Evaluation of the Effectiveness of N-Acetylcysteine (NAC) and N-acetylcysteine-cyclodextrins Multi-Composite in Pseudomonas aeruginosa Biofilm Formation
Abstract
:1. Introduction
2. Materials
3. Methods
3.1. Analytical Quantification of N-Acetylcysteine within NACESOLTM
3.2. Chemico-Physical Characterization
3.2.1. Thermal Analysis: Differential Scanning Calorimetry (DSC)
3.2.2. X-ray Powder Diffraction (XRPD) Measurements
3.2.3. Fourier-Transform Infrared (FTIR) Spectroscopy Measurements
3.3. Biofilm Assay
3.3.1. Microbial Strains and Culture Conditions
3.3.2. Biofilm Growth Protocol
3.3.3. Prevention of Biofilm Formation Assay
3.4. Statistical Analysis
3.5. Scanning Electron Microscopy (SEM)
4. Results
4.1. Analytical Quantification of N-Acetylcysteine within NACESOLTM
4.2. Chemico-Physical Characterization
4.2.1. DSC Measurements
4.2.2. XRPD Measurements
4.2.3. FT-IR Measurements
4.3. Biofilm Assay
4.3.1. Biofilm Growth Protocol
4.3.2. Biofilm Formation Assay
4.4. Scanning Electron Microscope and Elementary Analysis
5. Discussion
- Stabilization to oxidation;
- Protection against degradation of substances or by microorganisms;
- Masking of the smell;
- Catalytic activity of CDs with guest molecule.
6. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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S Content (%) | S.D. | |
---|---|---|
CTR | 0.43 | 0.018 |
ACTIVE | 0.24 | 0.095 |
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Guerini, M.; Perugini, P.; Grisoli, P. Evaluation of the Effectiveness of N-Acetylcysteine (NAC) and N-acetylcysteine-cyclodextrins Multi-Composite in Pseudomonas aeruginosa Biofilm Formation. Appl. Sci. 2020, 10, 3466. https://doi.org/10.3390/app10103466
Guerini M, Perugini P, Grisoli P. Evaluation of the Effectiveness of N-Acetylcysteine (NAC) and N-acetylcysteine-cyclodextrins Multi-Composite in Pseudomonas aeruginosa Biofilm Formation. Applied Sciences. 2020; 10(10):3466. https://doi.org/10.3390/app10103466
Chicago/Turabian StyleGuerini, Marta, Paola Perugini, and Pietro Grisoli. 2020. "Evaluation of the Effectiveness of N-Acetylcysteine (NAC) and N-acetylcysteine-cyclodextrins Multi-Composite in Pseudomonas aeruginosa Biofilm Formation" Applied Sciences 10, no. 10: 3466. https://doi.org/10.3390/app10103466