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Article

A Multidisciplinary Approach Reveals an Age-Dependent Expression of a Novel Bioactive Peptide, Already Involved in Neurodegeneration, in the Postnatal Rat Forebrain

1
Neuro-Bio Ltd., Culham Science Centre, Building F5, Abingdon OX14 3DB, UK
2
School of Physiology, Pharmacology and Neuroscience, Faculty of Biomedical Sciences, University of Bristol, Bristol BS8 1TD, UK
3
School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Brain Sci. 2018, 8(7), 132; https://doi.org/10.3390/brainsci8070132
Received: 29 May 2018 / Revised: 4 July 2018 / Accepted: 6 July 2018 / Published: 10 July 2018
The basal forebrain has received much attention due to its involvement in multiple cognitive functions, but little is known about the basic neuronal mechanisms underlying its development, nor those mediating its primary role in Alzheimer’s disease. We have previously suggested that a novel 14-mer peptide, ‘T14’, could play a pivotal role in Alzheimer’s disease, via reactivation of a developmental signaling pathway. In this study, we have characterized T14 in the context of post-natal rat brain development, using a combination of different techniques. Ex-vivo rat brain slices containing the basal forebrain, at different stages of development, were used to investigate large-scale neuronal network activity in real time with voltage-sensitive dye imaging. Subsequent Western blot analysis revealed the expression profile of endogenous T14, its target alpha7 nicotinic receptor and the familiar markers of Alzheimer’s: amyloid beta and phosphorylated Tau. Results indicated maximal neuronal activity at the earliest ages during development, reflected in a concomitant profile of T14 peptide levels and related proteins. In conclusion, these findings show that the peptide, already implicated in neurodegenerative events, has an age-dependent expression, suggesting a possible contribution to the physiological mechanisms underlying brain maturation. View Full-Text
Keywords: development; basal forebrain; AChE-derived peptide; nicotinic receptors; optical imaging; Alzheimer’s disease development; basal forebrain; AChE-derived peptide; nicotinic receptors; optical imaging; Alzheimer’s disease
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MDPI and ACS Style

Ferrati, G.; Brai, E.; Stuart, S.; Marino, C.; Greenfield, S.A. A Multidisciplinary Approach Reveals an Age-Dependent Expression of a Novel Bioactive Peptide, Already Involved in Neurodegeneration, in the Postnatal Rat Forebrain. Brain Sci. 2018, 8, 132. https://doi.org/10.3390/brainsci8070132

AMA Style

Ferrati G, Brai E, Stuart S, Marino C, Greenfield SA. A Multidisciplinary Approach Reveals an Age-Dependent Expression of a Novel Bioactive Peptide, Already Involved in Neurodegeneration, in the Postnatal Rat Forebrain. Brain Sciences. 2018; 8(7):132. https://doi.org/10.3390/brainsci8070132

Chicago/Turabian Style

Ferrati, Giovanni, Emanuele Brai, Skye Stuart, Celia Marino, and Susan A. Greenfield. 2018. "A Multidisciplinary Approach Reveals an Age-Dependent Expression of a Novel Bioactive Peptide, Already Involved in Neurodegeneration, in the Postnatal Rat Forebrain" Brain Sciences 8, no. 7: 132. https://doi.org/10.3390/brainsci8070132

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