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Open AccessArticle

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

1
Applied and Environmental Microbiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, Alice 5700, South Africa
2
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice 5700, South Africa
*
Author to whom correspondence should be addressed.
Academic Editor: Chih-Ching Huang
Appl. Sci. 2016, 6(10), 286; https://doi.org/10.3390/app6100286
Received: 21 July 2016 / Revised: 21 September 2016 / Accepted: 27 September 2016 / Published: 9 October 2016
Cellulase and xylanase are in high demand for application in several industrial processes, consequently necessitating the bioprospecting and manipulation of microbes for novel and greater enzyme productivity. This study reports on the optimal conditions for cellulase and xylanase production by a marine bacterial isolate from Nahoon beach sediment, via the classical process of one variable per time. Furthermore, the inducing effect of mono- and polysaccharides on enzyme production was investigated. The 16S rDNA gene sequence analysis clearly assigned the isolate to the genus Streptomyces, and was deposited at the GenBank under the accession number KU171373. Cellulase and xylanase production was optimal at the following conditions: pH 6 and 8, incubation temperature of 40 and 35 °C, and agitation speed of 100 and 150 rpm, respectively. Under optimum conditions, 0.26 U/mL and 18.54 U/mL activities were attained at 60 and 48 h with specific productivity of 205 and 7417 U/g for cellulase and xylanase, respectively. Xylanase production was induced by the entire mono- and polysaccharides tested, while cellulase production was induced by some. The results from this study signify the resourcefulness of the Streptomyces strain for production of cellulase and xylanase of industrial importance. View Full-Text
Keywords: cellulase; xylanase; optimization; saccharides; Streptomyces; activity; production cellulase; xylanase; optimization; saccharides; Streptomyces; activity; production
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MDPI and ACS Style

Fatokun, E.N.; Nwodo, U.U.; Okoh, A.I. Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species. Appl. Sci. 2016, 6, 286. https://doi.org/10.3390/app6100286

AMA Style

Fatokun EN, Nwodo UU, Okoh AI. Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species. Applied Sciences. 2016; 6(10):286. https://doi.org/10.3390/app6100286

Chicago/Turabian Style

Fatokun, Evelyn N.; Nwodo, Uchechukwu U.; Okoh, Anthony I. 2016. "Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species" Appl. Sci. 6, no. 10: 286. https://doi.org/10.3390/app6100286

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