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Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line

1
Institute of Pharmacology, Catholic University Medical School, L.go F Vito 1, 00168 Rome, Italy
2
Fondazione Policlinico Universitario Agostino Gemelli, L.go F. Vito 1, 00168 Rome, Italy
*
Author to whom correspondence should be addressed.
These authors have equally contributed to the manuscript.
Brain Sci. 2019, 9(5), 111; https://doi.org/10.3390/brainsci9050111
Received: 1 April 2019 / Revised: 6 May 2019 / Accepted: 13 May 2019 / Published: 15 May 2019
(This article belongs to the Section Neuroglia)
PDF [1864 KB, uploaded 15 May 2019]

Abstract

: The characterization of human microglia has been hampered by poor availability of human cell sources. However, microglia is involved in the physiopathology of multiple sclerosis, Alzheimer’s disease, Parkinson’s disease, HIV dementia, retinal degenerative diseases, cancer, and many other conditions. Therefore, there is an important need to have experimental paradigms of human microglia characterized and usable to study the role of microglia in the different pathologies in which it is involved. In the present work, we carried out an extensive characterization of Immortalized Human Microglia—SV40 cell line (IMhu), marketed by Applied Biological Material. The functional response of IMhu to a large variety of stimuli was studied. In particular, we investigated morphology, mortality, and changes in the production of different cytokines and chemokines, both under basal conditions and after stimulation. Moreover, western blotting analysis was conducted on phospho-mTOR (Ser 2448) and downstream parameters, p-P70S6K and 4EBP1, in order to understand if IMhu can be used for evaluations of mTOR pathway. In conclusion, IMhu cells proved to be a useful experimental model to investigate the physiopathology of inflammatory disease that involved microglia cells, including pathological conditions that involved the mTOR pathway.
Keywords: human microglia; cytokines; M1 phenotype; mTOR human microglia; cytokines; M1 phenotype; mTOR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Chiavari, M.; Ciotti, G.M.P.; Navarra, P.; Lisi, L. Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line. Brain Sci. 2019, 9, 111.

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