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Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

1
Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, 205 06 Malmö, Sweden
2
Biofilms-Research Center for Biointerfaces, Malmö University, 205 06 Malmö, Sweden
3
Phase Holographic Imaging AB, 223 63 Lund, Sweden
4
Department of Translational Medicine, Lund University, 205 06 Malmö, Sweden
5
Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden
*
Author to whom correspondence should be addressed.
Appl. Sci. 2020, 10(14), 4854; https://doi.org/10.3390/app10144854
Received: 29 May 2020 / Revised: 13 July 2020 / Accepted: 13 July 2020 / Published: 15 July 2020
(This article belongs to the Special Issue Applications of Digital Holography in Biomedical Engineering)
Breast cancer is the second most common cancer worldwide. Metastasis is the main reason for death in breast cancer, and today, there is a lack of methods to detect and isolate circulating tumor cells (CTCs), mainly due to their heterogeneity and rarity. There are some systems that are designed to detect rare epithelial cancer cells in whole blood based on the most common marker used today, the epithelial cell adhesion molecule (EpCAM). It has been shown that aggressive breast cancer metastases are of non-epithelial origin and are therefore not always detected using EpCAM as a marker. In the present study, we used an in vitro-based circulating tumor cell model comprising a collection of six breast cancer cell lines and white blood cell lines. We used digital holographic cytometry (DHC) to characterize and distinguish between the different cell types by area, volume and thickness. Here, we present significant differences in cell size-related parameters observed when comparing white blood cells and breast cancer cells by using DHC. In conclusion, DHC can be a powerful diagnostic tool for the characterization of CTCs in the blood. View Full-Text
Keywords: breast cancer; cell area; cell thickness; cell volume; circulating tumor cell; CD45; digital holographic cytometry; EpCAM breast cancer; cell area; cell thickness; cell volume; circulating tumor cell; CD45; digital holographic cytometry; EpCAM
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MDPI and ACS Style

El-Schich, Z.; Janicke, B.; Alm, K.; Dizeyi, N.; L. Persson, J.; Gjörloff Wingren, A. Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry. Appl. Sci. 2020, 10, 4854. https://doi.org/10.3390/app10144854

AMA Style

El-Schich Z, Janicke B, Alm K, Dizeyi N, L. Persson J, Gjörloff Wingren A. Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry. Applied Sciences. 2020; 10(14):4854. https://doi.org/10.3390/app10144854

Chicago/Turabian Style

El-Schich, Zahra; Janicke, Birgit; Alm, Kersti; Dizeyi, Nishtman; L. Persson, Jenny; Gjörloff Wingren, Anette. 2020. "Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry" Appl. Sci. 10, no. 14: 4854. https://doi.org/10.3390/app10144854

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