MicroRNA-223 Attenuates Stretch-Injury-Induced Apoptosis in Brain Microvascular Endothelial Cells by Regulating RhoB Expression
Abstract
1. Introduction
2. Method
2.1. Endothelial Cells Culture
2.2. Mechanical Cell Injury
2.3. Cell Transfection
2.4. Immunostaining
2.5. Relative Real-Time Polymerase Chain Reaction
- miR-223-F: 5′-TGTCAGTTTGTCAAATA-3′;
- miR-223-R: 5′-GTGCAGGGTCCGAGGT-3′;
- U6-F: 5′CGCTTCGGCAGCACATATAC-3′;
- U6-R: 5′- AAATATGGAACGCT-TCACGA-3′;
- RhoB-F:5′-TCGTGTTCAGTAAGGACGAG-3′;
- RhoB-R:5′-ACTTCTCGGGGATGTTCTC-3′;
- GAPDH-F: 5′- TGAGGCCGGTGCTGAGTATGT -3′;
- GAPDH-R: 5′- CAGTCTTCTGGGTGGCAGTGAT-3′;
2.6. Western Blot Analysis
2.7. Flow Cytometry
2.8. Statistical Analysis
3. Result
3.1. The Expression of miR-223 Increased after SI
3.2. miR-223 Was Overexpressed in bEnd.3 Cells by Lentivirus Transfection
3.3. miR-223 Attenuates the Loss of Tight Junction Protein after SI in bEnd.3 Cells
3.4. miR-223 Inhibits Apoptosis of bEnd.3 Cells after Stretch Injury
3.5. miR-223 Modulated SI-Induced Caspase-3 Activation
3.6. MiR-223 Inhibited RhoB Expression, and Knockdown of RhoB Decreased Caspase-3 Activation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, Y.; Li, W.; Liu, Y.; Jiang, Y.; Wang, Y.; Xu, Z.; Cui, D.; Gao, L. MicroRNA-223 Attenuates Stretch-Injury-Induced Apoptosis in Brain Microvascular Endothelial Cells by Regulating RhoB Expression. Brain Sci. 2022, 12, 1157. https://doi.org/10.3390/brainsci12091157
Liu Y, Li W, Liu Y, Jiang Y, Wang Y, Xu Z, Cui D, Gao L. MicroRNA-223 Attenuates Stretch-Injury-Induced Apoptosis in Brain Microvascular Endothelial Cells by Regulating RhoB Expression. Brain Sciences. 2022; 12(9):1157. https://doi.org/10.3390/brainsci12091157
Chicago/Turabian StyleLiu, Yingliang, Wenjing Li, Yingxiu Liu, Yang Jiang, Yida Wang, Zhiming Xu, Daming Cui, and Liang Gao. 2022. "MicroRNA-223 Attenuates Stretch-Injury-Induced Apoptosis in Brain Microvascular Endothelial Cells by Regulating RhoB Expression" Brain Sciences 12, no. 9: 1157. https://doi.org/10.3390/brainsci12091157
APA StyleLiu, Y., Li, W., Liu, Y., Jiang, Y., Wang, Y., Xu, Z., Cui, D., & Gao, L. (2022). MicroRNA-223 Attenuates Stretch-Injury-Induced Apoptosis in Brain Microvascular Endothelial Cells by Regulating RhoB Expression. Brain Sciences, 12(9), 1157. https://doi.org/10.3390/brainsci12091157