Next Article in Journal
Flight Strategy Optimization for High-Altitude Solar-Powered Aircraft Based on Gravity Energy Reserving and Mission Altitude
Previous Article in Journal
TLS and GB-RAR Measurements of Vibration Frequencies and Oscillation Amplitudes of Tall Structures: An Application to Wind Towers
Previous Article in Special Issue
Characterization of Biofilm Extracts from Two Marine Bacteria

This is an early access version, the complete PDF, HTML, and XML versions will be available soon.

Open AccessArticle

Marine Microalgae Biomolecules and Their Adhesion Capacity to Salmonella enterica sv. Typhimurium

1
Postgraduate Program in Sciences (Biochemistry), Federal University of Paraná (UFPR), 81531-980 Curitiba, Paraná, Brazil
2
Biochemistry and Molecular Biology Dept., UFPR, Av. Cel. Francisco H. dos Santos, 100, P.O. BOX 19046, 81531-980 Curitiba, Paraná, Brazil
3
Postgraduate Program in Bioprocesses and Biotechnology Engineering, UFPR, Postal code 81531-990, Curitiba, Paraná, Brazil
*
Authors to whom correspondence should be addressed.
These authors equally contributed to this work.
Appl. Sci. 2020, 10(7), 2239; https://doi.org/10.3390/app10072239
Received: 5 March 2020 / Revised: 14 March 2020 / Accepted: 16 March 2020 / Published: 25 March 2020
(This article belongs to the Special Issue Polysaccharides from Marine Environments)
Different molecules have been tested as analog receptors due to their capacity to bind bacteria and prevent cell adhesion. By using in vitro assays, the present study characterized the aqueous and alkaline extracts from microalgae Pavlova lutheri and Pavlova gyrans and evaluated the capacity of these extracts to adhere to enterobacteria (Salmonella Typhimurium). The aqueous and alkaline extracts of both species were fractionated via freeze-thawing, giving rise to soluble and insoluble (precipitate) fractions in cold water. The obtained fractions were studied using thermogravimetric, methylation analyses, and using 1D and 2D NMR techniques. The cold-water-soluble fractions obtained from the aqueous extracts were mainly composed of highly branched (1→3),(1→6)-β-glucans, whereas the cold-water-precipitate fractions were constituted by (1→3)-β-glucans. The alkaline extract fractions showed similar compositions with a high protein content, and the presence of glycosides (sulfoquinovosylglycerol (SQG), digalactosylglycerol (DGG)), and free fatty acids. The linear (1→3)-β-glucans and the alkaline extract fractions showed an adhesion capacity toward Salmonella. The chemical composition of the active fractions suggested that the presence of three-linked β-glucose units, as well as microalgal proteins and glycosides, could be important in the adhesion process. Therefore, these microalgal species possess a high potential to serve as a source of anti-adhesive compounds.
Keywords: microalgae; Pavlova; β-glucans; glycosides; adhesion capacity; enterobacteria microalgae; Pavlova; β-glucans; glycosides; adhesion capacity; enterobacteria
MDPI and ACS Style

Machado, T.W.M.; Rodrigues, J.M.; Moro, T.R.; Duarte, M.E.R.; Noseda, M.D. Marine Microalgae Biomolecules and Their Adhesion Capacity to Salmonella enterica sv. Typhimurium. Appl. Sci. 2020, 10, 2239.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop