Hydrogen Sulfide Donor GYY4137 Rescues NRF2 Activation in Respiratory Syncytial Virus Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. RSV Preparation
2.2. Primary Cells Culture Conditions and RSV Infection
2.3. Treatments
2.4. Western Blot
2.5. Reverse Transcription—Quantitative Polymerase Chain Reaction (RT-qPCR)
2.6. NRF2 Ubiquitination (Immunoprecipitation—Western Blot)
2.7. Histone Deacetylase (HDAC) Activity
2.8. Statistical Analysis
3. Results
3.1. H2S Donor GYY4137 Rescues NRF2 Levels and NRF2-Dependent Gene Expression in RSV Infection
3.2. H2S Donor GYY4137 Downregulates KEAP1 in RSV Infection
3.3. H2S Donor GYY4137 Restores NRF2 Ubiquitination and Does Not Affect HDAC Activity in RSV Infection
3.4. H2S Donor GYY4137 Downregulates PML in RSV Infection
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Haas de Mello, A.; Liu, T.; Garofalo, R.P.; Casola, A. Hydrogen Sulfide Donor GYY4137 Rescues NRF2 Activation in Respiratory Syncytial Virus Infection. Antioxidants 2022, 11, 1410. https://doi.org/10.3390/antiox11071410
Haas de Mello A, Liu T, Garofalo RP, Casola A. Hydrogen Sulfide Donor GYY4137 Rescues NRF2 Activation in Respiratory Syncytial Virus Infection. Antioxidants. 2022; 11(7):1410. https://doi.org/10.3390/antiox11071410
Chicago/Turabian StyleHaas de Mello, Aline, Tianshuang Liu, Roberto P. Garofalo, and Antonella Casola. 2022. "Hydrogen Sulfide Donor GYY4137 Rescues NRF2 Activation in Respiratory Syncytial Virus Infection" Antioxidants 11, no. 7: 1410. https://doi.org/10.3390/antiox11071410