Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (Triticum aestivum L.) Plants
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Growth Conditions
2.2. Leaf Crude Extracts for Enzymatic Assays
2.3. Glucose (Glc) Content
2.4. NO, H2S and H2O2 Content
2.5. Lipid Peroxidation
2.6. Non-Enzymatic Antioxidant Components
2.7. Enzymatic Antioxidant Systems
2.8. Photosynthetic Parameters
2.9. Leaf Area and Dry Mass Determination
2.10. Statistical Analysis
3. Results
4. Discussion
4.1. Heat Triggers Severe Stress and Glucose (Glc) Accumulation Which are Significantly Palliated by the Simultaneous Exogenous Application of NO and H2S
4.2. HS Triggers the Content of Endogenous NO and H2S
4.3. NO and H2S Trigger a General Increase of Antioxidant System Which Palliate the Oxidative Stress induced by HS
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Treatment | H2O2 | Glc | PN | PDM |
---|---|---|---|---|
Control | 17.5 ± 0.51 c | 3.7 ± 0.18 c | 12.2 ± 0.791 b | 0.97 ± 0.031 b |
Heat stress | 39.4 ± 0.96 a | 5.9 ± 0.25 a | 7.1± 0.574 d | 0.44 ± 0.017 d |
Heat stress + SNP + NaHS | 9.3 ± 0.36 d | 3.0 ± 0.14 d | 18.2 ± 1.102 a | 1.61 ± 0.039 a |
Heat stress + SNP + NaHS + cPTIO | 19.8 ± 0.68 c | 3.9 ± 0.19 c | 11.3 ± 0.684 b | 0.82 ± 0.025 b |
Heat stress + SNP + NaHS + HT | 30.5 ± 0.81 b | 4.8 ± 0. 21 b | 9.7± 0.629 c | 0.64 ± 0.191 c |
Heat stress + SNP + NaHS + cPTIO + HT | 41.6 ± 0.99 a | 5.7 ± 0.24 a | 7.9± 0.628 d | 0.49 ± 0.018 d |
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Iqbal, N.; Umar, S.; Khan, N.A.; Corpas, F.J. Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (Triticum aestivum L.) Plants. Antioxidants 2021, 10, 108. https://doi.org/10.3390/antiox10010108
Iqbal N, Umar S, Khan NA, Corpas FJ. Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (Triticum aestivum L.) Plants. Antioxidants. 2021; 10(1):108. https://doi.org/10.3390/antiox10010108
Chicago/Turabian StyleIqbal, Noushina, Shahid Umar, Nafees A. Khan, and Francisco J. Corpas. 2021. "Nitric Oxide and Hydrogen Sulfide Coordinately Reduce Glucose Sensitivity and Decrease Oxidative Stress via Ascorbate-Glutathione Cycle in Heat-Stressed Wheat (Triticum aestivum L.) Plants" Antioxidants 10, no. 1: 108. https://doi.org/10.3390/antiox10010108