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Analytical Pyrolysis of the Fungal Melanins from Ochroconis spp. Isolated from Lascaux Cave, France

1
Instituto de Recursos Naturales y Agrobiologia, IRNAS-CSIC, 41012 Sevilla, Spain
2
Section for Genetics and Evolutionary Biology (Evogene), Department of Biosciences, University of Oslo, 0316 Oslo, Norway
*
Author to whom correspondence should be addressed.
Academic Editor: Przemysław M. Płonka
Appl. Sci. 2021, 11(3), 1198; https://doi.org/10.3390/app11031198
Received: 22 December 2020 / Revised: 22 January 2021 / Accepted: 25 January 2021 / Published: 28 January 2021
Analytical pyrolysis is a tool widely used for investigating the nature of macromolecules. This technique has been applied with success for solving the chemical structure of geo- and biopolymers. However, with complex macromolecules, such as fungal melanins, analytical pyrolysis presents some drawbacks due to the fact that they rarely can be obtained in a pure grade and contains a mixture of polysaccharides, chitin, proteins, nucleic acids, and lipids, among other materials. Analytical pyrolysis permits the characterization of the compounds co-extracted or linked to the melanin. Nevertheless, our data revealed that under the conditions usually employed in the pyrolysis of melanins, the technique presented serious limitations due to the complex structure and diversity of components that suffer extensive thermal degradation through secondary reactions with the production of considerable number of artifacts. To the light of pyrolysis data, the melanin of Ochroconis spp. is not based on 3,4-dihydroxyphenylalanine (DOPA) or 1,8-dihydroxynaphthalene (DHN), and it is suggested that it could be a type of pyomelanin, based on the polymerization of homogentisic acid and other phenolic compounds. View Full-Text
Keywords: analytical pyrolysis; fungal melanins; Ochroconis lascauxensis; Lascaux Cave; Eurotium echinulatum; Cryptococcus neoformans; DOPA melanin; DHN melanin; pyomelanin analytical pyrolysis; fungal melanins; Ochroconis lascauxensis; Lascaux Cave; Eurotium echinulatum; Cryptococcus neoformans; DOPA melanin; DHN melanin; pyomelanin
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MDPI and ACS Style

Saiz-Jimenez, C.; Martin-Sanchez, P.M.; Gonzalez-Perez, J.A.; Hermosin, B. Analytical Pyrolysis of the Fungal Melanins from Ochroconis spp. Isolated from Lascaux Cave, France. Appl. Sci. 2021, 11, 1198. https://doi.org/10.3390/app11031198

AMA Style

Saiz-Jimenez C, Martin-Sanchez PM, Gonzalez-Perez JA, Hermosin B. Analytical Pyrolysis of the Fungal Melanins from Ochroconis spp. Isolated from Lascaux Cave, France. Applied Sciences. 2021; 11(3):1198. https://doi.org/10.3390/app11031198

Chicago/Turabian Style

Saiz-Jimenez, Cesareo, Pedro M. Martin-Sanchez, Jose A. Gonzalez-Perez, and Bernardo Hermosin. 2021. "Analytical Pyrolysis of the Fungal Melanins from Ochroconis spp. Isolated from Lascaux Cave, France" Applied Sciences 11, no. 3: 1198. https://doi.org/10.3390/app11031198

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