Antioxidant Activity, Metal Chelating Ability and DNA Protective Effect of the Hydroethanolic Extracts of Crocus sativus Stigmas, Tepals and Leaves
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Plant Material
2.3. Preparation of Plant Material
2.4. Preparation of Hydroethanolic Extracts
2.5. Animals
2.6. Determination of Total Polyphenols
2.7. Antioxidant Activity
2.7.1. Antiradical Activity by DPPH Method
2.7.2. Ferric Reducing Antioxidant Power (FRAP)
2.7.3. Bleaching Test for β-Carotene
2.8. Metal Chelating Power
2.8.1. Iron Chelation Test
2.8.2. Copper Chelation Test
2.9. DNA Protective Effect
2.9.1. Collection and Treatment of Cells
- -
- Genotoxic effect
- -
- DNA-protective effect
- -
- Positive control
- -
- Negative control
2.9.2. Comet Assay
2.9.3. Microscopic Observation
2.10. Statistical Analysis
3. Results
3.1. Total Polyphenol Content
3.2. Antioxidant Activity
3.2.1. Antiradical Activity by DPPH Method
3.2.2. Effect on the Ferric Reducing Antioxidant Power
3.2.3. Effect on the Bleaching of β-Carotene
3.3. Metal Chelating Power
3.3.1. Iron Chelating Power
3.3.2. Copper Chelating Power
3.4. Genotoxic Effect on Rat Leukocytes
3.5. DNA-Protective Effect in Streptozotocin-Intoxicated Leukocytes
3.6. DNA-Protective Effect in Alloxan-Intoxicated Leukocytes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | Polyphenol Content (µg GA eq/mg Extract) |
---|---|
STG | (34.41 ± 1.09) |
TPL | (64.66 ± 0.20) |
LV | (38.56 ± 0.34) |
Sample | IC50 (µg/mL) |
---|---|
STG | 1554.37 ± 299.09 |
TPL | 80.73 ± 0.71 |
LV | 101.50 ± 1.55 |
Asc.A | 2.50 ± 0.20 |
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Ouahhoud, S.; Khoulati, A.; Kadda, S.; Bencheikh, N.; Mamri, S.; Ziani, A.; Baddaoui, S.; Eddabbeh, F.-E.; Lahmass, I.; Benabbes, R.; et al. Antioxidant Activity, Metal Chelating Ability and DNA Protective Effect of the Hydroethanolic Extracts of Crocus sativus Stigmas, Tepals and Leaves. Antioxidants 2022, 11, 932. https://doi.org/10.3390/antiox11050932
Ouahhoud S, Khoulati A, Kadda S, Bencheikh N, Mamri S, Ziani A, Baddaoui S, Eddabbeh F-E, Lahmass I, Benabbes R, et al. Antioxidant Activity, Metal Chelating Ability and DNA Protective Effect of the Hydroethanolic Extracts of Crocus sativus Stigmas, Tepals and Leaves. Antioxidants. 2022; 11(5):932. https://doi.org/10.3390/antiox11050932
Chicago/Turabian StyleOuahhoud, Sabir, Amine Khoulati, Salma Kadda, Noureddine Bencheikh, Samira Mamri, Anas Ziani, Sanae Baddaoui, Fatima-Ezzahra Eddabbeh, Iliass Lahmass, Redouane Benabbes, and et al. 2022. "Antioxidant Activity, Metal Chelating Ability and DNA Protective Effect of the Hydroethanolic Extracts of Crocus sativus Stigmas, Tepals and Leaves" Antioxidants 11, no. 5: 932. https://doi.org/10.3390/antiox11050932
APA StyleOuahhoud, S., Khoulati, A., Kadda, S., Bencheikh, N., Mamri, S., Ziani, A., Baddaoui, S., Eddabbeh, F. -E., Lahmass, I., Benabbes, R., Addi, M., Hano, C., Asehraou, A., & Saalaoui, E. (2022). Antioxidant Activity, Metal Chelating Ability and DNA Protective Effect of the Hydroethanolic Extracts of Crocus sativus Stigmas, Tepals and Leaves. Antioxidants, 11(5), 932. https://doi.org/10.3390/antiox11050932