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Appl. Sci. 2018, 8(9), 1572;

The Construction of an Engineered Bacterial Strain and Its Application in Accumulating Mercury from Wastewater

Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102, Hainan, China
The Key Lab of Hainan Banana Genetics and Breeding, Haikou 570102, Hainan, China
School of Forestry, Henan University of Science and Technology, Luoyang 471023, Henan, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 7 August 2018 / Revised: 27 August 2018 / Accepted: 3 September 2018 / Published: 6 September 2018
(This article belongs to the Section Applied Biosciences and Bioengineering)
Full-Text   |   PDF [1988 KB, uploaded 6 September 2018]   |  


To remove organic and inorganic mercury from wastewater, an engineered bacterial strain, BL21-7, was constructed that contained the artificial operon P16S-g10-merT-merP-merB1-merB2-ppk-rpsT. For BL21-7, the minimum inhibitory concentrations of mercuric chloride, methylmercury chloride and phenylmercury chloride in Luria-Bertani (LB) medium were 100 µmol/L, 60 µmol/L and 80 µmol/L, respectively. After being cultured in three media (liquid LB containing 80 µmol/L mercuric chloride, 40 µmol/L methylmercury chloride or 60 µmol/L phenylmercury chloride) for 72 h, the engineered bacteria accumulated up to 70.5 ± 1.5 µmol/L, 33.5 ± 3.2 µmol/L and 45.3 ± 3.7 µmol/L of mercury, respectively. In the presence of 10 µmol/L Cd2+, 10 µmol/L Pb2+ or 10 µmol/L Cu2+, the accumulation of mercurial derivatives by BL21-7 was not affected. BL21-7 could accumulate mercury well in media with pH values ranging from 5 to 8 and it could work well at temperatures from 25 °C to 37 °C. After BL21-7 was added to wastewater and cultured for 24 h, approximately 43.7% of the Hg in the wastewater was removed. View Full-Text
Keywords: engineering bacteria; mercury; accumulation engineering bacteria; mercury; accumulation

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Chang, S.; Wu, Z.; Sun, W.; Shu, H. The Construction of an Engineered Bacterial Strain and Its Application in Accumulating Mercury from Wastewater. Appl. Sci. 2018, 8, 1572.

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