Next Article in Journal
Effects of Transcranial Direct Current Stimulation (tDCS) on Go/NoGo Performance Using Food and Non-Food Stimuli in Patients with Prader–Willi Syndrome
Previous Article in Journal
HIV Infection and Related Mental Disorders
Article

5-Aminoisoquinolinone, a PARP-1 Inhibitor, Ameliorates Immune Abnormalities through Upregulation of Anti-Inflammatory and Downregulation of Inflammatory Parameters in T Cells of BTBR Mouse Model of Autism

Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
*
Author to whom correspondence should be addressed.
Brain Sci. 2021, 11(2), 249; https://doi.org/10.3390/brainsci11020249
Received: 11 December 2020 / Revised: 19 January 2021 / Accepted: 13 February 2021 / Published: 17 February 2021
(This article belongs to the Section Molecular and Cellular Neuroscience)
Autism spectrum disorder (ASD) covers a range of neurodevelopmental disorders involving impairments in communication and repetitive and stereotyped patterns of behavior and reciprocal social interaction. 5-Aminoisoquinolinone (5-AIQ), a PARP-1 inhibitor, has neuroprotective and anti-inflammatory effects. We investigated the influence of 5-AIQ-treatment in BTBR T+ Itpr3tf/J (BTBR) mice as an autism model and used flow cytometry to assess the effect of 5-AIQ on FOXP3, Helios, GATA3, IL-9, IL-10 and IL-17A production by CXCR6+ and CD4+ T cells in the spleen. We also confirmed the effect of 5-AIQ treatment on expression of FOXP3, Helios, GATA3, IL-17A, IL-10, and IL-9 mRNA and protein expression levels in the brain tissue by quantitative PCR and western blotting. Our results demonstrated that 5-AIQ-treated BTBR mice had significantly increased numbers of CXCR6+FOXP3+, CXCR6+IL-10+, and CXCR6+Helios+ cells and decreased numbers of CD4+GATA3+, CD4+IL-9+, and CD4+IL-17A+ cells as compared with those in untreated BTBR mice. Our results further demonstrated that treatment with 5-AIQ in BTBR mice increased expression for FOXP3, IL-10, and Helios, and decreased expression for GATA3, IL-17A, and IL-9 mRNA. Our findings support the hypotheses that 5-AIQ has promising novel therapeutic effects on neuroimmune dysfunction in autism and is associated with modulation of Treg and Th17 cells. View Full-Text
Keywords: autism; BTBR mice; 5-AIQ; cytokines; transcription factors; spleen and brain tissue autism; BTBR mice; 5-AIQ; cytokines; transcription factors; spleen and brain tissue
Show Figures

Figure 1

MDPI and ACS Style

Alhosaini, K.; Ansari, M.A.; Nadeem, A.; Bakheet, S.A.; Attia, S.M.; Alhazzani, K.; Albekairi, T.H.; Al-Mazroua, H.A.; Mahmood, H.M.; Ahmad, S.F. 5-Aminoisoquinolinone, a PARP-1 Inhibitor, Ameliorates Immune Abnormalities through Upregulation of Anti-Inflammatory and Downregulation of Inflammatory Parameters in T Cells of BTBR Mouse Model of Autism. Brain Sci. 2021, 11, 249. https://doi.org/10.3390/brainsci11020249

AMA Style

Alhosaini K, Ansari MA, Nadeem A, Bakheet SA, Attia SM, Alhazzani K, Albekairi TH, Al-Mazroua HA, Mahmood HM, Ahmad SF. 5-Aminoisoquinolinone, a PARP-1 Inhibitor, Ameliorates Immune Abnormalities through Upregulation of Anti-Inflammatory and Downregulation of Inflammatory Parameters in T Cells of BTBR Mouse Model of Autism. Brain Sciences. 2021; 11(2):249. https://doi.org/10.3390/brainsci11020249

Chicago/Turabian Style

Alhosaini, Khaled, Mushtaq A. Ansari, Ahmed Nadeem, Saleh A. Bakheet, Sabry M. Attia, Khalid Alhazzani, Thamer H. Albekairi, Haneen A. Al-Mazroua, Hafiz M. Mahmood, and Sheikh F. Ahmad. 2021. "5-Aminoisoquinolinone, a PARP-1 Inhibitor, Ameliorates Immune Abnormalities through Upregulation of Anti-Inflammatory and Downregulation of Inflammatory Parameters in T Cells of BTBR Mouse Model of Autism" Brain Sciences 11, no. 2: 249. https://doi.org/10.3390/brainsci11020249

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop