Next Article in Journal
Report from the Scientific Poster Session at the 12th Annual Cardiometabolic Health Congress in Boston, USA, 4–7 October 2017
Previous Article in Journal
Regulation of Polyamine Metabolism by Curcumin for Cancer Prevention and Therapy
Article Menu

Export Article

Open AccessFeature PaperArticle
Med. Sci. 2017, 5(4), 35; https://doi.org/10.3390/medsci5040035

cGAMP Promotes Germinal Center Formation and Production of IgA in Nasal-Associated Lymphoid Tissue

1
Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido University, Kita 15, Nishi 7, Kita-ku, Sapporo 060-8638, Japan
2
Department of Human Immunology, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, South 1, West 17, Chuo-ku, Sapporo 060-8556, Japan
3
Department of Immunology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1, Honjyo, Chuou-ku, Kumamoto 860-0811, Japan
4
Department of Pathology, National Institute of Infectious Diseases, 1-23-1, Toyama, Shinjuku, Tokyo 162-8640, Japan
*
Authors to whom correspondence should be addressed.
Received: 13 November 2017 / Revised: 11 December 2017 / Accepted: 11 December 2017 / Published: 18 December 2017
(This article belongs to the Section Immunology and Infectious Diseases)
Full-Text   |   PDF [1557 KB, uploaded 18 December 2017]   |  
  |   Review Reports

Abstract

Induction of immunoglobulin (Ig) A in the mucosa of the upper respiratory tract and the nasal cavity protects against influenza virus infection. Cyclic dinucleotides (CDNs) are used as mucosal adjuvants to enhance the immunogenicity of intranasal influenza hemagglutinin (HA) vaccines. The adjuvant activity of 2′3′ cyclic guanosine monophosphate–adenosine monophosphate (cGAMP) on Ig production was investigated in nasal-associated lymphoid tissue (NALT), serum of wild-type C57BL/6J, and stimulator of interferon genes (STING)-deficient mice, which do not recognize cGAMP. Mice were vaccinated intranasally with a HA vaccine with or without the cGAMP adjuvant. IgA and IgG production, T-cell responses, germinal center formation, and cytokine expression in NALT were assayed. cGAMP enhanced IgA and IgG production, and promoted T-cell responses. Intranasal administration of cGAMP activated both NALT and systemic immune cells, induced a favorable cytokine environment for IgA induction, and promoted germinal center formation. The cGAMP effect was STING-dependent. Taken together, cGAMP as an HA vaccine adjuvant promoted a STING-dependent NALT environment suitable for the enhancement of IgA production. View Full-Text
Keywords: IgA; NALT; cGAMP; STING IgA; NALT; cGAMP; STING
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Takaki, H.; Takashima, K.; Oshiumi, H.; Ainai, A.; Suzuki, T.; Hasegawa, H.; Matsumoto, M.; Seya, T. cGAMP Promotes Germinal Center Formation and Production of IgA in Nasal-Associated Lymphoid Tissue. Med. Sci. 2017, 5, 35.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Med. Sci. EISSN 2076-3271 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top