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Mitochondrial Sirtuins in Reproduction
Article

Mitochondria Content and Activity Are Crucial Parameters for Bull Sperm Quality Evaluation

1
Department of Genetics and Animal Breeding, Faculty of Veterinary Medicine and Animal Sciences, Poznan University of Life Sciences, Wolynska 33, 60-637 Poznan, Poland
2
Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Carla Tatone and Giovanna Di Emidio
Antioxidants 2021, 10(8), 1204; https://doi.org/10.3390/antiox10081204
Received: 17 June 2021 / Revised: 9 July 2021 / Accepted: 20 July 2021 / Published: 27 July 2021
(This article belongs to the Special Issue Mitochondria Biology in Reproductive Function)
Standard sperm evaluation parameters do not enable predicting their ability to survive cryopreservation. Mitochondria are highly prone to suffer injuries during freezing, and any abnormalities in their morphology or function are reflected by a decline of sperm quality. Our work focused on describing a link between the number and the activity of mitochondria, with an aim to validate its applicability as a biomarker of bovine sperm quality. Cryopreserved sperm collected from bulls with high (group 1) and low (group 2) semen quality was separated by swim up. The spermatozoa of group 1 overall retained more mitochondria (MitoTrackerGreen) and mtDNA copies, irrespective of the fraction. Regardless of the initial ejaculate quality, the motile sperm contained significantly more mitochondria and mtDNA copies. The same trend was observed for mitochondrial membrane potential (ΔΨm, JC-1), where motile sperm displayed high ΔΨm. These results stay in agreement with transcript-level evaluation (real-time polymerase chain reaction, PCR) of antioxidant enzymes (PRDX1, SOD1, GSS), which protect cells from the reactive oxygen species. An overall higher level of glutathione synthetase (GSS) mRNA was noted in group 1 bulls, suggesting higher ability to counteract free radicals. No differences were noted between basal oxygen consumption rate (OCR) (Seahorse XF Agilent) and ATP-linked respiration for group 1 and 2 bulls. In conclusion, mitochondrial content and activity may be used as reliable markers for bovine sperm quality evaluation. View Full-Text
Keywords: spermatozoa; sperm motility; computer-assisted sperm analysis (CASA); mitochondria content and membrane potential; gene expression; oxygen consumption rate (OCR); Seahorse XF ATP Agilent spermatozoa; sperm motility; computer-assisted sperm analysis (CASA); mitochondria content and membrane potential; gene expression; oxygen consumption rate (OCR); Seahorse XF ATP Agilent
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MDPI and ACS Style

Madeja, Z.E.; Podralska, M.; Nadel, A.; Pszczola, M.; Pawlak, P.; Rozwadowska, N. Mitochondria Content and Activity Are Crucial Parameters for Bull Sperm Quality Evaluation. Antioxidants 2021, 10, 1204. https://doi.org/10.3390/antiox10081204

AMA Style

Madeja ZE, Podralska M, Nadel A, Pszczola M, Pawlak P, Rozwadowska N. Mitochondria Content and Activity Are Crucial Parameters for Bull Sperm Quality Evaluation. Antioxidants. 2021; 10(8):1204. https://doi.org/10.3390/antiox10081204

Chicago/Turabian Style

Madeja, Zofia E., Marta Podralska, Agnieszka Nadel, Marcin Pszczola, Piotr Pawlak, and Natalia Rozwadowska. 2021. "Mitochondria Content and Activity Are Crucial Parameters for Bull Sperm Quality Evaluation" Antioxidants 10, no. 8: 1204. https://doi.org/10.3390/antiox10081204

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