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Vacuum Ultraviolet Single-Photon Postionization of Amino Acids

Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei 10617, Taiwan
Author to whom correspondence should be addressed.
Appl. Sci. 2018, 8(5), 699;
Received: 29 March 2018 / Revised: 25 April 2018 / Accepted: 27 April 2018 / Published: 2 May 2018
(This article belongs to the Special Issue Photoionization Mass Spectrometry)
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In this study, ultraviolet (UV) laser desorption and vacuum UV single-photon (VUV SP) postionization were performed to ionize and successfully analyze 20 common amino acids. The analytical merit and efficiency of the ionization was compared with those of conventional UV matrix-assisted laser desorption ionization (UV-MALDI). A VUV light source (118 nm) was generated from the ninth harmonic of a Q-switched Nd:YAG laser, and the photon number was determined to be larger than 1012 for each laser pulse in the ionization region. In general, the detection sensitivity of VUV-SP-postionization was 10–100 times higher than that of conventional UV-MALDI. In particular, the ion signal from VUV-SP-postionization was considerably larger than that from UV-MALDI for analytes with low proton affinity such as glycine. However, some fragmentation of intact ions was observed in VUV-SP-postionization. Quantitative analysis performed using a glycine/histidine mixture and tryptophan/phenylalanine mixture revealed that the dynamic range of VUV-SP-postionization was one order of magnitude larger than that of UV-MALDI, indicating that VUV-SP-postionization is suitable for the quantitative analysis of amino acids. View Full-Text
Keywords: photoionization; mass spectrometry; MALDI, VUV; postionization; photoionization; amino acid photoionization; mass spectrometry; MALDI, VUV; postionization; photoionization; amino acid

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Hsu, H.C.; Ni, C.-K. Vacuum Ultraviolet Single-Photon Postionization of Amino Acids. Appl. Sci. 2018, 8, 699.

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