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Living Cell Microarrays: An Overview of Concepts

Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, Hannover 30167, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Holger Erfle
Microarrays 2016, 5(2), 11; https://doi.org/10.3390/microarrays5020011
Received: 30 March 2016 / Revised: 9 May 2016 / Accepted: 11 May 2016 / Published: 26 May 2016
(This article belongs to the Special Issue Cell-Based Microarrays)
Living cell microarrays are a highly efficient cellular screening system. Due to the low number of cells required per spot, cell microarrays enable the use of primary and stem cells and provide resolution close to the single-cell level. Apart from a variety of conventional static designs, microfluidic microarray systems have also been established. An alternative format is a microarray consisting of three-dimensional cell constructs ranging from cell spheroids to cells encapsulated in hydrogel. These systems provide an in vivo-like microenvironment and are preferably used for the investigation of cellular physiology, cytotoxicity, and drug screening. Thus, many different high-tech microarray platforms are currently available. Disadvantages of many systems include their high cost, the requirement of specialized equipment for their manufacture, and the poor comparability of results between different platforms. In this article, we provide an overview of static, microfluidic, and 3D cell microarrays. In addition, we describe a simple method for the printing of living cell microarrays on modified microscope glass slides using standard DNA microarray equipment available in most laboratories. Applications in research and diagnostics are discussed, e.g., the selective and sensitive detection of biomarkers. Finally, we highlight current limitations and the future prospects of living cell microarrays. View Full-Text
Keywords: living cell microarrays (LCMAs); transfected cell microarrays; affinity-based arrays; cell attachment and stimulation; 3D cell constructs; microfluidic devices; organ-on-a-chip (OOC) living cell microarrays (LCMAs); transfected cell microarrays; affinity-based arrays; cell attachment and stimulation; 3D cell constructs; microfluidic devices; organ-on-a-chip (OOC)
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MDPI and ACS Style

Jonczyk, R.; Kurth, T.; Lavrentieva, A.; Walter, J.-G.; Scheper, T.; Stahl, F. Living Cell Microarrays: An Overview of Concepts. Microarrays 2016, 5, 11.

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