Bacillus amyloliquefaciens SC06 Induced AKT–FOXO Signaling Pathway-Mediated Autophagy to Alleviate Oxidative Stress in IPEC-J2 Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. BaSC06 Bacterial Strain Preparation
2.2. IPEC-J2 Cell Culture
2.3. Establishing Oxidative Stress Model in IPEC-J2 Cells
2.4. ROS Generation Analysis
2.5. Detection of Antioxidant Capacities
2.6. Apoptosis Cell Analysis by TUNEL Assay
2.7. Annexin V-FITC/PI Apoptosis Assay
2.8. Detection of Caspase-3 Activity
2.9. FOXO3a siRNA and Transfection
2.10. Western Blotting
2.11. Immunofluorescence Analysis
2.12. RNA Extractions and Quantitative Real-Time PCR (qPCR) Analysis
2.13. RNA Extraction and RNA-Seq Analysis
2.14. Statistical Analysis
3. Results
3.1. Establishment of Oxidative Stress Model Induced by Diquat in IPEC-J2 Cells
3.2. BaSC06 Alleviated Oxidative Stress Induced by DQ in IPEC-J2 Cells
3.3. BaSC06 Alleviated Oxidative Stress-Induced Apoptosis in IPEC-J2 Cells
3.4. BaSC06 Triggered Autophagy during Oxidative Stress in IPEC-J2 Cells
3.5. KEGG Pathway Analysis of DEGs
3.6. BaSC06 Can Regulate the Transcriptional Activity of FOXO3 Transcription Factor in IPEC-J2 Cells
3.7. BaSC06 Mediated Autophagy by the AKT–FOXO Signaling Pathway Independent of mTOR
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name. | Access No. | Sequences | |
---|---|---|---|
Sense(5′-3′) | Antisense(5′-3′) | ||
sscFOXO3a-608 | NM_001135959.1 | CCGGCUGGAAGAACUCUAUTT | AUAGAGUUCUUCCAGCCGGTT |
sscFOXO3a-1115 | NM_001135959.1 | CCGGAACCAUGAAUCUCAATT | UUGAGAUUCAUGGUUCCGGTT |
sscFOXO3a-1928 | NM_001135959.1 | CCCUCAUCUCCACACAGAATT | UUCUGUGUGGAGAUGAGGGTT |
Negative Control | NM_001135959.1 | UUCUCCGAACGUGUCACGUTT | ACGUGACACGUUCGGAGAATT |
Gene | Access No. | Primers Sequence | Length |
---|---|---|---|
GAPDH | NM_001206359.1 | F: CGGAGTGAACGGATTTGGC | 248 |
R: CACCCCATTTGATGTTGGCG | |||
ATG5 | NM_001037152.2 | F: AGTCAACCCTCCAATACCCAG | 299 |
R: TGTGGCCCTCTCTAGGTTTCT | |||
ATG12 | NM_001190282.1 | F: AGGTTGGATACCCGCCTACT | 111 |
R: ACTTGGTTGGAGCAATCT | |||
ATG16L1 | NM_001190272.1 | F: CTGCCAGTCGAACAGGATGA | 166 |
R: AGCGCCTCCCAAAGATATTAGT | |||
ATG8 | NM_001190288.1 | F: CCACCTTCCCACTCAGCTTT | 187 |
R: GTGTATCCTACCTTCCCCGC | |||
ATG14 | XM_001924990.5 | F: GCTTACACTGGACACCGCTA | 125 |
R: CCCCACGGCTTAACCTCTTT | |||
Caspase-8 | NM_001031779.2 | F: CCAGGATTTGCCTCCGGTTA | 108 |
R: GCCAGGTCATCACTGTCCAA |
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Tang, L.; Zeng, Z.; Zhou, Y.; Wang, B.; Zou, P.; Wang, Q.; Ying, J.; Wang, F.; Li, X.; Xu, S.; et al. Bacillus amyloliquefaciens SC06 Induced AKT–FOXO Signaling Pathway-Mediated Autophagy to Alleviate Oxidative Stress in IPEC-J2 Cells. Antioxidants 2021, 10, 1545. https://doi.org/10.3390/antiox10101545
Tang L, Zeng Z, Zhou Y, Wang B, Zou P, Wang Q, Ying J, Wang F, Li X, Xu S, et al. Bacillus amyloliquefaciens SC06 Induced AKT–FOXO Signaling Pathway-Mediated Autophagy to Alleviate Oxidative Stress in IPEC-J2 Cells. Antioxidants. 2021; 10(10):1545. https://doi.org/10.3390/antiox10101545
Chicago/Turabian StyleTang, Li, Zihan Zeng, Yuanhao Zhou, Baikui Wang, Peng Zou, Qi Wang, Jiafu Ying, Fei Wang, Xiang Li, Shujie Xu, and et al. 2021. "Bacillus amyloliquefaciens SC06 Induced AKT–FOXO Signaling Pathway-Mediated Autophagy to Alleviate Oxidative Stress in IPEC-J2 Cells" Antioxidants 10, no. 10: 1545. https://doi.org/10.3390/antiox10101545