | Up | X |
Functional Group/Gene | Description | Regulation | MT vs. CT | HT vs. CT | HT vs. MT |
---|---|---|---|---|---|
Heat shock protein family | |||||
HSP 70 | Heat shock protein 70 | Up | X | X | X |
HSP 60 | Heat shock protein 60 | Up | X | ||
Antioxidant system | |||||
CAT | Catalase | Up | X | X | |
Down | X | ||||
GST | Glutathione S-transferase | Up | X | X | |
Down | X | ||||
Energy metabolism | |||||
COX | Cytochrome c oxidase | Up | X | X | X |
ND | NADH dehydrogenase | Up | X | X | |
Down | X | ||||
CS | Citrate synthase | Up | X | X | |
Down | X | ||||
ATPase | ATP synthase | Up | X |
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Shen, C.; Feng, G.; Zhao, F.; Huang, X.; Wang, M.; Wang, H. Integration of Transcriptomics and Proteomics Analysis Reveals the Molecular Mechanism of Eriocheir sinensis Gills Exposed to Heat Stress. Antioxidants 2023, 12, 2020. https://doi.org/10.3390/antiox12122020
Shen C, Feng G, Zhao F, Huang X, Wang M, Wang H. Integration of Transcriptomics and Proteomics Analysis Reveals the Molecular Mechanism of Eriocheir sinensis Gills Exposed to Heat Stress. Antioxidants. 2023; 12(12):2020. https://doi.org/10.3390/antiox12122020
Chicago/Turabian StyleShen, Chenchen, Guangpeng Feng, Feng Zhao, Xiaorong Huang, Min Wang, and Haihua Wang. 2023. "Integration of Transcriptomics and Proteomics Analysis Reveals the Molecular Mechanism of Eriocheir sinensis Gills Exposed to Heat Stress" Antioxidants 12, no. 12: 2020. https://doi.org/10.3390/antiox12122020