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Appl. Sci. 2016, 6(11), 343;

Optimization of One-Step In Situ Transesterification Method for Accurate Quantification of EPA in Nannochloropsis gaditana

College of Chemical Engineering, Nanjing Forestry University, Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing 210037, China
Department of Chemical & Biomolecular Engineering, Johns Hopkins University, 3400 N. Charles St, Baltimore, MD 21218, USA
Author to whom correspondence should be addressed.
Academic Editor: Raed Abu-Reziq
Received: 27 September 2016 / Revised: 27 October 2016 / Accepted: 3 November 2016 / Published: 8 November 2016
(This article belongs to the Section Chemistry)
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Microalgae are a valuable source of lipid feedstocks for biodiesel and valuable omega-3 fatty acids. Nannochloropsis gaditana has emerged as a promising producer of eicosapentaenoic acid (EPA) due to its fast growth rate and high EPA content. In the present study, the fatty acid profile of Nannochloropsis gaditana was found to be naturally high in EPA and devoid of docosahexaenoic acid (DHA), thereby providing an opportunity to maximize the efficacy of EPA production. Using an optimized one-step in situ transesterification method (methanol:biomass = 90 mL/g; HCl 5% by vol.; 70 °C; 1.5 h), the maximum fatty acid methyl ester (FAME) yield of Nannochloropsis gaditana cultivated under rich condition was quantified as 10.04% ± 0.08% by weight with EPA-yields as high as 4.02% ± 0.17% based on dry biomass. The total FAME and EPA yields were 1.58- and 1.23-fold higher separately than that obtained using conventional two-step method (solvent system: methanol and chloroform). This one-step in situ method provides a fast and simple method to measure fatty acid methyl ester (FAME) yields and could serve as a promising method to generate eicosapentaenoic acid methyl ester from microalgae. View Full-Text
Keywords: EPA; FAME; one-step in situ transesterification; Nannochloropsis gaditana EPA; FAME; one-step in situ transesterification; Nannochloropsis gaditana

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Tang, Y.; Zhang, Y.; Rosenberg, J.N.; Betenbaugh, M.J.; Wang, F. Optimization of One-Step In Situ Transesterification Method for Accurate Quantification of EPA in Nannochloropsis gaditana. Appl. Sci. 2016, 6, 343.

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