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Open AccessArticle

Marine Microalgae Biomolecules and Their Adhesion Capacity to Salmonella enterica sv. Typhimurium

Postgraduate Program in Sciences (Biochemistry), Federal University of Paraná (UFPR), 81531-980 Curitiba, Paraná, Brazil
Biochemistry and Molecular Biology Dept., UFPR, Av. Cel. Francisco H. dos Santos, 100, P.O. BOX 19046, 81531-980 Curitiba, Paraná, Brazil
Postgraduate Program in Bioprocesses and Biotechnology Engineering, UFPR, Postal code 81531-990, Curitiba, Paraná, Brazil
Authors to whom correspondence should be addressed.
These authors equally contributed to this work.
Appl. Sci. 2020, 10(7), 2239;
Received: 5 March 2020 / Revised: 14 March 2020 / Accepted: 16 March 2020 / Published: 25 March 2020
(This article belongs to the Special Issue Polysaccharides from Marine Environments)
Different molecules have been tested as analog receptors due to their capacity to bind bacteria and prevent cell adhesion. By using in vitro assays, the present study characterized the aqueous and alkaline extracts from microalgae Pavlova lutheri and Pavlova gyrans and evaluated the capacity of these extracts to adhere to enterobacteria (Salmonella Typhimurium). The aqueous and alkaline extracts of both species were fractionated via freeze-thawing, giving rise to soluble and insoluble (precipitate) fractions in cold water. The obtained fractions were studied using thermogravimetric, methylation analyses, and using 1D and 2D NMR techniques. The cold-water-soluble fractions obtained from the aqueous extracts were mainly composed of highly branched (1→3),(1→6)-β-glucans, whereas the cold-water-precipitate fractions were constituted by (1→3)-β-glucans. The alkaline extract fractions showed similar compositions with a high protein content, and the presence of glycosides (sulfoquinovosylglycerol (SQG), digalactosylglycerol (DGG)), and free fatty acids. The linear (1→3)-β-glucans and the alkaline extract fractions showed an adhesion capacity toward Salmonella. The chemical composition of the active fractions suggested that the presence of three-linked β-glucose units, as well as microalgal proteins and glycosides, could be important in the adhesion process. Therefore, these microalgal species possess a high potential to serve as a source of anti-adhesive compounds.
Keywords: microalgae; Pavlova; β-glucans; glycosides; adhesion capacity; enterobacteria microalgae; Pavlova; β-glucans; glycosides; adhesion capacity; enterobacteria
MDPI and ACS Style

Machado, T.W.M.; Rodrigues, J.M.; Moro, T.R.; Duarte, M.E.R.; Noseda, M.D. Marine Microalgae Biomolecules and Their Adhesion Capacity to Salmonella enterica sv. Typhimurium. Appl. Sci. 2020, 10, 2239.

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