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Open AccessFeature PaperArticle

An RNA Triangle with Six Ribozyme Units Can Promote a Trans-Splicing Reaction through Trimerization of Unit Ribozyme Dimers

1
Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 930-8555, Japan
2
Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan
3
Department of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8502, Japan
4
Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto 606-8502, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Tamaki Endoh and Chih-Ching Huang
Appl. Sci. 2021, 11(6), 2583; https://doi.org/10.3390/app11062583
Received: 29 November 2020 / Revised: 5 March 2021 / Accepted: 11 March 2021 / Published: 14 March 2021
(This article belongs to the Special Issue Nucleic Acids Conjugates for Biotechnological Applications)
Ribozymes are catalytic RNAs that are attractive platforms for the construction of nanoscale objects with biological functions. We designed a dimeric form of the Tetrahymena group I ribozyme as a unit structure in which two ribozymes were connected in a tail-to-tail manner with a linker element. We introduced a kink-turn motif as a bent linker element of the ribozyme dimer to design a closed trimer with a triangular shape. The oligomeric states of the resulting ribozyme dimers (kUrds) were analyzed biochemically and observed directly by atomic force microscopy (AFM). Formation of kUrd oligomers also triggered trans-splicing reactions, which could be monitored with a reporter system to yield a fluorescent RNA aptamer as the trans-splicing product. View Full-Text
Keywords: catalytic RNA; group I ribozyme; RNA nanostructure; RNA nanotechnology; RNA-protein complex; trans-splicing catalytic RNA; group I ribozyme; RNA nanostructure; RNA nanotechnology; RNA-protein complex; trans-splicing
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MDPI and ACS Style

Akagi, J.; Yamada, T.; Hidaka, K.; Fujita, Y.; Saito, H.; Sugiyama, H.; Endo, M.; Matsumura, S.; Ikawa, Y. An RNA Triangle with Six Ribozyme Units Can Promote a Trans-Splicing Reaction through Trimerization of Unit Ribozyme Dimers. Appl. Sci. 2021, 11, 2583. https://doi.org/10.3390/app11062583

AMA Style

Akagi J, Yamada T, Hidaka K, Fujita Y, Saito H, Sugiyama H, Endo M, Matsumura S, Ikawa Y. An RNA Triangle with Six Ribozyme Units Can Promote a Trans-Splicing Reaction through Trimerization of Unit Ribozyme Dimers. Applied Sciences. 2021; 11(6):2583. https://doi.org/10.3390/app11062583

Chicago/Turabian Style

Akagi, Junya; Yamada, Takahiro; Hidaka, Kumi; Fujita, Yoshihiko; Saito, Hirohide; Sugiyama, Hiroshi; Endo, Masayuki; Matsumura, Shigeyoshi; Ikawa, Yoshiya. 2021. "An RNA Triangle with Six Ribozyme Units Can Promote a Trans-Splicing Reaction through Trimerization of Unit Ribozyme Dimers" Appl. Sci. 11, no. 6: 2583. https://doi.org/10.3390/app11062583

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