Serology in the Digital Age: Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays
Abstract
:1. Introduction
2. Materials and Methods
2.1. Megapeptides
2.2. Other Reagents
2.3. Coupling of Peptides to Magnetic Luminex Beads
2.4. Suspension Multiplex Immunoassay (SMIA)
3. Results
3.1. Mimicking Ebola Glycoprotein Antigenicity with A Megapeptide
3.2. Mimicking Human Herpesvirus 7 Glycoprotein B Antigenicity Using Megapeptides
3.3. Mimicking JC Polyoma Virus (JCV) Capsid Protein VP1 Antigenicity Using Megapeptides
4. Discussion
4.1. Serology in the Digital Age
4.2. Megapeptides for Detection of Ebola, HHV7, and JCV Antibodies
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Rizwan, M.; Rönnberg, B.; Cistjakovs, M.; Lundkvist, Å.; Pipkorn, R.; Blomberg, J. Serology in the Digital Age: Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays. Microarrays 2016, 5, 22. https://doi.org/10.3390/microarrays5030022
Rizwan M, Rönnberg B, Cistjakovs M, Lundkvist Å, Pipkorn R, Blomberg J. Serology in the Digital Age: Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays. Microarrays. 2016; 5(3):22. https://doi.org/10.3390/microarrays5030022
Chicago/Turabian StyleRizwan, Muhammad, Bengt Rönnberg, Maksims Cistjakovs, Åke Lundkvist, Rudiger Pipkorn, and Jonas Blomberg. 2016. "Serology in the Digital Age: Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays" Microarrays 5, no. 3: 22. https://doi.org/10.3390/microarrays5030022
APA StyleRizwan, M., Rönnberg, B., Cistjakovs, M., Lundkvist, Å., Pipkorn, R., & Blomberg, J. (2016). Serology in the Digital Age: Using Long Synthetic Peptides Created from Nucleic Acid Sequences as Antigens in Microarrays. Microarrays, 5(3), 22. https://doi.org/10.3390/microarrays5030022