The Role of Neutrophil Extracellular Traps in Lipopolysaccharide-Induced Depression-like Behaviors in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Systemic LPS Administration
2.3. Neutrophil Extraction and Immunostaining
2.4. Immunohistochemistry (IHC)
2.5. ELISA
2.6. Quantitative RT–PCR
2.7. Behavioral Assay
2.7.1. Open Field Test (OFT)
2.7.2. Forced Swim Test (FST)
2.7.3. Tail Suspension Test (TST)
2.8. Statistical Analysis
3. Results
3.1. NETs Formation Is Induced in Mice with Depression-like Behaviors
3.2. Depression-like Behaviors Are Relieved in PAD4 KO Mice
3.3. Proinflammatory Cytokines in Plasma Is Decreased in PAD4 KO Mice
3.4. Proinflammatory Cytokines in Brain Is Reduced in PAD4 KO Mice
3.5. The Influence on Glia Cells Can Be Relieved in PAD4 KO Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kong, Y.; He, G.; Zhang, X.; Li, J. The Role of Neutrophil Extracellular Traps in Lipopolysaccharide-Induced Depression-like Behaviors in Mice. Brain Sci. 2021, 11, 1514. https://doi.org/10.3390/brainsci11111514
Kong Y, He G, Zhang X, Li J. The Role of Neutrophil Extracellular Traps in Lipopolysaccharide-Induced Depression-like Behaviors in Mice. Brain Sciences. 2021; 11(11):1514. https://doi.org/10.3390/brainsci11111514
Chicago/Turabian StyleKong, Yue, Guiqin He, Xiaolin Zhang, and Jin Li. 2021. "The Role of Neutrophil Extracellular Traps in Lipopolysaccharide-Induced Depression-like Behaviors in Mice" Brain Sciences 11, no. 11: 1514. https://doi.org/10.3390/brainsci11111514