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Article

Peroxiporins Are Induced upon Oxidative Stress Insult and Are Associated with Oxidative Stress Resistance in Colon Cancer Cell Lines

1
Ruđer Bošković Institute, Bijenička 54, HR-10000 Zagreb, Croatia
2
Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal
*
Authors to whom correspondence should be addressed.
Academic Editor: Stanley Omaye
Antioxidants 2021, 10(11), 1856; https://doi.org/10.3390/antiox10111856
Received: 26 October 2021 / Revised: 19 November 2021 / Accepted: 20 November 2021 / Published: 22 November 2021
(This article belongs to the Special Issue Redox Signaling and Nrf2 in Cancers)
Oxidative stress can induce genetic instability and change cellular processes, resulting in colorectal cancer. Additionally, adaptation of oxidative defense causes therapy resistance, a major obstacle in successful cancer treatment. Peroxiporins are aquaporin membrane channels that facilitate H2O2 membrane permeation, crucial for regulating cell proliferation and antioxidative defense. Here, we investigated four colon cancer cell lines (Caco-2, HT-29, SW620, and HCT 116) for their sensitivity to H2O2, cellular antioxidative status, and ROS intracellular accumulation after H2O2 treatment. The expression of peroxiporins AQP1, AQP3, and AQP5 and levels of NRF2, the antioxidant transcription factor, and PPARγ, a transcription factor that regulates lipid metabolism, were evaluated before and after oxidative insult. Of the four tested cell lines, HT-29 was the most resistant and showed the highest expression of all tested peroxiporins and the lowest levels of intracellular ROS, without differences in GSH levels, catalase activity, nor NF2 and PPARγ levels. Caco-2 shows high expression of AQP3 and similar resistance as HT-29. These results imply that oxidative stress resistance can be obtained by several mechanisms other than the antioxidant defense system. Regulation of intracellular ROS through modulation of peroxiporin expression may represent an additional strategy to target the therapy resistance of cancer cells. View Full-Text
Keywords: aquaporin; colon cancer cell line; oxidative stress; NRF2 aquaporin; colon cancer cell line; oxidative stress; NRF2
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MDPI and ACS Style

Čipak Gašparović, A.; Milković, L.; Rodrigues, C.; Mlinarić, M.; Soveral, G. Peroxiporins Are Induced upon Oxidative Stress Insult and Are Associated with Oxidative Stress Resistance in Colon Cancer Cell Lines. Antioxidants 2021, 10, 1856. https://doi.org/10.3390/antiox10111856

AMA Style

Čipak Gašparović A, Milković L, Rodrigues C, Mlinarić M, Soveral G. Peroxiporins Are Induced upon Oxidative Stress Insult and Are Associated with Oxidative Stress Resistance in Colon Cancer Cell Lines. Antioxidants. 2021; 10(11):1856. https://doi.org/10.3390/antiox10111856

Chicago/Turabian Style

Čipak Gašparović, Ana, Lidija Milković, Claudia Rodrigues, Monika Mlinarić, and Graça Soveral. 2021. "Peroxiporins Are Induced upon Oxidative Stress Insult and Are Associated with Oxidative Stress Resistance in Colon Cancer Cell Lines" Antioxidants 10, no. 11: 1856. https://doi.org/10.3390/antiox10111856

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