Exploring the Leukemogenic Potential of GATA-1S, the Shorter Isoform of GATA-1: Novel Insights into Mechanisms Hampering Respiratory Chain Complex II Activity and Limiting Oxidative Phosphorylation Efficiency
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Transient Transfection
2.3. Protein Extraction
2.4. Western Blot Analysis
2.5. Total RNA Extraction
2.6. Real-Time PCR Analysis
2.7. Measurement of the Enzymatic Activity of Complex II Succinate-Ubiquinone Oxidoreductase
2.8. Seahorse Assay for Measurement of Cellular Respiration
2.9. Mitochondrial Mass Measurement
2.10. AML Patient Samples
2.11. Statistical Analysis
3. Results
3.1. Correlation between GATA-1 Isoforms and Expression Levels of SDHC Isoforms
3.2. Effects of GATA-1 Isoforms on Mitochondrial Metabolism
3.2.1. Measurement of SQR Activity
3.2.2. Evaluation of Cellular Energy Metabolism
3.3. Effects of GATA-1 Isoforms on Glutathione (GSH) Biosynthesis
3.4. Expression Levels of SDHC ASV Isoforms in an AML Patient
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Transcript | Accession Number | Primer | Sequence 5′-3′ | Amplicon Size |
---|---|---|---|---|
SDHC | NG_012767.1 | For 1 | CACTTCCGTCCAGACCGGA | 100 bp |
Rev 1 | CTGATACAGAGCTGAGGGCTAA | |||
SDHC full-length | NM_003001.5 | For 2 | TCTGTATCAGAAATGCTGTTCC | 183 bp |
Rev 2 | GAGACCCCTGCACTCAAAGC | |||
SDHC Δ 3 ASV | NM_001035512.2 (AB211234.1) | For 3 | GCTCTGTATCAGAAATTGGTCT | 250 bp |
Rev 3 | GTCCCACATCAAGTGTCGGA | |||
SDHC Δ 5 ASV | NM_001035511.2 (AB211235.1) | For 2 | TCTGTATCAGAAATGCTGTTCC | 187 bp |
Rev 4 | GGTCCCACATCTGCACTCAA | |||
HIF-1α | NM_001530.4 | For | TCCAAGAAGCCCTAACGTGT | 179 bp |
Rev | TGATCGTCTGGCTGCTGTAA | |||
GAPDH | NM_002046.7 | For | GAGCCACATCGCTCAGACAC | 116 bp |
Rev | GGCAACAATATCCACTTTACCA |
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Trombetti, S.; Sessa, R.; Catapano, R.; Rinaldi, L.; Lo Bianco, A.; Feliciello, A.; Izzo, P.; Grosso, M. Exploring the Leukemogenic Potential of GATA-1S, the Shorter Isoform of GATA-1: Novel Insights into Mechanisms Hampering Respiratory Chain Complex II Activity and Limiting Oxidative Phosphorylation Efficiency. Antioxidants 2021, 10, 1603. https://doi.org/10.3390/antiox10101603
Trombetti S, Sessa R, Catapano R, Rinaldi L, Lo Bianco A, Feliciello A, Izzo P, Grosso M. Exploring the Leukemogenic Potential of GATA-1S, the Shorter Isoform of GATA-1: Novel Insights into Mechanisms Hampering Respiratory Chain Complex II Activity and Limiting Oxidative Phosphorylation Efficiency. Antioxidants. 2021; 10(10):1603. https://doi.org/10.3390/antiox10101603
Chicago/Turabian StyleTrombetti, Silvia, Raffaele Sessa, Rosa Catapano, Laura Rinaldi, Alessandra Lo Bianco, Antonio Feliciello, Paola Izzo, and Michela Grosso. 2021. "Exploring the Leukemogenic Potential of GATA-1S, the Shorter Isoform of GATA-1: Novel Insights into Mechanisms Hampering Respiratory Chain Complex II Activity and Limiting Oxidative Phosphorylation Efficiency" Antioxidants 10, no. 10: 1603. https://doi.org/10.3390/antiox10101603