Sex Differences in the Behavioural Aspects of the Cuprizone-Induced Demyelination Model in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Cuprizone-Induced Demyelination Model
2.3. Black Gold II Staining
2.4. Mechanical Sensitivity
2.5. Horizontal Bars
2.6. Passive Wire Hang
2.7. Motor Skill Sequence Testing
2.8. Elevated Zero Maze
2.9. Marble Burying
2.10. Z-Score Analysis
2.11. Statistical Analysis
3. Results
3.1. Cuprizone Administration Led to Increased Weight Loss in Male Mice Compared to Females
3.2. Cuprizone Administration Led to Demyelination in the Corpus Callosum
3.3. Cuprizone Administration Produced Mechanical Sensitivity in Both Male and Female Mice
3.4. Effects of Cuprizone Administration on Motor Coordination Were Significantly Greater in Male Mice Compare to Females
3.5. Cuprizone Administration Decreased Spontaneous Running Wheel Activity
3.6. Cuprizone Administration Increases Anxiety-Like Behaviours in Male Mice but Not in Female Mice
3.7. Cuprizone Administration Had the Most Severe Deficit in Males Administered the 0.3% Dose
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Paton, K.F.; Hong, S.; Biggerstaff, A.; Kivell, B.M. Sex Differences in the Behavioural Aspects of the Cuprizone-Induced Demyelination Model in Mice. Brain Sci. 2022, 12, 1687. https://doi.org/10.3390/brainsci12121687
Paton KF, Hong S, Biggerstaff A, Kivell BM. Sex Differences in the Behavioural Aspects of the Cuprizone-Induced Demyelination Model in Mice. Brain Sciences. 2022; 12(12):1687. https://doi.org/10.3390/brainsci12121687
Chicago/Turabian StylePaton, Kelly F., Sheein Hong, Andrew Biggerstaff, and Bronwyn M. Kivell. 2022. "Sex Differences in the Behavioural Aspects of the Cuprizone-Induced Demyelination Model in Mice" Brain Sciences 12, no. 12: 1687. https://doi.org/10.3390/brainsci12121687