Inhibitory Effect of Cinnamaldehyde on Main Destructive Microorganisms of Nanhai No. 1 Shipwreck
Abstract
:1. Introduction
2. Materials and Methods
2.1. Main Destructive Microorganisms of Nanhai No. 1 Shipwreck
2.2. Revitalizing the Microorganisms
2.3. Determination of Cellulase Activity in Destructive Microorganisms
2.4. Preparation of Bacterial Culture Suspension and Spore Suspension of Fungi
2.5. Disk Diffusion Experiment
2.6. Antimicrobial Experiment of Cinnamaldehyde Volatile Gas
2.7. Determination of Minimum Inhibitory Concentration of Cinnamaldehyde
2.8. Laboratory Simulation Experiments
2.9. Determination of Colony Forming Units(CFU)
2.10. Quantitative Real-Time PCR
3. Results
3.1. Cellulolytic Enzyme Activities of Hull Main Microorganisms
3.2. Determination of Antimicrobial Activity of Cinnamaldehyde by Disc Diffusion Method
3.3. Determination of Antimicrobial Activity of Cinnamaldehyde Volatile Gas in Air
3.4. MIC Determination of Cinnamaldehyde
3.5. Cinnamaldehyde Inhibitory of Major Destructive Microbes of Hull during Laboratory Simulation Experiment
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Strains | Cinnamaldehyde | Euxyl® K100 |
---|---|---|
Bacillus sp. (NK-NH15) | 1 | 0.07813 |
Bacillus sp. (NK-NH16) | 2 | 0.03906 |
Bacillus sp. (NK-NH17) | 1 | 0.03906 |
B. megaterium (NK-NH10) | 0.125 | 0.07813 |
B. velezensis (NK-NH11) | 0.5 | 0.03906 |
B. tequilensis (NK-NH5) | 1 | 0.03906 |
F.solani (NK-NH1) | 0.25 | 0.1563 |
S. apiospermum (NK.W1-3) | 0.0625 | 0.07813 |
N | C1 | C2 | C3 | C4 | |
---|---|---|---|---|---|
CFU/mL | 4.27 × 105 ± 2.04 × 104 | 1.67 × 105 ± 9.02 × 103 | 1.2 × 104 ± 5.89 × 102 | 5.07 × 102 ± 3.86 | 1.2 × 101 ± 5.10 |
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Huang, X.; Han, Y.; Du, J.; Guo, P.; Wang, Y.; Ma, K.; Li, N.; Zhang, Z.; Li, Y.; Pan, J. Inhibitory Effect of Cinnamaldehyde on Main Destructive Microorganisms of Nanhai No. 1 Shipwreck. Appl. Sci. 2021, 11, 5262. https://doi.org/10.3390/app11115262
Huang X, Han Y, Du J, Guo P, Wang Y, Ma K, Li N, Zhang Z, Li Y, Pan J. Inhibitory Effect of Cinnamaldehyde on Main Destructive Microorganisms of Nanhai No. 1 Shipwreck. Applied Sciences. 2021; 11(11):5262. https://doi.org/10.3390/app11115262
Chicago/Turabian StyleHuang, Xinduo, Yeqing Han, Jing Du, Peifeng Guo, Yu Wang, Kaixuan Ma, Naisheng Li, Zhiguo Zhang, Yue Li, and Jiao Pan. 2021. "Inhibitory Effect of Cinnamaldehyde on Main Destructive Microorganisms of Nanhai No. 1 Shipwreck" Applied Sciences 11, no. 11: 5262. https://doi.org/10.3390/app11115262